trisodium 2-(2-sulfoethoxy)ethane-1-sulfonate ethenesulfonate

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: screening test, other

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2022

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

yes

Remarks:

The study was extended until 60% of degradation was obtained

GLP compliance:

yes

Specific details on test material used for the study:

Experiment 1.

Reaction mass of disodium 2,2 oxydiethanesulfonate and sodium ethenesulfonate
Batch lot number:20210830033 used for experimant 1
Dry weight content : 33.65 %, correction factor is 2.971 The test item is a multi-constituent Composition.
Constituent1:Sodium ethylene sulphonate cas 3039-83-6: 75.01 %
Constituent 2 : Isethionate bisether, disodium salt cas 63440-92-6: 16.52%
Impurity 1:Isethionate sodium cas 1562-00-1: 4.12%
Impurity 2:Sodium ethionate, disodium salt cas1562-03-4: 0.62 %
Impurity 3:Sodium sulfate cas 7757-82-6 :2.76%
Impurity 4:Sodium ethandisulfonate disodium salt cas 5325-43-9:0.64 %

Experiment 2.
Reaction mass of disodium 2,2 oxydiethanesulfonate and sodium ethenesulfonate
Batch lot number:202206140027 used for experimant 2
Dry weight content : 33.94 %, correction factor is 2.94 The test item is a multi-constituent
Composition.
Constituent1:Sodium ethylene sulphonate cas 3039-83-6: 74.84 %
Constituent 2 : Isethionate bisether, disodium salt cas 63440-92-6: 15.18%
Impurity 1:Isethionate sodium cas 1562-00-1: 5.15%
Impurity 2:Sodium ethionate, disodium salt cas1562-03-4: 0.48 %
Impurity 3:Sodium sulfate cas 7757-82-6 :3.12 %
Impurity 4:Sodium ethandisulfonate disodium salt cas 5325-43-9:0.49 %

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

Source:
The source of test organisms is activated sludge freshly obtained from a municipal sewage
treatment plant, receiving predominantly domestic sewage, located in the vicinity of the testing
facility. Name and exact location will be specified in the report. In general, this will be
'Waterschap Aa en Maas', 's- Hertogenbosch, The Netherlands.

Treatment
The freshly obtained sludge will be coarsely sieved (≥ 1 mm) and kept under continuous aeration
until further treatment. The sludge will be homogenized using a blender on medium speed.
Before use, the concentration of suspended solids (SS) in the activated sludge will be determined
in order to calculate the amount of sludge needed to achieve a final SS concentration of
approximately but not exceeding 30 mg/L. Actual SS concentration will be specified in the
report.

Duration of test (contact time):

ca. 60 d

Initial conc.:

ca. 116 mg/L

Based on:

ThOD

Remarks:

based on a ThOD of 29.2

Initial conc.:

ca. 345 mg/L

Based on:

ThOD

Remarks:

Based on a ThoD of 82 mg O2/L

Parameter followed for biodegradation estimation:

CO2 evolution

Remarks:

A CO2 absorber containing Soda lime pellets (Sigma -Aldrich Steinheim Germany) is used.

Details on study design:

TEST CONDITIONS
- Composition of medium:
1 L mineral medium contains: 10 mL of solution (A),
1 mL of solutions (B) through (D) and Milli-RO water.
Mineral components:

A) 8.50 g KH2PO4
21.75 g K2HPO4
67.20 g Na2HPO4.12H2O
0.50 g NH4Cl
dissolved in Milli-RO water and made up to 1 L, pH 7.4 ± 0.2
B) 22.50 g MgSO4.7H2O dissolved in Milli-RO water and
made up to 1 L.
C) 36.40 g CaCl2.2H2O dissolved in Milli-RO water and
made up to 1 L.
D) 0.25 g FeCl3.6H2O dissolved in Milli-RO water and
made up to 1 L.
- Additional substrate: not used
- Solubilising agent (type and concentration if used):not used
- Test temperature: 22 +/- 1°C monitored
- pH: monitored
- pH adjusted: yes/no
- CEC (meq/100 g):
- Aeration of dilution water: no
- Suspended solids concentration: ss of 30 mg/L

- Continuous darkness: yes
- Other:

TEST SYSTEM
- Culturing apparatus:
- Number of culture flasks/concentration:
- Method used to create aerobic conditions:
- Method used to create anaerobic conditions: not anaerobic
- Measuring equipment:
Oxygen consumption Lovibond BD600-GLP manometric respirometry system, equipped with an inductive stirring system.
pH and temperature measurement
- Test performed in closed vessels due to significant volatility of test substance: yes closed vessels
- Test performed in open system: no
- Details of trap for CO2 and volatile organics if used:
The CO2 produced in each test vessel reacted with the CO2 absorbent in the rubber gaskets. As gaseous O2 was converted into gaseous CO2 that was absorbed, the gas pressure in the test system slowly decreased. This decrease in air pressure was measured by the respirometer heads and automatically converted into oxygen consumption (mg O2/L).(Appendix 3 and 4)
Measurements were recorded automatically for the duration of the study.
- Other:

SAMPLING
- Sampling frequency: continuous monitoring of the gas pressure with a respirometer, and automatically converted into oxygen consumption (mg O2/L).
- Sampling method: no samples are taken
- Sterility check if applicable: not applicable
- Sample storage before analysis: no samples taken
- Other:

CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
- Abiotic sterile control: no
- Toxicity control: yes
- Other: Adsorption control

STATISTICAL METHODS: not used

Reference substance:

benzoic acid, sodium salt

Key result

Parameter:

% degradation (CO2 evolution)

Value:

ca. 87

Sampling time:

28 d

Remarks on result:

other: Experiment 1 conducted at 115 mg/L

Key result

Parameter:

% degradation (CO2 evolution)

Value:

> 60

Sampling time:

35 d

Remarks on result:

other: Experiment 2 conducted at a start concentration of the test material of 345 mg/L

Results with reference substance:

Experiment 1: 92 % degradation after 14 days
Experiment 2: 85 % degradation after 14 days

Validity criteria fulfilled:

yes

Interpretation of results:

inherently biodegradable

Remarks:

Combining the results from both experiments, it can be concluded that Reaction mass of disodium 2,2’-oxydiethanesulfonate and sodium ethenesulfonate degrades rapidly and is clearly inherently biodegradable.Furthermore, then test material may qualify to be labelled as readily biodegradable based on the results of the first experiment.

Conclusions:

First experiment.
The relative biodegradation values calculated from the measurements performed during the test period of the first experiment revealed 88 % and 86 % biodegradation of test material within 28 days, for vessel A and B, respectively (based on ThOD). The criterion for ready biodegradability was met, since biodegradation of at least 60 % was reached within 28 days. Biodegradation further increased to 116 % and 117 % by Day 60, for vessel A and B, respectively.
In the toxicity control, more than 25 % biodegradation occurred within 14 days (71 %, based on ThOD). Therefore, the test material was considered not to inhibit microbial activity.
Functioning of the test system was checked by testing the procedural control material sodium benzoate, which showed a normal biodegradation curve


Second experiment.
The relative biodegradation values calculated from the measurements performed during the test period of the second experiment revealed between 61 % and 77 % biodegradation (72 % on average) within 35 days (based on ThOD). Average biodegradation on Day 28 was 40 %. Since biodegradation of the test material of at least 60 % was not reached within 28 days, the criterion for ready biodegradability was not met.In the toxicity control, more than 25 % biodegradation occurred within 14 days (47 %, based on ThOD). Therefore, the test material was considered not to inhibit microbial activity.
Functioning of the test system was checked by testing the procedural control material sodium benzoate, which showed a normal biodegradation curve

Executive summary:

The results of the first experiment meet the criteria to label the test material as readily biodegradable, although the test material concentration is lower than recommended in the OECD 301F TG. Both vessels exceed the 100 % level, which is partially caused by the relatively low ThOD per vessel, since this leads to an unfavourable signal-to-noise ratio. Results up to 110 % biodegradation are normally considered to be scientifically defensible. Considering the relatively low ThOD in the test vessels of the first experiment, stretching this threshold to 120 % can be justified. This reasoning can be supported by evaluating the difference between the two blanks on Day 60 (actual value: 6 mg O₂/L), which would translate to approximately 20 % biodegradation at a ThOD slightly below 30 mg O₂/L. Subtracting this theoretical value from the biodegradation percentages obtained for the test material vessels would lead to 95 % and 96 % biodegradation in vessel A and B, respectively.

The results of the second experiment show a longer lag phase than the first experiment, which could indicate that the lag phase is concentration-dependent. Biodegradation after 28 days is <60 % (actual average value: 40 %), which does not satisfy the criteria to label the test material as readily biodegradable. However, by Day35  >60 % biodegradation is observed in each replicate. Since these results mean that the pass level criterion was fulfilled but not within 28 days, the results can be used to support inherent biodegradability.

Combining the results from both experiments, it can be concluded that Reaction mass of disodium 2,2’-oxydiethanesulfonate and sodium ethenesulfonate degrades rapidly and is clearly inherently biodegradable.Furthermore, then test material may qualify to be labelled as readily biodegradable based on the results of the first experiment.

 

 

 

Description of key information

The aerobic biodegradation of Reaction mass of disodium 2,2’-oxydiethanesulfonate and sodium ethenesulfonate  was experimental  measured  following  the  OECD TG3.1 E ( Ready . Under  the test conditions  of OECD  301 E Reaction  mass of disodium 2,2 oxydiethanesulfonate and sodium ethenesulfonate  was biodegraded for 56 %  after 28 days. In conclusion, Reaction mass of disodium 2,2’-oxydiethanesulfonate and sodium ethenesulfonate (700-978-5) was not readily biodegradable under the conditions of test.

In oder to evaluate whether the  test substance can meet the  60% of biodegradation  within 60 days (criteria for persistent). Two additional experiments were conducted according to the OECD 301 F. In the first experiment (115 mg/L start  concentration)  87 % degradation was achieved after 28 days. In the second experiment (345 mg/L start concentrrtion) after  35 days  more as 60 % degradation was  noticed. Combining the results from both experiments, it can be concluded that Reaction mass of disodium 2,2’-oxydiethanesulfonate and sodium ethenesulfonate degrades rapidly and is clearly inherently biodegradable.Furthermore, then test material may qualify to be labelled as readily biodegradable based on the results of the first experiment.

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable

Type of water:

freshwater