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Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)

Data source

Reference
Reference Type:
publication
Title:
Mutagenic and cell transforming activities of 1-chlor-2,4-dinitrobenzene and squaric acid dibutylester
Author:
Strobel, R.; Röhrborn, G.
Year:
1980
Bibliographic source:
Arch. Toxicol. 45: 307 - 314 (1980)

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 471 (Bacterial Reverse Mutation Assay)
GLP compliance:
not specified
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
1-chloro-2,4-dinitrobenzene
EC Number:
202-551-4
EC Name:
1-chloro-2,4-dinitrobenzene
Cas Number:
97-00-7
Molecular formula:
C6H3ClN2O4
IUPAC Name:
1-chloro-2,4-dinitrobenzene
Details on test material:
no data

Method

Species / strain
Species / strain / cell type:
other: TA 98, TA100, TA 1535, TA 1537, TA 1538
Metabolic activation:
with and without
Metabolic activation system:
S9-mix from rats
Test concentrations with justification for top dose:
see table below
Vehicle / solvent:
acetone

Results and discussion

Test resultsopen allclose all
Species / strain:
S. typhimurium TA 98
Metabolic activation:
with and without
Genotoxicity:
positive
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Untreated negative controls validity:
valid
Positive controls validity:
valid
Species / strain:
S. typhimurium TA 100
Metabolic activation:
with and without
Genotoxicity:
positive
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Untreated negative controls validity:
valid
Positive controls validity:
valid
Species / strain:
S. typhimurium TA 1535
Metabolic activation:
with and without
Genotoxicity:
ambiguous
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Untreated negative controls validity:
valid
Positive controls validity:
valid
Species / strain:
S. typhimurium TA 1537
Metabolic activation:
with and without
Genotoxicity:
ambiguous
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Untreated negative controls validity:
valid
Positive controls validity:
valid
Species / strain:
S. typhimurium TA 1538
Metabolic activation:
with and without
Genotoxicity:
positive
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Untreated negative controls validity:
valid
Positive controls validity:
valid

Any other information on results incl. tables

Results

 Strain Dose (µg/plate)  Mutants per plate  relative increase  S9
 TA 100 0   150  1  +
   6,3  134  0,9  +
   12,5  139  0,9  +
   25  143  1  +
   50  241  1,6  +
   100  1080  7,2  +
   0  148  1  -
   1  166  1,1  -
   10  332  5,4  -
   100  >2000    -
 TA 1535  0  12  1  +
   50  15  1,3  +
   100  18  1,5  +
   200  27    +
   0 24  1   -
   1  29  1,2  -
   10  27 1,1   -
   100  31    -
 TA 98 0   39  1 +
   6,3  41  1  +
   12,5  44  1,1  +
   25  38  1  +
   50  52  1,3  +
   100  193  5  +
   0  28  1  -
   1  30  1,1  -
   10  81  2,9  -
   100  559    -
 TA 1538  0  29  1  +
   25  36  1,3  +
   50  54  1,9  +
   100  127  4,4  +
   0  11  1  -
   1  15  1,4  -
   10  63  6  -
   100  491    -
 TA 1537  0  15  1  +
   50  12  0,8  +
   100  21  1,4  +
   200  42  2,8  +
   0  23  1  -
   1  35  1,5  -
   10  19  0,8  -
   100  33    -
     
         
         
         
         

Applicant's summary and conclusion

Conclusions:
DNCB caused mutagenic effects in the Salmonella/ microsome test with and without a metabolic activation system.