Registration Dossier
Registration Dossier
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EC number: 202-448-4 | CAS number: 95-76-1
Long-term toxicity to fish
Administrative data
- Endpoint:
- fish, juvenile growth test
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP Guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 998
- Report date:
- 1998
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 3,4-dichloroaniline
- EC Number:
- 202-448-4
- EC Name:
- 3,4-dichloroaniline
- Cas Number:
- 95-76-1
- Molecular formula:
- C6H5Cl2N
- IUPAC Name:
- 3,4-dichloroaniline
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebrafish
- Strain: Hamilton Buchanan
- Source: Bio International B.V. (nederlands)
- Weight at study initiation: 1.0-3.0 cm
FEEDING DURING TEST
- Food type: Tetra Min fish food
- Amount: 2% fish weight/day
- Frequency: 3 times a day
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 14 d
Test conditions
- Hardness:
- 14-14.3 °dH
- Test temperature:
- 22 +- 1°C
- pH:
- ca. 8 +- 1 °C
- Dissolved oxygen:
- ca 90-100 % saturation
- Nominal and measured concentrations:
- Nominal concentrations: 0.05, 0.16, 0.5, 1.6, 5.0 mg/L. Additionally, a control was tested
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- size, fill volume: 300*135*200 mm, 5 L
- Aeration: yes
- Renewal rate of test solution (frequency/flow rate): 3 times a week
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to ISO
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16/8 light dark cycle
EFFECT PARAMETERS MEASURED : behaviour and mortality
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.18 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 14 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 3.7 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
