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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
in vivo mammalian germ cell study: cytogenicity / chromosome aberration
Remarks:
Type of genotoxicity: chromosome aberration
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
No test guideline followed, inadequate documentation, no indication of purity. A method was used that has largely been superseded by metaphase analysis, and that is difficult to perform and read. No dose related effect is presented, and no historical control data are presented to see if the effect is within normal statistical bounds for controls.

Data source

Reference
Reference Type:
publication
Title:
Long-term effects of N-methylpyridinium chloride on the target organism
Author:
Antonenko T, Nechkina M, Nakoryakova M, Kulikova L & Chomutova T
Year:
1984
Bibliographic source:
Gigiena i Sanitariya (1984), 49(1), 74-75 (publication written in Russian)

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
cytogenic analysis of spiral cord (anaphasic analysis, bone-marrow cells)
GLP compliance:
no
Type of assay:
unspecified

Test material

Constituent 1
Chemical structure
Reference substance name:
1-methylpyridinium chloride
EC Number:
231-658-9
EC Name:
1-methylpyridinium chloride
Cas Number:
7680-73-1
Molecular formula:
C6H8ClN
IUPAC Name:
1-methylpyridin-1-ium chloride
Details on test material:
analytical purity: no data

Test animals

Species:
rat
Strain:
other: white rats
Sex:
male
Details on test animals or test system and environmental conditions:
rats were sacrified by breaking the cervical spine

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
no data
Details on exposure:
The substance was administered into the stomach.
Frequency of treatment:
a) single treatment at 1/50 LD50
b) repeated treatment at 1/500 LD50 and 1/5000 LD50 over a period of 45 days
No. of animals per sex per dose:
no data
Control animals:
yes, concurrent no treatment
Positive control(s):
none

Examinations

Tissues and cell types examined:
cytogenic analysis of spiral cord (anaphasic analysis, bone-marrow cells)

Results and discussion

Additional information on results:
Cytogenic effects were reported. A single administration of 1/50 LD50 of the substance caused chromosome aberrations in the bone-marrow cells (primarily chromatid type). Repeated administration at 1/5000 LD50 showed chromosome and chromatid type aberrations.

Applicant's summary and conclusion

Executive summary:

There was some evidence for potential clastogenic and mitotic effects in vivo. However the data are of limited reliability. Without any result from a proper guideline study no conclusion can be drawn.