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EC number: 412-720-6 | CAS number: 156653-67-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 14 January 2002 to 17 January 2002
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Study conducted to EU & OECD test guidance in compliance with GLP. This study is read across to the structural analogue Reaktiv-Gelb F-97494, which is considered to be structurally equivalent to the substance to be registered. Details of structure are detailed below under "illustrations"
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 002
- Report date:
- 2002
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- The study was conducted according to the Commission Directive 88/302/EEC; Official Journal of the EC L 133 Part C (1988). This test method is in most parts identical with OECD Guideline 209.
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Details on test material:
- See below
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Melting point: > 300 deg C
- Boiling point: Not measured
- Vapour pressure: < 10E-05 hPa
- Water solubility (under test conditions): 108 g/l
- Solubility in organic solvents: Not measured.
- log Pow: < -4.9
- Adsorption characteristics:
- ready biodegradation: 8% after 28 days
OTHER PROPERTIES (if relevant for this endpoint)
- Results of test for ready biodegradability: 8% after 28-days in a manometric test
:
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- No sampling was conducted.
Test concentrations (mg/l): 100, 1000, 10000
* Test substance concentration in physico-chemical oxygen consumption control (mg/l): 10000
Type of application: direct weighing
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Two controls without test substance are included in the test design, one at the start and the other at the end of the test series. Each batch of activated sludge is checked using a reference substance.
Concentration reference substance (3.5-DichIorophenol mg/l): 5, 10, 20
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Type: mixed population of aquatic microorganisms (activated sludge)
Origin: aeration tank of a waste water treatment plant treating predominantly domestic sewage (Wupper area water authority)
Pretreatment: aeration of the activated sludge; daily feed with synthetic medium
pH of the suspension before application: 7.3
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Post exposure observation period:
- No post exposure observation period
Test conditions
- Hardness:
- No data
- Test temperature:
- 19.0 - 19.3 C
- pH:
- 8.2 - 8.4
- Dissolved oxygen:
- 5.3 - 6.3 mg/l - start
2.7 - 3.6 mg/l - end - Salinity:
- Not applicable.
- Nominal and measured concentrations:
- Test concentrations (mg/l): 100, 1000, 10000
* Test substance concentration in physico-chemical oxygen consumption control (mg/l): 10000 - Details on test conditions:
- Test concentration of the activated sludge : 520 mg/l suspended solids
Test temperature: 20 ± 2° C
Incubation time: 3 hours with permanent aeration
The physico-chemical oxygen consumption control is carried out, since some substances can also consume oxygen by chemical reactivity. In order to be able to tell the difference between physico-chemical oxygen consumption and biological oxygen consumption (respiration), at least the maximum concentration of the test substance is tested without activated sludge. - Reference substance (positive control):
- yes
- Remarks:
- 3.5-DichIorophenol
Results and discussion
Effect concentrations
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 000 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- Because of strong respiration of the activated sludge only 0 .52 g/l ss were used.
The test substance has been added to about 130 ml deionised water and stirred overnight before testing (equilibration phase).
Test concentrations are given as nominal concentrations and have not been confirmed by analytical methods.
The physico-chemical oxygen consumption is determined at 10000 mg/l lest substance concentration. No physico-chemical oxygen consumption has been determined. Therefore lower concentrations of the test substance cause no physico-chemical oxygen consumption (deduced values). - Results with reference substance (positive control):
- The reference substance concentration result from the quantities used in the preparations and have not been confirmed by analytical methods.
EC50 of the reference substance 3,5-dichlorophenol is in the range 5-30 mg/l - Reported statistics and error estimates:
- All validity criteria of the test method were met: -
respiratory rate of the 2 controls differs less than 15 %
respiratory rate of the controls < 60 mg 02 /I . h
Any other information on results incl. tables
Measured values of test substance / control / reference substance
|
Test concentration [mg/l] |
O2start
[mg O2/l] |
O2end
[mg O2/l] |
Exposure time [min.] |
Temp.
[⁰C] |
pH |
Test substance |
100 |
5.7 |
2.7 |
5 |
19.2 |
8.4 |
1000 |
6.2 |
3.6 |
4 |
19.1 |
8.3 |
|
10000 |
5.3 |
2.9 |
4 |
19.1 |
8.2 |
|
Control 1 |
|
6.3 |
3.1 |
5 |
19.0 |
8.4 |
Control 2 |
|
6.2 |
2.9 |
5 |
19.3 |
8.3 |
Physico-chemical oxygen consumption control |
10000 |
8.8 |
8.8 |
9 |
19.2 |
7.3 |
Reference substance 3.5 -Dichlorophenol |
5 |
6.4 |
2.7 |
7 |
19.1 |
8.4 |
10 |
6.5 |
3.3 |
7 |
19.1 |
8.4 |
|
20 |
7.8 |
6.4 |
8 |
19.2 |
8.4 |
Test substance: Reaktivgelb F97-494
Test concentration
[mg/l] |
Respiratory rate test substance
[mg/l . h] |
Phys-chem. O2consumption
[mg/l . h] |
Respiratory rate - phys.-chem. O2consumption [mg/l . h] |
Inhibition
[%] |
100 |
36.0 |
0.0* |
36.0 |
7.7 |
1000 |
39.0 |
0.0* |
39.0 |
0.0 |
10000 |
36.0 |
0.0 |
36.0 |
7.7 |
Control, mean |
39.0 |
|
||
Control 1 |
38.4 |
|||
Control 2 |
39.6 |
* The physico-chemical oxygen consumption is determined at 10000mg/l test substance concentration. No physico-chemical oxygen consumption has been determined. Therefore lower concentrations of the test substance cause no physico-chemical oxygen consumption (deduced values).
Reference substance: 3.5-Dichlorophenol
Test concentration [mg/l] |
Respiratory rate [mg/l . h] |
Inhibition [%] |
5 |
31.7 |
18.7 |
10 |
27.4 |
29.7 |
20 |
10.5 |
73.1 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Reaktivgelb F97-494 showed 7.7 % respiration inhibition of activated sludge at a test substance concentration of 10000 mg/l.
EC50> 10000 mg/l - Executive summary:
This study is read across to the structural analogue Reaktiv-Gelb F97-494., which is considered to be structurally equivalent to the substance to be registered
A study was performed to assess the toxicity of Reaktivgelb F97-494 to bacteria.
The study was conducted in accordance with the Commission Directive 88/302/EEC; Official Journal of the EC L133 Part C (1988). Study performed in compliance with the Principles of Good Laboratory Practice (GLP).
The activated sludge was exposed to Reaktivgelb F97-494 at different concentrations. The respiration rate of each mixture was determined after aeration periods of 3 hours.
Test substance, by analogue of read across showed 7.7 % respiration inhibition of activated sludge at a test substance concentration of 10000 mg/l.
EC50>10000mg/l
The substance is not considered to be harmful to sludge micro-organisms.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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