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EC number: 269-924-1 | CAS number: 68391-05-9 This substance is identified by SDA Substance Name: C12-C18 dialkyl dimethyl ammonium chloride and SDA Reporting Number: 16-047-00.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From February 22, 2010 to February 24, 2010
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- No chemical analyses of the test concentrations were performed during the test and all concentrations mentioned are nominal concentrations of the test substance.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- not required
- Details on sampling:
- No data
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The stock solution was prepared by stirring 0.46 g quaternary ammonium compounds, di-C12-18-alkyldimethyl, chlorides in 460 mL of deionized water. The stock solution was a stable homogenous suspension (very light turbidity).
- pH stock solution = 5.0 - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture: Unadapted secondary activated sludge was obtained (22-02-2010) from the WWTP Nieuwgraaf in Duiven. The WWTP Duiven is an activated sludge plant treating predominantly domestic wastewater.
- Method of cultivation: Prior to use the activated sludge was homogenized with a syringe. The dry weight of the
homogenized activated sludge was determined and subsequently concentrated by settlement to the required dry weight concentration.
- Preparation of inoculum for exposure: During the test period the activated sludge was kept in a thermostated 2 L cylindrical glass vessel to maintain the temperature at 20 ± 2 °C. A magnetic stirrer bar at the bottom and a glass frit were used to agitate and aerate the activated sludge. The dry weight of the activated sludge in the incubation vessels was 1.7 g/L.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- No data
- Hardness:
- No data
- Test temperature:
- 20.2- 21.0 °C
- pH:
- 6.9- 7.2
- Dissolved oxygen:
- No data
- Salinity:
- No data
- Nominal and measured concentrations:
- Nominal: 0, 10, 20, 40, 80, 160 and 320 mg/L
- Details on test conditions:
- Test conditions
The test was performed in 0.25 to 0.30 liter Erlenmeyer flasks with a total working volume of 50 mL. The homogenized activated sludge was incubated in a shaking water bath (100 rpm, 20°C) for 3 hours with various concentrations of the test compound and synthetic sewage. Aeration of the activated sludge during this period was achieved by agitation.
Synthetic sewage feed and stocks
The synthetic sewage feed was made by dissolving the following amounts of substances in 1 liter of deionized water: 16 g peptone, 11 g meat extract, 3 g urea, 0.7 g NaCl, 0.3 g CaCl2, 0.2 g MgSO4.7H2O, 2.8 g K2HPO4. In the incubation vessels 1.6 mL of the synthetic sewage feed is added in a total volume of 50 mL. The test substance and 3,5-dichlorophenol were added to the incubation vessels using stock solutions of approximately 1.0 and 0.5 g/L respectively. The stock solution of 3,5- dichlorophenol was prepared by dissolving 0.0508 g 3,5-dichlorophenol in 1 mL of 1 M NaOH. Then diluting this to approximately 30 mL with deionized water, adding under stirring 0.5 M H2SO4 to the point of incipient precipitation and finally diluting the mixture to 100 milliliter with deionized water. The test substance stock solution was prepared by stirring 0.46 g test substance in 460 mL of deionized water. The test substance stock solution was a stable homogenous suspension (very light turbidity). - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 68 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: with 95% confidence limits of 15 and 858 mg/L.
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 12 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- The inhibitory effect of test substance at a particular concentration is expressed as a percentage of the two controls. From the results EC values were calculated. The EC50 of test substance for activated sludge after 3 hours contact time was 68 mg/L with 95% confidence limits of 15 and 858 mg/L. The EC10, EC20 and EC80 were 12, 22 and 215 mg/L, respectively. The test substance is therefore considered harmful to activated sludge. The validity of the test is demonstrated by two criteria. First, the control respiration rates were within 15% of each other and secondly, the EC50 of the reference compound was 8 mg/L which is within the prescribed range of 5 to 30 mg/L.
- Results with reference substance (positive control):
- The EC50 of the reference compound was 8 mg/L which is within the prescribed range of 5 to 30 mg/L
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the study conditions, the 3 h EC50 and EC10 of the test substance for activated sludge was determined to be 68 and 12 mg/L respectively.
