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EC number: 444-860-9 | CAS number: 474510-57-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Bioaccumulation: aquatic / sediment
Administrative data
Link to relevant study record(s)
- Endpoint:
- bioaccumulation in aquatic species: fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2005-03-03 to 2005-03-10
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)
- Version / remarks:
- adopted on June 14, 1996
- Deviations:
- no
- GLP compliance:
- yes
- Radiolabelling:
- no
- Details on sampling:
- - Sampling intervals/frequency for test organisms: on day 7, 14, 21, 23, 28
- Sampling intervals/frequency for test medium samples: on day 7, 14, 21, 23, 28 - Vehicle:
- yes
- Details on preparation of test solutions, spiked fish food or sediment:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was dissolved in DMSO to prepare 25000 mg/L test substance stock solution. The stock solution was diluted with the test water supply to be used for 96hr-LC50 test.
- Controls: yes
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): DMSO - Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- TEST ORGANISM
- Common name: common carp (cyprinus carpio)
- Source: Aqua stage 21 Tokyo shop (1-12-8, Ojihoncho, Kita-ku, Tokyo)
- Length at study initiation (lenght definition, mean, range and SD): 5.7 ± 0.3 cm
- Weight at study initiation (mean and range, SD): 2.03 ± 0.33 g
- Feeding during test: yes; no feeding a day before sampling
- Food type: Mixed carp pellets (Suimmy) (Crude protein 37.0% or greater; Crude fat 2.5% or greater)
- Amount: ca. 2% per body weight/day
- Frequency: daily
ACCLIMATION
- Acclimation period: 7 days
- Acclimation conditions (same as test or not): yes
- Health during acclimation (any mortality observed): no mortality was observed - Route of exposure:
- aqueous
- Test type:
- flow-through
- Water / sediment media type:
- natural water: freshwater
- Total exposure / uptake duration:
- 28 d
- Hardness:
- 43 mg/L
- Test temperature:
- 23.7 ± 0.6°C
- pH:
- 7.1 ±0.1
- Dissolved oxygen:
- 7.2 + 0.2 mg/L
- TOC:
- < 2 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass aquarium volume 100 L (water volume 50 L)
- Aeration: yes
- Renewal rate of test solution: 300 mL/min (432 L/day)
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 4
- No. of vessels per control / vehicle control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: JIS K0102 33.1 (alpha-Tolidine colorimetry method).
- Metals: copper < 0.005 mg/L
- Chlorine: < 0.01 mg/L
- Holding medium different from test medium: no
- Intervals of water quality measurement: temperature, pH and DO : on day 0, 7, 14, 21, 23, 28
OTHER TEST CONDITIONS
- Photoperiod: The operations were performed in a dark place because of instability of the test substance against the light.
RANGE-FINDING / PRELIMINARY STUDY
- Test concentrations: The 96hr-LC50 value for zebra-fish {Danio rerio) was determined to be 1.95 mg/L. Based on this value and the quantifiable limit of this analytical method, the following test concentrations were selected by setting safety factors of 1/100 and 1/1000. - Nominal and measured concentrations:
- - two nominal concentrations: 0.02 mg/L; 0.002 mg/L
- Reference substance (positive control):
- no
- Details on estimation of bioconcentration:
- BASIS INFORMATION
- Measured/calculated logPow: 2.3 - Conc. / dose:
- 0.02 mg/L
- Temp.:
- 23.7 °C
- pH:
- 7.1
- Type:
- BCF
- Value:
- < 1 dimensionless
- Basis:
- not specified
- Time of plateau:
- 21 d
- Calculation basis:
- steady state
- Conc. / dose:
- 0.002 mg/L
- Temp.:
- 23.7 °C
- pH:
- 7.1
- Type:
- BCF
- Value:
- < 10 dimensionless
- Basis:
- not specified
- Time of plateau:
- 21 d
- Calculation basis:
- steady state
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test substance does not accumulate in organisms.
- Executive summary:
In this study conducted to generally accepted scientific standards the test material (EC 444-860-9) was determined to not not bioaccumulate in organisms.
Reference
A bioconcentration study of the test item in carp, Cyprinus carpio, was conducted in compliance with the Test Methods Concerning a New Chemical Substance (Kashinhou). Based on a 96hr-LC50 of the test substance for zebra-fish (Danio rerio) (1.95 mg/L), two concentration levels were selected: 0.02 mg/L as a high exposure level and 0.002 mg/L as a low exposure level.
The test substance is a reaction product consisting of the main component (composition ratio 93.0%) and its isomer (1.1%), and three minor components (less than 1% for each component).
As the results of this study, the residual concentrations of p,p-isomer and m,p-isomer in fish were the detectable Umit or less, and the exposure period therefore was terminated after 28 days of exposure when the steady-states of these components were assumed to have been reached.
The bioconcentration factors (BCFs) of p,p-isomer and m,p-isomer in fish were 1 fold or less and 2 folds or less, respectively, at the high exposure level (0.02 mg/L group) and 10 folds or less and 20 folds or less, respectively, at the low exposure level (0.002 mg/L group).
Description of key information
The test substance does not accumulate in organisms. The result of a bioaccumulation study with Cyprinus carpio following the procedures of the OECD guideline 305C showed a BCF of <10 for a test concentration of 0.002 mg/L (Institute of Ecotoxicology Co., 2005). Even though DMSO was used as solubilizer, the study is regarded as valid since both test concentrations (0.02 and 0.002 mg/L) are well below the water solubility of 9.9 mg/L of the test substance.
Key value for chemical safety assessment
Additional information
Study conducted to generally recognised scientific standards with GLP.
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