Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 939-648-2 | CAS number: 75081-73-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Apr. - July 2021
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 022
- Report date:
- 2022
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.5100 - Bacterial Reverse Mutation Test (August 1998)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- tetrasodium 4-{2-[(Z)-(1-oxidododecylidene)amino]ethoxy}-4-oxo-2-sulfobutanoate 4-{2-[(Z)-(1-oxidododecylidene)amino]ethoxy}-4-oxo-3-sulfobutanoate
- EC Number:
- 939-648-2
- Cas Number:
- 75081-73-1
- Molecular formula:
- C18H31NNa2O8S
- IUPAC Name:
- tetrasodium 4-{2-[(Z)-(1-oxidododecylidene)amino]ethoxy}-4-oxo-2-sulfobutanoate 4-{2-[(Z)-(1-oxidododecylidene)amino]ethoxy}-4-oxo-3-sulfobutanoate
- Test material form:
- other: Coarse white pieces
Constituent 1
Method
- Target gene:
- Histidine (his- → his+)
Tryptophane (trp- → trp+)
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2
- Additional strain / cell type characteristics:
- other: TA98, TA1535 and E. coli are obtained from MOLTOX, INC., NC 28607, USA. Tester strains TA100 and TA1537 are obtained from Xenometrix AG, Switzerland. Stored as stock cultures with nutrient broth (OXOID)/DMSO (approx. 8% v/v) over liquid nitrogen.
- Metabolic activation:
- with and without
- Metabolic activation system:
- Type and composition of metabolic activation system:
- source of S9 :
The S9 liver microsomal fraction was prepared at Eurofins Munich and obtained from Trinova Biochem GmbH, Gießen, Germany.
- method of preparation of S9 mix:
Male Wistar rats are induced with phenobarbital (80 mg/kg bw) and β- naphthoflavone (100 mg/kg bw) for three consecutive days by oral route (Eurofins) or male Sprague Dawley rats are induced with phenobarbital/ β-naphthoflavone (Trinova).
A stock of the supernatant containing the microsomes is frozen in aliquots between 2 and 5 mL and stored at = -75 °C. The S9 mix preparation is performed according to Ames et al.
- concentration of S9 mix:
The protein concentration in the S9 preparation of Eurofins Munich was 34.4 mg/mL (Lot: 070521), and in the S9 preparation of Trinova, 37.8 mg/mL (Lot: 4423). The protein concentration of Lot No.: 4423 was adjusted to 30 mg/mL
- quality controls of S9 (e.g., enzymatic activity, sterility, metabolic capability):
at Eurofins Munich:
a) Biological activity in the Salmonella typhimurium assay using 2-aminoanthracene and benzo[a]pyrene
b) Sterility Test
at Trinova Biochem GmbH:
a) Alkoxyresorufin-O-dealkylase activities
b) Test for the presence of adventitious agents
Promutagen activation (including biological activity in the Salmonella typhimurium assay using 2-aminoanthracene and benzo[a]pyrene) - Test concentrations with justification for top dose:
- The toxicity of the test item was determined with tester strains TA98 and TA100 in a pre-experiment. Eight concentrations were tested for toxicity and mutation induction with each three plates:
0.00316, 0.0100, 0.0316, 0.100, 0.316, 0.100, 0.316, 1.0, 2.5 and 5.0 µL/plate.
The experimental conditions in this pre-experiment were the same as described below for the main experiment I (plate incorporation test).
As no precipitation nor limiting toxicity of the test item was observed in either tester strain used in the pre-experiment, 5.0 μL/plate was selected as the maximum concentration to be used in experiment I. - Vehicle / solvent:
- - Justification for choice of solvent/vehicle:
solubility
The test item was dissolved in A. dest. and diluted prior to treatment. The solvent was compatible with the survival of the bacteria and the S9 activity. A correction factor of 2.55 was applied to consider the active components of the test item.
Controls
- Untreated negative controls:
- yes
- Remarks:
- Aqua dest.
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- methylmethanesulfonate
- other: 4-NOPD, 4-nitro-o-phenylene-diamine, 10 µg/plate; 2-AA, 2-aminoanthracene, 2.5 µg/plate (S. thyphimurium) or 10 µg/plate (E. coli)
- Details on test system and experimental conditions:
- NUMBER OF REPLICATIONS:
- Number of cultures per concentration (single, duplicate, triplicate): Triplicate
- Number of independent experiments : 2, if first experiment negative or equivocal
METHOD OF TREATMENT/ EXPOSURE:
- Cell density at seeding (if applicable):
Samples of each tester strain are grown by culturing for 12 h at 37 °C in S. typhimurium medium (Nutrient Broth) and E. coli medium (Luria Bertani), respectively, to the late exponential or early stationary phase of growth (approx. 109 cells/mL).
