Registration Dossier
Registration Dossier
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EC number: - | CAS number: -
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
ErC50 (72h) = 38.3 mg/l, based on nominal concentration
Key value for chemical safety assessment
Additional information
The toxicity of the test itemto the unicellular freshwater green alga Pseudokirchneriella subcapitata was determined according to the principles of OECD 201 andCouncil Regulation (EC) No. 266/2016/Method C.3.The aim of the study was the determination of the effects on growth rate and yield over a period of 72 hours.
The study was conducted under static conditions with an initial cell density of 5416 cells/mL. A stock solution with a nominal concentration of 100 mg test item/L was freshly prepared with dilution water. This dispersion was stirred for 30 minutes (room temperature, 1100 rpm) with a magnetic stirrer. The stock solution was checked via laser beam (Tyndall effect) for undissolved test item. The Tyndall effect was negative. Five concentrations were tested in a geometrical series with a spacing factor of √10: 1.00 - 3.16 - 10.0 - 31.6 - 100 mg/L.
Three replicates were tested for each test item concentration and six replicates for the control. The environmental conditionswere within the acceptable limits.
The concentrationsthe test itemwereanalytically verified via spectrophotometric analysis at the start (0 hours) and at the end of the exposure (72 hours).
The measured concentrations of the test item at the start of the exposure(0 hours) were in the range of < LOQ to 152% of the nominal values, after 24 hours < LOQ to 193%, after 48 hours < LOQ to 187% and at test end (72 hours) in the range of < LOQ to 181% of the nominal values.
All effect values given are based on the nominal test item and are summarized as follows:
NOEC, LOEC, ECx-values(0 - 72 hours)(based on the nominal test item concentrations, with 95% confidence intervals)
|
Growth Rate Inhibition |
NOEC |
1.00 |
LOEC |
3.16 |
ErC10 |
11.7 (8.98 – 15.3) |
ErC20 |
19.5 (16.5 – 22.0) |
ErC50 |
38.3 (35.8 – 42.3) |
|
Yield Inhibition nominal test item concentrations, with 95% confidence intervals [mg/L] |
NOEC |
< 1.00 |
LOEC |
1.00 |
EyC10 |
n.a. |
EyC20 |
3.40 (< 1.00 – 7.91) |
EyC50 |
13.6 (10.3 – 18.2) |
n.a. = not applicable
The
EC50-values for inhibition of growth rate (ErC50)
and yield (EyC50) after 72 hours were 38.3 (35.8 –
42.3) mg/L and 13.6 (10.3 – 18.2) mg/L, respectively. The NOEC for
inhibition of growth rate and yield was 1.00 and < 1.00 mg/L,
respectively.
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