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EC number: 217-617-8 | CAS number: 1912-24-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 01 October 1986 to 13 November 1986
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Study design states it complies and it follows OECD guideline 471. Contains sufficient detail to suggest GLP-like characteristics even though no statement of certification is reported (reasonably thorough description of authors, dates, design, results, and interpretation).
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 986
- Report date:
- 1986
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- the data have not been statistically analysed
- Qualifier:
- according to guideline
- Guideline:
- other: Literatures ( see Principles of method)
- Deviations:
- not specified
- Principles of method if other than guideline:
- 1. AMES, B.N., F.D. LEE and W.E. DURSTON (1973), An Improved Bacterial Pest System for the Detection and Classification of Mutagens and Carcinogens. Proc. Natl. Acad. Sci. USA 70, 782786.
2. AMES, B.N., W.E. DURSTON, E. YAMASAKI and F.D. LEE (1973) ,Car cinogens are Mutagens: A Simple Test System Combining Liver Homogenates for Activation and Bacteria for Detection. Proc. Natl. Acad. Sci. USA 70, 22812285.
3. AMES, B.N., J. McCANN, and E: YAMASAKI (1975), Methods for Detecting Carcinogens and Mutagens with the Salmonella/Mammalian Microsome Mutagenicity Test. Mutation Res. 31, 347364. - GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Atrazine
- EC Number:
- 217-617-8
- EC Name:
- Atrazine
- Cas Number:
- 1912-24-9
- Molecular formula:
- C8H14ClN5
- IUPAC Name:
- 6-chloro-N2-ethyl-N4-(propan-2-yl)-1,3,5-triazine-2,4-diamine
- Details on test material:
- Batch No. lot. 210200
Purity: 98.2%
Constituent 1
Method
- Target gene:
- bacteria
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Additional strain / cell type characteristics:
- other: histidine-auxotrophic mutants
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 fraction of liver from rats induced with Aroclor 1254
- Test concentrations with justification for top dose:
- the test were performed with the following concentrations of the trial substance with and without microsomal activation: 20, 78, 313, 1250 and 5000 µg/0.1 ml.
- Vehicle / solvent:
- Dimethylsulfoxide (DMSO)
Controlsopen allclose all
- Untreated negative controls:
- yes
- Remarks:
- DMSO alone has been used as negative controls
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- 4-nitroquinoline-N-oxide
- Remarks:
- for the strain TA 100
Migrated to IUCLID6: 0.125 and 0.25 µg/0.1 ml phosphate buffer
- Untreated negative controls:
- yes
- Remarks:
- DMSO alone has been used as negative controls
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: daunorubicin-HCl, 5 and 10 µg/0.1 ml phosphate buffer
- Remarks:
- for the strain TA 98
- Untreated negative controls:
- yes
- Remarks:
- DMSO alone has been used as negative controls
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- for the strain 1535
Migrated to IUCLID6: 2.5 and 5 µg/0.1 ml bidistilled water
- Untreated negative controls:
- yes
- Remarks:
- DMSO alone has been used as negative controls
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: 9(5)-aminoacridine hydrochloride monohydrate, 50 and 100 µg/0.1 ml DMSO
- Remarks:
- for strains TA 98, TA 100 and TA 1537
- Untreated negative controls:
- yes
- Remarks:
- DMSO alone has been used as negative controls
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- cyclophosphamide
- Remarks:
- for the strain TA 1535
Migrated to IUCLID6: 250 µg/0.1 ml bidistilled water
- Details on test system and experimental conditions:
- In the experiments without and with the addition of microsomal activation mixture three Petri dishes are prepared per strain and per group (i.e. per concentration or per control group).
The plates are incubated for about 48 hours at 37 ± 1.5°C in darkness. - Evaluation criteria:
- Assay acceptance criteria:
A test is considered acceptable if the mean colony counts of the control values of all strains are within the acceptable ranges indicated in table 9 and if the results of the positive controls meet the criteria for a positive response.
In either case the final decision has to be based on scientific judgement. - Statistics:
- Criteria for a positive response
The test substance is considered to be positive in this test system if one or both of the following conditions are met:
a reproducible doubling of the mean number of revertants per plate above that of the negative control at any concentration level for one or more of the following strains: TA 98, TA 1535 and TA 1537,
a reproducible increase of the mean number of revertants per plate for any concentration above that of the negative control by a factor of l.5 for strain TA 100.
Generally a concentration-related effect should be demonstrable.
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- In the toxicity tests treatment of the bacteria with the highest concentration (5000 µg/0.l ml) of Atrazine tech. did not lead to a growth-inhibiting effect. This concentration was therefore selected as the highest in the mutagenicity tests.
In the experiments performed without and with microsomal activation, treatment with Atrazine tech. did not lead to an increase in the incidence of histidine-prototrophic mutants in comparison with the negative control.
Owing to a growth-inhibiting effect of the test substance in the experiments without and with microsomal activation, the number of back-mutants was reduced at the highest concentration. - Remarks on result:
- other: other: all strains
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
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