Benzyldimethyl[2-[(1-oxoallyl)oxy]ethyl]ammonium chloride

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Adequacy of study:

other information

Study period:

21/11/1994-25/11/1994

Rationale for reliability incl. deficiencies:

other: Well documented report of a guideline study conducted to GLP.

Data source

Reference

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test material

Test material information

Constituent 1

Reference substance name:

Automatically generated during migration to IUCLID 6, no data available

IUPAC Name:

Automatically generated during migration to IUCLID 6, no data available

Details on test material:

- Name of test material (as cited in study report): ADAMQUAT BZ 80
- Physical state: Clear yellow liquid.
- Analytical purity: 80.4
- Impurities (identity and concentrations): water - 19.6%, EMHQ (stabiliser) - 337 ppm
- Composition of test material, percentage of components: 100%
- Purity test date: 28/07/1994
- Lot/batch No.: BZ 008
- Expiration date of the lot/batch: not specified
- Stability under test conditions: stable
- Storage condition of test material: at room temperature and away from light

Sampling and analysis

Details on sampling:

A sample of 4 ml was taken on 3 occasions during the test (at the beginning [T0]; just before changing the first solution [T 24]; at the end of the test [T 96]). As the test substance was found not to be toxic at the limit test concentration of 100 mg/l (maximum) the study was considered as complete and samples were analysed.

Test solutions

Details on test solutions:

DILUTION WATER: Filtered and dechlorinated potable water, which was then softened by a Permo water softening system. Half of the water passed directly into a holding tank attached to the flow through system while the remaining half first passed through a deioniser producing ultra-pure water, which was then fed into the holding tank. The test substance was dissolved in aqueous solution at 100mg/l, agitated mechanically for 24 hours in an attempt to completely dissolve the test substance. This stock solution was then used to prepare the test solutions directly.

Test organisms

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow Trout
- Source: "Labbas", Acquigny, 27400 Louviers, France
- Length at study initiation (length definition, mean, range and SD): The animals were an average of 47.7 mm long, ranging from 40 to 54 mm.
- Feeding during test: Animals were not fed during test.

ACCLIMATION
- Acclimation period: Animals were acclimated for 7 days in a flow-through system in water (same quality used in the test).
- Type and amount of food:not specified
- Feeding frequency: twice daily ceasing 24 hours before the start of the test
- Health during acclimation (any mortality observed): no adverse effects specified

Study design

Total exposure duration:

96 h

Test conditions

Hardness:

140±20 mg/l as CaCO3.

Test temperature:

13-17°C.

pH:

7.31-7.77

Dissolved oxygen:

Oxygen concentration: maintained at > 60%of the air saturation value at 13-17°C.

Nominal and measured concentrations:

Nominal concentrations: 0.1, 1, 10, 100 mg/l.

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: not specified
- Material, size, headspace, fill volume: not specified
- Aeration: not used
- No. of organisms per vessel: 5 fish per concentration at the following nominal concentrations: 0.1, 1, 10mg/l. 7 fish for the nominal concentration 100mg/l.
- No. of vessels per concentration (replicates): 1
- Biomass loading rate: The mean weight based on sample of 10 fish removed at random from the acclimatisation tank was 1.62g, equivalent to a loading of 0.76 g/l in the limit test.
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: : Filtered and dechlorinated potable water, which is then softened by a Permo water softening system. Half of the water passes directly into a holding tank attached to the flow through system while the remaining half first passes through a deioniser producing ultra-pure water, which is then fed into the holding tank.
- Total organic carbon: not specified
- Particulate matter: not specified
- Metals: not specified
- Pesticides: not specified
- Chlorine: not specified
- Alkalinity: not specified
- Ca/mg ratio: not specified
- Conductivity: not specified
- Intervals of water quality measurement: 24 hours
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: A light/dark cycle of 16/8 hours.
- Light intensity: not specified
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality or visible abnormalities.

Results and discussion

Effect concentrations

Duration:

96 h

Effect conc.:

100 mg/L

Details on results:

Mortality in the control and the limit test concentration of 100 mg/l was 0%.
No mortality or clinical signs were observed.

Applicant's summary and conclusion

Conclusions:

ADAMQUAT BZ was non-toxic to Onchorhynchus mykiss at 100 mg/l in a semi-static test system during a 96-hour exposure period. The LC0 was 100 mg/l (the highest test concentration).