3,3'-iminodi(propylamine)

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well-documented publication which meets basic scientific principles.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1978

Report date:

1978

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

3,3'-iminodi(propylamine)

EC Number:

200-261-2

EC Name:

3,3'-iminodi(propylamine)

Cas Number:

56-18-8

Molecular formula:

C6H17N3

IUPAC Name:

bis(3-aminopropyl)amine

Details on test material:

- Analytical purity: 99%

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Firma WIGA (Sulzfeld)
- Weight at study initiation: male animals 140g, female animals 130g
- all animals are healthy with unharmed skin

Administration / exposure

Type of coverage:

occlusive

Vehicle:

olive oil

Details on dermal exposure:

TEST SITE
- Area of exposure: 5 cm2
- Type of wrap if used: aluminium foil fixed with stickytape

REMOVAL OF TEST SUBSTANCE
- Washing: with warm water or water/lutrol mixture
- Time after start of exposure: 24 hours

TEST MATERIAL
- Amount(s) applied: 200, 400 and 640 mg/kg
- Concentration: 25% and 50%

Duration of exposure:

24 h

Doses:

200, 400 and 640 mg/kg

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 21 days
- Frequency of observations and weighing: 1 h, 1, 2, 8 , 14 and 21 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

200 - 400 mg/kg bw

Based on:

test mat.

Mortality:

200 mg/kg; none of the animals died after 14 days
400 mg/kg; 3/5 males and 5/5 females after 21 days
640 mg/kg: all animals died after 24 h

Clinical signs:

other: Apathy, 24 hours after application necrosis was observed on all animals

Gross pathology:

Diseased animals
400-640 mg/kg: dilation and acute congestion hyperaemia of the heart, obvious almost infarctious bloody lungs
640 mg/kg: bright kidneys

Euthanized animals
No abnormal findings

Applicant's summary and conclusion

Executive summary:

In a dermal toxicity study, comparable to OECD 402, dipropylenetriamine was applied in olive oil occlusively at single doses of 200 to 640 mg/kg to Sprague-Dawley rats (5/sex/dose).

The LD50 falls between 200 and 400 mg/kg bw.