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EC number: 208-796-3 | CAS number: 542-02-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- July - October 1972
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: No guideline for the testing methode is mentioned in the report. Nevertheless the test result seems to be reliable as the observation period is also stated to be 14 days and the testing procedure as well as the choice of doses seems to be reasonable.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 972
- Report date:
- 1972
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- GLP compliance:
- not specified
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 2,4-Diamino-6-methyl-1,3,5-triazine
- IUPAC Name:
- 2,4-Diamino-6-methyl-1,3,5-triazine
- Reference substance name:
- 6-methyl-1,3,5-triazine-2,4-diyldiamine
- EC Number:
- 208-796-3
- EC Name:
- 6-methyl-1,3,5-triazine-2,4-diyldiamine
- Cas Number:
- 542-02-9
- Molecular formula:
- C4H7N5
- IUPAC Name:
- 6-methyl-1,3,5-triazine-2,4-diamine
- Details on test material:
- - Name of test material (as cited in study report): Acetoguanamine
- Appearance/physical state/colour: white powder
Constituent 1
Constituent 2
Test animals
- Species:
- rat
- Strain:
- other: Wistar derived
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: colony of TNO institute
- Weight at study initiation: 150 to 260 g (males) and 108 to 208 g (females)
- Fasting period before study: 16 hours
- Stock diet and tap water: ad libitum
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- CMC (carboxymethyl cellulose)
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: a 20 % (w/v) suspension of Acetoguanamine in a 5 % aqueous solution of carboxy methyl cellulose
MAXIMUM DOSE VOLUME APPLIED:
15 ml of the 20 % suspension of Acetoguanamine in a 5 % aqueous solution of carboxy methyl cellulose for each kg of body weight. - Doses:
- 10, 11, 12, 13, 14 and 15 ml of the 20 % suspension of Acetoguanamine in a 5 % aqueous solution of carboxy methyl cellulose for each kg of body weight.
- No. of animals per sex per dose:
- five
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes - Statistics:
- no statistics reported
Results and discussion
Effect levels
- Sex:
- not specified
- Dose descriptor:
- LD50
- Effect level:
- 2.74 other: g/kg
- 95% CL:
- 2.57 - 2.92
- Mortality:
- Some of the animals died within three hours after the treatment with Acetoguanamine.
Deaths occurred up to two days after dosing. - Clinical signs:
- other: One hour after the treatment all animals became sluggish and showed severe diarrhoea. But the survivors recovered rapidly and looked quite healthy again at the end of the first week.
- Gross pathology:
- No abnormalities were seen in these animals at autopsy.
- Other findings:
- no data
Any other information on results incl. tables
TABEL 1: The mortality in the test animals of the various dose groups after the treatment with Acetoguanamine.
dose |
mortality |
|||
ml 20 % solution/kg |
g test substance/kg |
number |
% |
|
males |
females |
|||
10 |
2.0 |
0/5 |
0/5 |
0 |
11 |
2.2 |
0/5 |
2/5 |
20 |
12 |
2.4 |
1/5 |
0/5 |
10 |
13 |
2.6 |
0/5 |
2/5 |
20 |
14 |
2.8 |
2/5 |
4/5 |
60 |
15 |
3.0 |
3/5 |
5/5 |
80 |
Applicant's summary and conclusion
- Interpretation of results:
- not classified
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The LD50 value of Acetoguanamine was calculated to be 2.74 g/kg body weight with 2.92 and 2.57 as 95 % confidence limits. Because of this reason Acetoguanamine is not classified as acute oral toxic.
- Executive summary:
An acute oral toxicity test with Acetoguanamine was performed. No guideline for the testing methode is mentioned in the report. Nevertheless the test result seems to be reliable as the observation period is also stated to be 14 days and the testing procedure as well as the choice of doses seems to be reasonable.
On the basis of some preliminary observations the following doses were given as as one single dose to groups of five male and five female rats: 2.0, 2.2, 2.4, 2.6, 2.8, 3.0 g test substance / kg bw.
The animals were observed for a 14 -day period. Then the survivors were killed and examined grossly. With the obtained study results the LD50 of Acetoguanamine was calculated to be 2.74 g/kg body weight. Because of this reason Acetoguanamine is not classified as acute oral toxic.
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