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EC number: 229-129-2 | CAS number: 6416-66-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From August 31 to November 15, 1994
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Composite samples of each test concentration were drawn by mixing identical volumes of the test solutions taken from the approximate center of the test vessels. They were taken immediately before exposure and after 48 hours exposure and kept at - 18°C to - 22°C until analysis.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- - Stain: Stauss 1820- Source: CIBA-GEYGY- Feeding: none during the test- Breeding: Cultures of daphnia are maintained in glass vessels containing approx. 2.5 1 of reconstituted water at 20 ± 1 °C (water is renewed partially thrice weekly). At each renewal the daphnia are fed with a suspension of green algae (Scenedesmus subspicatus) supplemented by a suspension of TETRAMIN-extract in such quantities that the food is consumed after 24h.
- Water media type:
- freshwater
- Total exposure duration:
- 48 h
- Hardness:
- Reconstituted water containing65 mg NaHC03294 mg CaCl2 • 2 H20123 mg MgS04 • 7 H206 mg KClin 1000 ml bidistilled water.Total hardness : 240 mg CaC03/l.
- Test temperature:
- 20 ± 1°C (measured after 24 and 48 h)
- pH:
- 7.8 (measured after 24 and 48 h)
- Dissolved oxygen:
- The water was aerated with clean air for at least 24 hours before use.96% (measured after 24 and 48 h)
- Nominal and measured concentrations:
- - STOCK SOLUTION: 200.5 mg test substance were mixed and made up to 2000 ml with water.- NOMINAL: 2.0,4.4, 9.6,21,46 and 100 mg test substance- BLANK: water
- Details on test conditions:
- TEST SYSTEM- No. of organisms per vessel: 20 per concentration and control- No. of vessels per concentration (replicates): 4 replicates of 5 daphnia each- Pre-treatment: 24 hours before the begin of the exposure reproductive daphnia are separated from the young by sieving all individuals through a 800 um sieve. This operation is repeated immediately before the exposure and the young (0-24h of age) are retained for the test.OTHER TEST CONDITIONS- Lighting: Fluorescent light, 16 hours daily approx. 1500 lux 48OBSERVATIONImmobilizations were recorded after 24 and 48 hours exposure
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Remarks on result:
- other: 95% confidence limit
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 104 mg/L
- Nominal / measured:
- nominal
- Remarks on result:
- other: 95% confidence limit
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 46 mg/L
- Nominal / measured:
- nominal
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 46 mg/L
- Nominal / measured:
- nominal
- Results with reference substance (positive control):
- - Immobilization in blank: 5%
- Reported statistics and error estimates:
- The LC-50 values were calculated according to BERKSON, JASA 48 (1953), 569-599.EC-values were graphically determined on gausso-logarithmic probability paper
- Conclusions:
- EC 50 (48 h) calculated: 104 mg/L95 % confidence limit: 88-153 mg/LEC 50 (24 h) calculated: > 100 mg/LNOEC (48 h): 46 mg/LEC 0 (48 h): 46 mg/LEC 100 (48h): > 100 mg/L
- Executive summary:
The determination on ecotoxicity of the substance was determined in an Acute toxicity for Daphnia, test according to the guideline 92/69/EEC C.2. The test has been performed in compliance with the Procedures and Principles of Good Laboratory Practice. The test substance was tested on Daphnia magna Straus 1820 for 48 hours.
20 daphnids (0-24 hours old) were exposed to nominal concentrations of 2.0, 4.4, 9.6,21,46 and 100 mg test substance respectively.
Results are:
EC 50 (48 h) calculated: 104 mg/L
95 % confidence limit: 88-153 mg/L
EC 50 (24 h) calculated: > 100 mg/L
NOEC (48 h): 46 mg/L
EC 0 (48 h): 46 mg/L
EC 100 (48h): > 100 mg/L
Reference
Description of key information
EC 50 (48 h) calculated: 104 mg/l
EC 50 (24 h) calculated: > 100 mg/l
NOEC (48 h): 46 mg/l
EC 0 (48 h): 46 mg/l
EC 100 (48h): > 100 mg/l
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 104 mg/L
Additional information
The determination on ecotoxicity of the substance to be registered was determined in an acute test on Daphnia, test according to the guideline 92/69/EEC C.2. The test has been performed in compliance with the Procedures and Principles of Good Laboratory Practice. The test substance was tested on Daphnia magna Straus 1820 for 48 hours.
20 daphnids (0-24 hours old) were exposed to nominal concentrations of 2.0, 4.4, 9.6,21,46 and 100 mg test substance respectively.
Results are:
EC 50 (48 h) calculated: 104 mg/L
95 % confidence limit: 88-153 mg/L
EC 50 (24 h) calculated: > 100 mg/L
NOEC (48 h): 46 mg/L
EC 0 (48 h): 46 mg/L
EC 100 (48h): > 100 mg/L
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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