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EC number: 947-993-5 | CAS number: -
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Not yet reported
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- >= 3.2 - <= 5.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- loading rate WAF
- Basis for effect:
- mobility
- Details on results:
- - No biologically relevant immobility (i.e. >10%) was observed in the control and at the three lowest test concentrations throughout the test.
- At the end of the test, 85 and 100% immobility was observed at the WAFs prepared at loading rates of 5.6 and 10 mg/L, respectively.
- It should be noted that 10 % immobility is allowed in the control and therefore, effects ≤ 10 % are considered to be biologically not relevant. - Validity criteria fulfilled:
- yes
- Conclusions:
- The 48-hour EC50 value is expressed in terms of loading rate and is considered to lie between 3.2 and 5.6 mg/L loading rate WAF.
- Executive summary:
GUIDELINE
A study was performed to assess the acute toxicity of the test item toDaphnia magna.The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (April 2004) No 202,"Daphniasp., Acute Immobilisation Test" referenced as Method C.2 of Commission Regulation (EC) No. 440/2008.
METHODS
For the final test, Water Accommodated Fractions (WAFs) were individually prepared at loading rates of 1.0, 1.8, 3.2, 5.6 and 10 mg/L. One hour of gentle magnetic stirring was applied to accelerate dissolution of test item in test medium. The obtained mixtures were allowed to settle for a period of approximately two hours. Thereafter, the WAFs were collected by means of siphoning over glass wool and used as test concentrations. At the end of the preparation procedure, all test solutions were clear and colourless.
RESULTS
No biologically relevant immobility (i.e. >10%) was observed in the control and at the three lowest test concentrations throughout the test. At the end of the test, 85 and 100% immobility was observed at the WAFs prepared at loading rates of 5.6 and 10 mg/L, respectively. It should be noted that 10 % immobility is allowed in the control and therefore, effects ≤ 10 % are considered to be biologically not relevant.
CONCLUSION
The 48-hour EC50 value is expressed in terms of loading rate and is considered to lie between 3.2 and 5.6 mg/L loading rate WAF.
Reference
NUMBER OF INTRODUCED DAPHNIDS AND INCIDENCE OF IMMOBILITY IN THE FINAL TEST
Time (hours) |
Replicate |
Control (mg/L) |
Test item 1.0 mg/L loading rate WAF |
Test item 1.8 mg/L loading rate WAF |
Test item 3.2 mg/L loading rate WAF |
Test item 5.6 mg/L loading rate WAF |
Test item 10 mg/L loading rate WAF |
0 |
A |
5 |
5 |
5 |
5 |
5 |
5 |
0 |
B |
5 |
5 |
5 |
5 |
5 |
5 |
0 |
C |
5 |
5 |
5 |
5 |
5 |
5 |
0 |
D |
5 |
5 |
5 |
5 |
5 |
5 |
0 |
Total |
20 |
20 |
20 |
20 |
20 |
20 |
24 |
A |
0 |
0 |
0 |
0 |
2 |
4 |
24 |
B |
0 |
0 |
0 |
0 |
3* |
3 |
24 |
C |
0 |
0 |
1 |
0 |
2 |
2 |
24 |
D |
0 |
0 |
0 |
0 |
3 |
3 |
24 |
Total immobilised |
0 |
0 |
1 |
0 |
10 |
12 |
24 |
Effect % |
0 |
0 |
5 |
0 |
50 |
60 |
48 |
A |
0 |
0 |
0 |
0 |
4 |
5 |
48 |
B |
0 |
0 |
1 |
0 |
5 |
5 |
48 |
C |
0 |
0 |
1 |
0 |
3 |
5 |
48 |
D |
0 |
0 |
0 |
0 |
5 |
5 |
48 |
Total immobilised |
0 |
0 |
2 |
0 |
17 |
20 |
48 |
Effect % |
0 |
0 |
10 |
0 |
85 |
100 |
*Microscopic observation revealed that no test item was attached to the daphnids.
ANALYTICAL RESULTS
Sampling time (hours) |
Date of sampling |
Date of analysis |
Loading rate (mg/L) |
Concentration analysed (mg/L) |
Relative to initial (%) |
Note |
0 |
14 May 2018 |
23 May 2018 |
0 |
0.00065 |
Not applicable |
1 |
0 |
14 May 2018 |
23 May 2018 |
1.0 |
0.014 |
Not applicable |
1 |
0 |
14 May 2018 |
23 May 2018 |
1.8 |
0.112 |
Not applicable |
- |
0 |
14 May 2018 |
23 May 2018 |
3.2 |
0.0969 |
Not applicable |
- |
0 |
14 May 2018 |
23 May 2018 |
5.6 |
0.209 |
Not applicable |
- |
0 |
14 May 2018 |
23 May 2018 |
10 |
0.266 |
Not applicable |
- |
48 |
16 May 2018 |
23 May 2018 |
0 |
< 0.000091 |
< 14 |
2 |
48 |
16 May 2018 |
23 May 2018 |
1.0 |
< 0.000091 |
< 0.65 |
2 |
48 |
16 May 2018 |
23 May 2018 |
1.8 |
0.00086 |
0.76 |
1 |
48 |
16 May 2018 |
23 May 2018 |
3.2 |
0.00063 |
0.65 |
1 |
48 |
16 May 2018 |
23 May 2018 |
5.6 |
0.0015 |
0.71 |
1 |
48 |
16 May 2018 |
23 May 2018 |
10 |
0.0021 |
0.78 |
1 |
1 = Estimated value, calculated by extrapolation of the calibration curve.
2 = Not detected. The limit of detection of the method was determined to be 0.0912 µg/L taking a dilution factor of 2 into account.
Description of key information
The 48-hour EC50 value is expressed in terms of loading rate and is considered to lie between 3.2 and 5.6 mg/L loading rate WAF.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 3.2 mg/L
Additional information
GUIDELINE
A study was performed to assess the acute toxicity of the test item toDaphnia magna.The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (April 2004) No 202,"Daphniasp., Acute Immobilisation Test" referenced as Method C.2 of Commission Regulation (EC) No. 440/2008.
METHODS
For the final test, Water Accommodated Fractions (WAFs) were individually prepared at loading rates of 1.0, 1.8, 3.2, 5.6 and 10 mg/L. One hour of gentle magnetic stirring was applied to accelerate dissolution of test item in test medium. The obtained mixtures were allowed to settle for a period of approximately two hours. Thereafter, the WAFs were collected by means of siphoning over glass wool and used as test concentrations. At the end of the preparation procedure, all test solutions were clear and colourless.
RESULTS
No biologically relevant immobility (i.e. >10%) was observed in the control and at the three lowest test concentrations throughout the test. At the end of the test, 85 and 100% immobility was observed at the WAFs prepared at loading rates of 5.6 and 10 mg/L, respectively. It should be noted that 10 % immobility is allowed in the control and therefore, effects ≤ 10 % are considered to be biologically not relevant.
CONCLUSION
The 48-hour EC50 value is expressed in terms of loading rate and is considered to lie between 3.2 and 5.6 mg/L loading rate WAF.
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