Registration Dossier
Registration Dossier
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EC number: 221-264-5 | CAS number: 3049-71-6
Endpoint summary
Administrative data
Description of key information
The test substance is with high probability acutely not harmful to aquatic organisms.
Additional information
The test substance is with high probability acutely not harmful to aquatic organisms.
The inhibition of the degradation activity of activated sludge is not anticipated when introduced in appropriate low concentrations.
The test substance belongs to the category of perylene as indicated by its chemical core structure. Based on this structure and the chemical and biological stability of perylenes under environmental conditions, data from structural analogue perylenes can be used to cover this endpoint required under REACh if no data for the original test substance is available.
In a GLP study following OECD guideline 203 the acute toxicity of the analogue perylene CAS 4948-15-6 to fish was determined. Test fish of the species Brachydanio rerio were exposed to the substance for 96 hours in a static test system. At test termination a LC50 > 1350 mg/L based on measured test substance concentrations was determined [BASF AG, 1999].
A study on the toxicity of the analogue substance CAS 81-33-4 to Daphnia magna has been conducted according to OECD guideline 202 following a static test system setup [BASF SE, 2012]. At test termination after 48 h no toxicity was observed up to the limit of solubility of the test substance (EC50 > 0.0065 mg/L; measured).
The acute toxicity of the test substance to aquatic invertebrates has been examined in a GLP guideline study according to EEC Directive 92/32/EEC. The test animals of the species Daphnia magna were exposed to the test substance for 48 h in a static test system set up. At test termination an EC50 > 100 mg/l was determined [BASF AG, 1998]. Similar results were obtained for Perylen Schwarz/Black I (EC 479-300-2) and Perylen Schwarz/Black II (EC 475-310-6) were toxicity to Daphnia magna was examined in 48 hour static limit tests according to OECD guideline 202, respectively. At test termination an EC50 of > 100 mg/l based on nominal test substance concentrations was determined, respectively (BASF AG 2005, 2006).
The toxicity of the analogue substance CAS 4948-15-6 on aquatic algae was investigated in a GLP guideline study according to OECD 201 with Pseudokirchneriella subcapitata [BASF SE, 2012]. The substance had no observable inhibitory effect on algae growth up to its saturation limit in the test media and under test conditions. The ErC50 value after 72 h of exposure was determined to be > 100 mg/L (nominal). The inhibiting abilities of Perylen Schwarz/Black I (EC 479-300-2) and Perylen Schwarz/Black II (EC 475-310-6) to Pseudokirchnerella subcapitata were examined in GLP studies according to OECD guideline 201, respectively. After 72 h the EC50 value for Perylen Schwarz/Black I and Perylen Schwarz/Black II was determined to be > 100 mg/L based on the nominal concentration of the test substances (BASF AG 2006). No effect was observed up to the solubility limit of the test substance in water.
The acute toxicity of the analogue substance CAS 81-33-4 to aquatic plants has been examined in a GLP guideline study according to OECD 221 with the test species Lemna gibba [BASF SE, 2012]. At test termination after 7 d, an ECr50 > 0.007 mg/L (measured) relating to growth rate (frond rate and dry weight, respectively) was determined.
Oxygen consumption test which were conducted according to ROBRA (DIN 38412, part 27) within bacteria Pseudomonas putida was exposed to different test concentrations of the test substance [BASF AG 1990]. After an exposure period of 30 minutes the lowest determined effect concentration was > 10000 mg/L (nominal).
All tests were run with concentrations above the water solubility of the test substance and its analogues, respectively. Thus, no effects in the range of the water solubility of the substance were determined.
The hazard assessment of the substance reveals neither a need to classify the substance as dangerous to the environment, nor is it a PBT or vPvB substance, nor are there any further indications that the substance may be hazardous to the environment. Therefore, and for reasons of animal welfare long-term toxicity testing in fish and aquatic invertebrates is not provided.
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