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EC number: 226-603-0 | CAS number: 5435-64-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- GLP-Study according to OECD Guideline
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 2000
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- (1992)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- (1992)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 3,5,5-trimethylhexanal
- EC Number:
- 226-603-0
- EC Name:
- 3,5,5-trimethylhexanal
- Cas Number:
- 5435-64-3
- Molecular formula:
- C9H18O
- IUPAC Name:
- 3,5,5-trimethylhexanal
- Test material form:
- liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
- Details on test solutions:
- For the preparation of a stock solution 1 g/l of the test substance was equilibrated in synthetic fresh water without NaHCO3,
stirred for about 18 h, and finally filtered of. The resulting solution served as stock solution. NaHCO3 was added after sampling for analytical verification. The test solutions were prepared daily.
Test organisms
- Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- Cyprinus carpio (Common carp)
Source: Bio International B.V., Someren, Netherlands
Size: 3 - 5 cm
Maintenance: The fish are maintained in dechlorinated drinking water in 200 l tanks or in 300 l glass tanks at 20 ° C +/- 2 ° C with continuous flow.
The daily amount of feed (Alma) is administered in an amount of approximately 2 % of the body weight. The animals are held in quarantine for
14 days. Only then, they are used in tests.
The animals showed a normal behaviour pattern at the beginning of the test and were free from visible diseases.
The mortality 7 days before test began was < 5 %.
Pretreatment: The test animals were quarantined for 14 days prior to the start of the test without any treatment.
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- 14 ° dH
- Test temperature:
- mean = 19.8 ° C
mind. 19.5 ° C/max. 20.3 ° C - pH:
- 7.8 - 8.9
- Dissolved oxygen:
- 86 - 104 % saturation
- Nominal and measured concentrations:
- nominal and measured concentrations: 6 mg/L,11 mg/L, 20 mg/L, 34 mg/L, and 60 mg/L.
- Details on test conditions:
- Test tank: 20 l
Test volume: 10 l, changed daily
Aeration: continuous in the test tank
Light/dark cycle: 16/8 h
Density of animals: 10 animals per tank, each at ~ 0.91 g
Feed: none - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 45 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other:
- Remarks:
- range 34-60 mg/L
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 34 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other:
- Remarks:
- The highest concentration at which no mortality occured.
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 60 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other:
- Remarks:
- highest cocentration under the test conditions.
- Details on results:
- Analytical values of stability control, test solutions and stock solution. The concentrations are based on the substance. The measured test
concentrations after 0 h did not deviate by more than 20 % from the nominal concentrations. Additionally the measured concentration after 24 h
(first batch) did not deviate more than 20 % from the feshly prepared test solutions. Therefore it can be concluded that the test substance (or possible hydrolysis products) was present over the whole exposure time. The biological values were calculated with the nominal concentrations.
The highest concentration at which no mortality (< 10 %) after 96 h exposure occured was 96 h LC 0 = 34 mg/l.
The lowest concentration at which 100 % mortality after 96 h exposure occured, ws 96 LC 100 = 60 mg/l.
Any other information on results incl. tables
- Sublethal observations / clinical signs:
s. attached test report
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- 96 h LC50 = 45 mg/l (Concentration based on the substance)
- Executive summary:
Test substance 3,5,5 -Trimethylhexanal
Test organism: Cyprinus carpio
Test type : Acute toxicity 96h LC50 according to OECD 203
96 h LC50 = 45 mg/l
(Concentration based on the test substance)
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