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EC number: 695-745-7 | CAS number: 1079221-49-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 10-Jan-2011 to 03-Feb-2011
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study conducted in compliance with international guidelines.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
- Report date:
- 2011
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2-({3-aminopyrazolo[1,5-a]pyridin-2-yl}oxy)ethan-1-ol hydrochloride
- EC Number:
- 695-745-7
- Cas Number:
- 1079221-49-0
- Molecular formula:
- C9 H11 N3 O2, ClH
- IUPAC Name:
- 2-({3-aminopyrazolo[1,5-a]pyridin-2-yl}oxy)ethan-1-ol hydrochloride
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: For the determination of the actual test item concentrations, duplicate samples were taken from each treatment at the test start and end of each test medium renewal period. The nominal test item concentrations of 1.1, 2.3, 5.0, 11, 23 and 50 mg/L were tested.
- Sampling method:Immediately after sampling, sodium dithionite solution with a nominal concentration of 10 mg/mL was added to each sample (100 μL per 10 mL of sample volume) in order to stabilize the latter for measurement. The samples were analyzed immediately after sampling.
- Sample storage conditions before analysis: no storage
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: At the start of each test medium renewal period, a stock solution with a nominal concentration of 100 mg/L was prepared by completely dissolving 100 mg (dosed amounts: 100.0 and 100.4 mg) of test item in 1000 mL of test water using intense stirring for 3 minutes at room temperature in the dark. This intensively mixed stock solution was used in a series of dilutions to prepare the test media of all test item concentrations. The test media were prepared just before introduction of the daphnids (i.e., start of the test and prior to the test medium renewal). As the test item is photodegradable, the preparation of the test media was performed as far as possible in the dark.
- Eluate:/
- Differential loading:/
- Controls: no special treatment
- Chemical name of vehicle (organic solvent, emulsifier or dispersant):no vehicle
- Concentration of vehicle in test medium (stock solution and final test solution):/
- Evidence of undissolved material (e.g. precipitate, surface film, etc): No remarkable observations were made concerning the appearance of the test media. All test media were clear solutions throughout the test period. At the three highest test concentrations (nominal 11 to 50 mg/L), the test media were already colored (purple) at the beginning of the test medium renewal periods. At the end of the renewal periods, the test media of the nominal concentrations of 2.3 to 50 mg/L were colored, at the three highest concentrations the intensity of the coloration had increased to dark purple.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain: Straus
- Source: originally supplied by the University of Sheffield / UK in 1992.
- Age at study initiation (mean and range, SD): At the start of the test, the organisms used in the test were 6-24 hours old and were not first brood progeny.
- Weight at study initiation (mean and range, SD):/
- Length at study initiation (length definition, mean, range and SD):
- Valve height at study initiation, for shell deposition study (mean and range, SD):/
- Peripheral shell growth removed prior to test initiation:/
- Method of breeding: Since 1992, the clone has been bred at Harlan Laboratories in reconstituted water of the quality identical to the water quality used in the tests (in respect to pH, main ions, and total hardness) and under temperature and light conditions identical to those of the tests. During breeding, daphnids are generally fed three times a week with an algal suspension of the green algae Desmodesmus subspicatus CHODAT, Strain No. 86.81 SAG, supplied by the Collection of Algal Cultures (SAG, Institute for Plant Physiology, University of Göttingen, 37073 Göttingen / Germany) and cultivated at Harlan Laboratories under standardized conditions or a mixture of this algal suspension and a commercial fish diet (Tetra Min® Hauptfutter, supplied by TETRA-Werke, 49304 Melle / Germany).
- Feeding during test: no
ACCLIMATION /
QUARANTINE /
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 250 mg/l as CaCO3
- Test temperature:
- The water temperature was maintained at 20-21 °C.
- pH:
- In consultation with the Sponsor, the pH of the test water was lowered to 6.0 (using 1 M hydrochloric acid) to increase the stability of the test item in the test water. At the beginning of the test medium renewal periods, pH values ranged between 6.1 and 6.4 after adjustment. At the end of the test medium renewal periods, the pH values of the test media were between 6.5 and 7.0.
- Dissolved oxygen:
- The dissolved oxygen concentrations in the test media and control were at least 8.2 mg/L.
- Salinity:
- /
- Nominal and measured concentrations:
- Nominal concentrations: 1.1, 2.3, 5.0, 11, 23 and 50 mg/L.
Measured concentrations (calculated as the arithmetic mean of the two geometric means of the concentrations measured at the start and end of the test medium renewal periods): 0.36 *, 0.75*, 1.9, 3.8, 8.0, 17 mg/L (*½ LOQ (0.128 mg/L) was used for the calculation of the mean measured concentration if the concentration at the end of the renewal period was- Details on test conditions:
- TEST SYSTEM
- Test vessel,Type (open / closed), Material, size, headspace, fill volume: 250-mL glass beakers filled with 200 mL of test medium
- Aeration: The test water was aerated prior to the start of the study until oxygen saturation was reached. During the test period, the test water was not aerated.
