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EC number: 930-389-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- June 29, 2007 - August 17, 2007
- Reliability:
- 1 (reliable without restriction)
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- , 2004
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- signed 2006-06-01
Test material
Reference
- Name:
- Unnamed
- Type:
- Constituent
- Type:
- Constituent
- Details on test material:
- - Name of test material (as cited in study report): Symrose
- Substance type: technical product
- Physical state: colourless, liquid
- Storage condition of test material: ambient temperature, dark and dry
No further details are given.
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
No further test material was used.
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- The amount of test material exposed to the organisms was analysed via GC after 0 and 24 hours (new media) and 24 and 48 hours (old media).
Preparation of standards for validation of the analytical method:
- Sampling method: Five replicates of test medium fortified with 100 μg/L of the standard and two dilution water blank samples were analysed. Two dilution water blank samples were used to prove specificity and blank values being < 30 % of the LOQ.
- Sample storage conditions before analysis: All samples were stored at 7 ± 2 °C until start of analysis, if necessary.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The stock solution (100mg/L test item were weighed out) was prepared with dilution water one day prior tp application. The stock solution was stirred in a closed bottle with 110rpm for 24 hours at ambient temperature.
- Controls: Dilution water without test item tested under the same conditions as the test groups.
- Evidence of undissolved material: Undissolved particles were removed by centrifugation for 10 minutes at 3000rpm and membrane filtration (0.2µm).
No further details are given.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna (20 animals, divided into 4 parallel samples, each with 5 animals)
- Strain: STRAUS (Clone 5)
- Source: Institute für Wasser-, Boden- und Lufthygiene (WaBoLu)
- Age at study initiation: 2-24 hours
- Method of breeding: In 2-3L glass vessels with approximately 1.8L culture medium, at 20 +/- 2°C, in an incubator, 16 hours illumination
- Feeding during test: The daphnids were not fed during the test.
- Acclimation period: at least 2 hours in dilution water
No further details are given.
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- none
Test conditions
- Hardness:
- total hardness of dilution water: 246 - 253 mg CaCO3/L
- Test temperature:
- 18-20°C +/- 1°C constant
- pH:
- 7.8 +/- 0.2
- Dissolved oxygen:
- new media:
- 0 hours: 6.68 to 8.26 mg O2/L
- 24 hours: 5.91 to 8.21 mg O2/L
old media:
- 24 hours: 7.43 to 7.83 mg O2/L
- 48 hours: 7.32 to 7.87 mg O2/L
dilution water: 8.15 to 8.21 mg O2/L - Salinity:
- According to Directive 92/69/EC L383A C.2. Annex
KCl: 5.76 mg/L
NaHCO3: 64.8 mg/L
CaCl2 x 2H2O: 294 mg/L
MgSO4 x 7H2O: 123 mg/L - Nominal and measured concentrations:
- Nominal test item concentrations: 0.88, 1.94, 4.27, 9.39, 20.7, 45.5 and 100 mg/L (factor 2.2)
Geomeric mean measured concentration of the active ingredient: 0.60, 1.39, 3.04, 6.98, 14.9, 32.5 and 81.5 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers (4cm ID x 7cm H), 50mL
TEST DESCRIBTION:
A preliminary range finding test was conducted with three test item concentrations ranging from 1 to 100 mg/L set up in a geometric series with a dilution factor of 10 under semi-static conditions. Based on the results the definitive test was performed with 7 concentrations ranging from nominal 0.88 to 100 mg/L set up in a geometric series with a dilution factor of 2.2, corresponding to mean measured concentrations of the active ingredient of 0.604 to 81.9 mg/L, under semi-static conditions to enable the determination of immobilisation after 24 and 48 hours.
Water parameters were measured at 0 and 24 hours (new media) and 24 and 48 hours (old media).
TYPE AND FREQUENCY OF MEASURMENT AND OBSERVATIONS:
Prior to test start (0 hours) and after 24 hours (water renewal) pH values, dissolved oxygen concentration, temperature, conductivity and total hardness of the dilution water were measured.
In new media the water parameters were measured in one additional replicate per concentration and control. In the old media the water parameters were measured in all replicates per concentration and control. The room temperature was recorded throughout the test with a thermohygrograph. The percentage immobility was determined in all test and control groups after 24 and 48 hours. An animal was considered to be immobile, if it was not able to swim 15 second after gentle agitation of the test vessel. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromat
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 3.04 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.39 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 14.9 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC100
- Effect conc.:
- 32.51 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 7.81 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 7.33 - 8.33 mg/L confidence interval p= 95%
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 10.7 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 9.58 - 11.8 mg/l confidence interval p= 95%
- Duration:
- 48 h
- Dose descriptor:
- EC10
- Effect conc.:
- 5.07 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC10
- Effect conc.:
- 6.95 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- no relevant observations
- Results with reference substance (positive control):
- Reference item: potassium dichromate p.a. (SIGMA)
Test concentration: 1.66 – 1.99 – 2.39 – 2.87 mg/L
- Results with reference substance valid? yes
- EC50: 2.04 (CI 1.88 - 2.21) after 24h
The EC50-value of the reference item potassium dichromate after 24 h is within the prescribed concentration range 0.6 mg/L to 2.1 mg/L, according to OECD 202 (2004). - Reported statistics and error estimates:
- Statistical methods: EC100 -values after 24 and 48 hours were deducted directly from the dose-response-relationship. There was no mathematical calculation. The 25h and 48h EC10 and EC50-values were calculated by sigmoidal dose-response regression.
Any other information on results incl. tables
The validation of the analytical method was performed in accordance with SANCO/3029/99 rev. 4 (11/07/00):
Linearity: The analytical system gave linear response in the calibration range of 10 to 800 μg/L. The correlation coefficient (r) of the calibration curves was ≥ 0.99.
LOQ: The LOQ of the analytical method was fixed at nominal 100 μg/L of the test item and checked by means of accuracy
The mean recovery rate (fortification level 100 µg/L n=5) was 75% with a RSD of 2.89. The response of blank values of test medium control samples was lower than 30 % of LOQ
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- (i) In the control group no Daphnia were immobilised or trapped on the surface of the water (ii) The EC50 -value after 24 h of the reference item was in the range of 0.6 to 2.1 mg/L (iii) The dissolved O2 concentration was > 3 mg/L (iv) The pH-value did n
- Conclusions:
- Symrose was found to be toxic to Daphnia magna after 48 hours. The EC50 (48 hours) was 7.86 (7.37 – 8.38) mg/L. The NOEC after 48 hours was 1.40 mg/L. The LOEC after 48 hours was 3.06 mg/L.
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