- Test substance added:
1. experiment - in agar (plate incorporation)
2. experiment - preincubation
TREATMENT AND HARVEST SCHEDULE:
- Exposure duration/duration of treatment: 60 min.
- Incubation after exposure: 48 h at 37°C on selective medium
METHODS FOR MEASUREMENT OF CYTOTOXICITY
- Method: background growth inhibition - Rationale for test conditions:
- according to current OECD guideline
- Evaluation criteria:
- The mutation factor is calculated by dividing the mean value of the revertant counts by the mean values of the solvent control (the exact and not the rounded values are used for calculation).
A test item is considered as mutagenic if:
- a clear and dose-related increase in the number of revertants occurs and/or
- a biologically relevant positive response for at least one of the dose groups occurs
in at least one tester strain with or without metabolic activation.
A biologically relevant increase is described as follows:
- if in tester strains TA98, TA100 and E. coli the number of reversions is at least twice as high
- if in tester strains TA1535 and TA1537 the number of reversions is at least three times higher
as compared to the reversion rate of the solvent control.
A test item producing neither a dose related increase in the number of revertants nor a reproducible biologically relevant positive response at any of the dose groups is considered to be non-mutagenic in this system. - Statistics:
- According to the OECD guidelines, the biological relevance of the results is the criterion for the interpretation of results, a statistical evaluation of the results is not regarded as necessary.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- at concentrations of 0.316 μL/plate and higher (with and without metabolic activation)
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- at concentrations of 0.316 μL/plate and higher (with and without metabolic activation)
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- at concentrations of 0.316 μL/plate and higher (with and without metabolic activation)
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- at concentrations of 0.316 μL/plate and higher (with and without metabolic activation)
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- In tester strain E. coli WP2 uvrA (pKM101), toxic effects of the test item were noted at concentrations of 2.5 μL/plate and higher (without metabolic activation).
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
Any other information on results incl. tables
please refer to attachment
Applicant's summary and conclusion
- Conclusions:
- In conclusion, it can be stated that during the described mutagenicity test and under the experimental conditions reported, the test substance did not cause gene mutations by base pair changes or frameshifts in the genome of the tester strains used.
Therefore, the test item is considered to be non-mutagenic in this bacterial reverse mutation assay. - Executive summary:
The test item was investigated for its potential to induce gene mutations according to the plate incorporation test (experiment I) and the pre-incubation test (experiment II) using Salmonella typhimurium strains TA98, TA100, TA1535, TA1537 and tester strain E. coli WP2 uvrA (pKM101).
In two independent experiments several concentrations of the test item were used. Each assay was conducted with and without metabolic activation. The concentrations, including the controls, were tested in triplicate. The following concentrations of the test item were prepared and used in the experiments:Experiment I:
0.0316, 0.100, 0.316, 1.0, 2.5 and 5.0 μL/plate
Experiment II:
0.00316, 0.0100, 0.0316, 0.100, 0.316 and 1.0 μL/plate
(TA98, TA100, TA1535, TA1537)
0.0316, 0.100, 0.316, 1.0, 2.5 and 5.0 μL/plate
(E. coli WP2 uvrA (pKM101))No precipitation of the test item was observed in any tester strain used in experiment I and II (with and without metabolic activation).
The microbial contamination observed in one plate (experiment II, TA98, 0.0316 μL/plate, with metabolic activation) did not affect the quality or integrity of the results as the microbial contamination could be clearly distinguished from the Salmonella typhimurium revertants and thus did not affect the evaluation.
Toxic effects of the test item were observed in experiment I in tester strains TA98 and TA100 at concentrations of 2.5 μL/plate and higher (without metabolic activation). In tester strain TA1535, toxic effects of the test item were noted at concentrations of 1.0 μL/plate and higher (without metabolic activation) and at a concentration of 5.0 μL/plate (with metabolic activation). In tester strain TA1537 toxic effects of the test item were observed at a concentration of 5.0 μL/plate (without metabolic activation).
In experiment II, toxic effects of the test item were noted in tester strains TA98, TA100, TA1535 and TA1537 at concentrations of 0.316 μL/plate and higher (with and without metabolic activation). In tester strain E. coli WP2 uvrA (pKM101), toxic effects of the test item were noted at concentrations of 2.5 μL/plate and higher (without metabolic activation).
No biologically relevant increases in revertant colony numbers of any of the five tester strains were observed following treatment with the test substance at any concentration level, neither in the presence nor absence of metabolic activation in experiment I and II.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.