- Type of flow-through (e.g. peristaltic or proportional diluter):/
- Renewal rate of test solution (frequency/flow rate):every 24H
- No. of organisms per vessel:5
- No. of vessels per concentration (replicates):4
- No. of vessels per control (replicates):4
- No. of vessels per vehicle control (replicates):/
- Biomass loading rate:1 daphnia per 40 mL
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted test water according to ISO 6341 was used in the study. It consisted of analytical grade salts dissolved in purified water
- Total organic carbon:/
- Particulate matter:/
- Metals:/
- Pesticides:/
- Chlorine:/
- Alkalinity: 0.8 mmol/L
- Ca/mg ratio: 4:1
- Conductivity:/
- Culture medium different from test medium:No
- Intervals of water quality measurement: At the start and end of each test medium renewal period, the pH values, dissolved oxygen concentrations and water temperature were determined in each treatment. The appearance of the test media was visually recorded at the same time.
OTHER TEST CONDITIONS
- Adjustment of pH:In consultation with the Sponsor, the pH of the test water was lowered to 6.0 (using 1 M hydrochloric acid) to increase the stability of the test item in the test water.
- Photoperiod:The test was performed as far as possible in the dark to avoid a photolytic degradation of the test item.
- Light intensity:/
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : The immobility of the daphnids was determined by visual inspection after 24 and 48 hours of exposure. Those daphnids not able to swim within 15 seconds after gentle agitation of the test vessel were considered to be immobilized.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: between 2.09 and 2.2
- Justification for using less concentrations than requested by guideline:/
- Range finding study not reported in the report (not GLP), but results used for selection of the concentrations of the definitive study.- Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 3.8 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% Confidence interval
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 3.8 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CI
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 1.9 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.75
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 17 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities:
- Observations on body length and weight:/
- Other biological observations:No other symptoms than immobility were observed.
- Mortality of control: No
- Other adverse effects control:No
- Abnormal responses:At the mean measured test concentration of 1.9 mg/L, 10 daphnids were trapped at the water surface.
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values:/
- Effect concentrations exceeding solubility of substance in test medium:/ - Results with reference substance (positive control):
- - Results with reference substance : The result of the latest positive control test in October 2010 (48-hour EC50: 0.56 mg/L, study C97643) showed that the sensitivity of the test organisms was within the internal historical range (48-hour EC50 from 2000 to 2010: 0.43-1.1 mg/L).
Any other information on results incl. tables
Nominal test item concentration (mg/L) |
Mean initially measured concentration (mg/L) |
No. of daphnids tested |
Immobilized daphnids after 24 hours |
Immobilized daphnids after 48 hours |
||
|
|
|
No. |
% |
No. |
% |
Control |
--- |
20 |
0 |
0 |
0 |
0 |
1,1 |
0,36 |
20 |
0 |
0 |
0 |
0 |
2,3 |
0,75 |
20 |
0 |
0 |
0 |
0 |
5 |
1,9 |
20 |
0 |
0 |
0 (10*) |
0 |
11 |
3,8 |
20 |
13 |
65 |
13 (1*) |
65 |
23 |
8 |
20 |
18 |
90 |
18 |
90 |
50 |
17 |
20 |
20 |
100 |
20 |
100 |
*Value in parenthesis: number of test animals with symptoms of toxicity (daphnids trapped at the water surface)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- in the control no daphnids showed immobilization or other signs of disease or stress; the dissolved oxygen concentration at the end of the test was ≥3 mg/L in the control and test vessels
- Executive summary:
The acute toxicity of the test item to Daphnia magna was determined in a 48-hour semi-static test according to the EU Commission Directive 92/69/EEC, Part C.2, the Commission Regulation (EC) No. 440/2008, Part C.2 and the OECD Guideline for Testing of Chemicals, No. 202 (2004).
The nominal test item concentrations of 1.1, 2.3, 5.0, 11, 23 and 50 mg/L were tested. Additionally, a control group was tested in parallel. Since the test item was not stable over the test medium renewal periods (24 hours), the reported biological results are based on the mean measured test item concentrations (calculated as the arithmetic mean of the two geometric means of the concentrations measured at the start and end of the test medium renewal periods). The measured concentrations were 0.36, 0.75, 1.9, 3.8, 8.0 and 17 mg/L. The biological test results were as follows:
48-hour EC50: 3.8 mg/L
48-hour NOEC: 0.75 mg/L
48-hour EC100: 17 mg/L
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