Registration Dossier
Registration Dossier
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EC number: 229-662-0 | CAS number: 6642-31-5
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study. Scientifically acceptable.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Test organisms (species):
- activated sludge
- Details on inoculum:
- Municipal activated sludge from laboratory wastewater treatment plant fed with municipal sewage. Concentration of dry substance = 1 g/l
The inoculum was washed and brought to a concentration of 5 g/l dry substance, then aerated during the night. 50 ml were added to a total volume of 250 ml to obtain a concentration of 1 g/l dry substance in the test. - Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 30 min
- Test temperature:
- 20 +/- 2 °C
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer-vessel (nominal volume 250 ml)
- Fill volume: 250 ml
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: 8 ml/vessel 100-fold concentrated OECD medium - Duration:
- 30 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
Reference
Description of key information
Toxicity to mircroorganisms: EC20, EC50 > 1000 mg/l after 30 min for municipal activated sludge (inhibition of total respiration / respiration rate, OECD 209, GLP)
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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