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EC number: 614-295-4 | CAS number: 68131-40-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 28-29 April 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study conducted to accepted international guidelines and to GLP.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.6800 (Modified Activated Sludge, Respiration Inhibition Test for Sparingly Soluble Chemicals)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- yes
- Details on test solutions:
- Information provided by the Sponsor indicated that the test substance was a liquid and was insufficiently soluble in water to prepare a stock solution. Therefore at test initiation, concentrations of the test substance were achieved by the addition of appropriate volumes to 284 mL of reverse osmosis water. See table 1 in "Any other information on materials and methods" below.
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture:
- Method of cultivation:
- Preparation of inoculum for exposure: On the day of collection, an aliquot (10 mL) of the activated sludge was filtered through a dried and preweighed Whatman GF/C filter paper, which was then dried again at approximately 105°C for at least one hour, allowed to cool in a desiccator and reweighed. The mixed liquor suspended solids (MLSS) content of the activated sludge was then calculated and concentrated by the removal of supernatant. Synthetic sewage (50 mL/L) was added to the stock of activated sludge and this was aerated overnight. On the day of the test, the MLSS content of the sludge was determined (in triplicate) and adjusted to 4 g/L by the addition of tap water. The pH and temperature of the sludge were also measured. Aliquots (200 mL) were then added to each mixture to give a final MLSS concentration of 1.6 g/L.
- Pretreatment:
- Initial biomass concentration: - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- 10 minutes
- Test temperature:
- ca 20°C
- pH:
- 7.4-8.1
- Dissolved oxygen:
- See table 3 in results section.
- Nominal and measured concentrations:
- Nominal concentrations 0, 10, 30, 100, 300, 1000 mg/L
- Details on test conditions:
- See table 1 below for the preparation of the test mixtures.
The prepared mixtures were aerated for three hours in a thermostatically-controlled water bath, using a glass aerator connected to a laboratory supply of oil-free compressed air (one litre/minute). Following the exposure period, a well-mixed sample of each mixture was transferred to a biochemical oxygen demand (BOD) bottle (nominal capacity, 270 mL). The rate of oxygen consumption was measured, over a period of approximately 10 minutes or until the dissolved oxygen concentration fell below ca. 2 mg O2/L using a Yellow Springs Instrument (YSI) dissolved oxygen meter with a self stirring bottle probe connected to a chart recorder. The pH and temperature of the samples were measured at the start and end of the test. - Reference substance (positive control):
- yes
- Remarks:
- 3,5- Dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 824 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 95% CL was 355 to 2680 mg/L
- Duration:
- 72 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- 39 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 95% CL was 5.1 to 162 mg/L
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Relevant effect levels: 3-hour EC50 for 3,5-dichlorophenol was 6.9 mg/L. - Validity criteria fulfilled:
- yes
- Conclusions:
- The three-hour EC50 for Softanol 30 was calculated to be 824 mg/L (95% confidence limits, 355 to 2680 mg/L). The EC20 was calculated to be 39 mg/L (95% confidence limits, 5.1 to 162 mg/L). The EC80 could not be calculated.
Reference
Table 3 below gives the measurements of dissolved oxygen in the study.
Table 3 Dissolved oxygen (DO) concentrations, measurement times, respiration rates and percentage inhibition
Test mixture |
Initial DO concentration in the culture(mgO2/L) |
Initial measured DO concentration(mgO2/L) |
Final measured DO concentration(mgO2/L) |
Measurement time(minutes) |
Specific respiration rate(SRR mgO2/g/h) |
% inhibition |
Control (1) |
7.7 |
6.5 |
2.5 |
5.9 |
25.4 |
|
Softanol 30 (mg/L) |
|
|
|
|
|
|
10 |
7.4 |
6.0 |
2.0 |
6.1 |
25.6 |
0 |
30 |
6.9 |
6.0 |
2.0 |
6.9 |
21.7 |
12 |
100 |
7.9 |
7.0 |
3.7 |
8.1 |
15.3 |
38 |
300 |
7.6 |
7.0 |
4.0 |
8.1 |
13.9 |
44 |
1000 |
7.6 |
7.0 |
4.2 |
8.1 |
13.0 |
47 |
1000 |
7.9 |
7.0 |
4.5 |
7.3 |
12.8 |
48 |
1000 |
8.2 |
7.4 |
5.0 |
7.3 |
12.3 |
50 |
3,5-DCP (mg/L) |
|
|
|
|
|
|
3 |
8.7 |
8.0 |
4.0 |
8.1 |
18.5 |
25 |
10 |
9.4 |
8.7 |
6.7 |
7.8 |
9.6 |
61 |
32 |
9.8 |
9.5 |
8.7 |
8.7 |
3.4 |
86 |
Control (2) |
7.4 |
6.5 |
2.5 |
6.3 |
23.8 |
|
Description of key information
The effect of Softanol 30 on the respiration rate of activated sludge was assessed by the methods detailed in OECD Test Guideline 209, ‘Activated Sludge, Respiration Inhibition Test’ and EC Directive 88/302, Method C.11, ‘Biodegradation - Activated Sludge Respiration Inhibition Test’ (Huntingdon Life Sciences Ltd, 2010, PLZ0027)
Samples of activated sludge, fed with synthetic sewage, were exposed to dilutions of the test substance (concentrations of 10, 30, 100, 300 and 1000 mg/L. for three hours.
The three-hour EC50 for Softanol 30 was calculated to be 824 mg/L (95% confidence limits, 355 to 2680 mg/L). The EC20 was calculated to be 39 mg/L (95% confidence limits, 5.1 to 162 mg/L). The EC80 could not be calculated.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 824 mg/L
Additional information
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