- Executive summary:
A study was conducted to determine an activated sludge respiration inhibition potential of the test substance, C12-18 DAQ (99.6% active), according to OECD Guideline 209, in compliance with GLP. The toxicity of the test substance to activated sludge was determined at a contact time of 3 h, using the test substance at concentrations of 10, 20, 40, 80 and 160 mg/L. The inhibitory effect of the test substance at a particular concentration is expressed as a percentage of the two controls.No chemical analyses of the test concentrations were performed during the test and all concentrations mentioned are nominal concentrations of the test substance.In the study, the EC50, EC10, EC20 and EC80 of the test substance for activated sludge after 3 h contact time were determined to be 68 mg/L (95% CL: 15 and 858 mg/L), 12, 22 and 215 mg/L, respectively. The control respiration rates were within 15% of each other and the EC50 of the reference compound was 8 mg/L which is within the prescribed range of 5 to 30 mg/L. Therefore, the validity of the study was demonstrated. Under the study conditions, the 3 h EC50 and EC10 of the test substance for activated sludge were determined to be 68 and 12 mg/L (nominal) respectively (Geerts, 2010).
Reference
Results
Table I Respiration rates of the activated sludge, inhibition percentages, and pH at various concentrations of 3,5-dichlorophenol after 3 hours contact time
Concentration mg/L |
Activity mg O2/g.min |
Inhibition% |
pH start test |
pH end test |
Temperature end test (°C) |
Control |
0.30 |
- |
6.8 |
7.0 |
20.4 |
Control |
0.30 |
- |
6.8 |
7.0 |
20.6 |
Control* |
0.28 |
- |
6.7 |
6.9 |
20.8 |
Control* |
0.26 |
- |
6.7 |
6.9 |
20.8 |
1.3* |
0.24 |
10 |
6.7 |
7.0 |
20.9 |
2.5 |
0.23 |
24 |
6.8 |
7.2 |
20.2 |
5.1 |
0.20 |
34 |
6.8 |
7.1 |
20.7 |
10.2 |
0.13 |
55 |
6.8 |
7.1 |
20.3 |
20.3 |
0.06 |
79 |
6.8 |
7.1 |
20.4 |
40.6 |
0.03 |
88 |
6.8 |
7.1 |
20.3 |
Concentration stock solution 3,5-dichlorophenol = 508 mg/L pH stock solution 3,5-dichlorophenol = 7.0
* = corresponding control and reference concentration
Table II Respiration rates of the activated sludge, inhibition percentages and pH at various concentrations of test substance after 3 hours contact time
Concentration mg/L |
Activity mg O2/g.min |
Inhibition% |
pH start test |
pH end test |
Temperature end test (°C) |
Control |
0.45 |
- |
6.9 |
7.1 |
20.5 |
Control |
0.45 |
- |
6.9 |
7.1 |
20.7 |
Control* |
0.42 |
- |
6.8 |
7.1 |
21.0 |
Control* |
0.42 |
- |
6.8 |
7.0 |
20.5 |
10 |
0.41 |
10 |
6.9 |
7.1 |
20.5 |
20 |
0.37 |
17 |
6.9 |
7.1 |
20.5 |
40 |
0.30 |
33 |
6.9 |
7.1 |
20.5 |
80 |
0.23 |
50 |
6.9 |
7.1 |
20.6 |
160 |
0.12 |
74 |
6.9 |
7.1 |
20.5 |
320* |
0.05 |
89 |
6.8 |
7.0 |
20.7 |
Concentration stock solutiontest substance= 1 g/L
pH stock solution test substance = 5.0
* = corresponding control and reference concentration
Description of key information
Based on the study results, the 3 h EC50 and EC10 of the test substance for activated sludge was determined to be 68 and 12 mg/L (nominal) respectively.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 68 mg/L
- EC10 or NOEC for microorganisms:
- 12 mg/L
Additional information
A study was conducted to determine an activated sludge respiration inhibition potential of the test substance, C12-18 DAQ (99.6% active), according to OECD Guideline 209, in compliance with GLP. The toxicity of the test substance to activated sludge was determined at a contact time of 3 h, using the test substance at concentrations of 10, 20, 40, 80 and 160 mg/L. The inhibitory effect of the test substance at a particular concentration is expressed as a percentage of the two controls. No chemical analyses of the test concentrations were performed during the test and all concentrations mentioned are nominal concentrations of the test substance. In the study, the EC50, EC10, EC20 and EC80 of the test substance for activated sludge after 3 h contact time were determined to be 68 mg/L (95% CL: 15 and 858 mg/L), 12, 22 and 215 mg/L, respectively. The control respiration rates were within 15% of each other and the EC50 of the reference compound was 8 mg/L which is within the prescribed range of 5 to 30 mg/L. Therefore, the validity of the study was demonstrated. Under the study conditions, the 3 h EC50 and EC10 of the test substance for activated sludge were determined to be 68 and 12 mg/L (nominal) respectively (Geerts, 2010).
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