Cuprate(4-), [μ-[[7,7'-[[6-[(2-hydroxyethyl)amino]-1,3,5-triazine-2,4-diyl]diimino]bis[4-(hydroxy-κO)-3-[[2-(hydroxy-κO)-5-sulfophenyl]azo-κN1]-2-naphthalenesulfonato]](8-)]]di-, tetrasodium

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From September 21, 2016 to November 30, 2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))

GLP compliance:

yes

Test organisms (species):

activated sludge

Details on inoculum:

- Source of inoculum/activated sludge: Dihua sewage treatment plant, Taiwan.

Test temperature:

20 ± 2 °C

pH:

6.83-7.75

Nominal and measured concentrations:

10, 100 and 1000 mg/L

Details on test conditions:

- Aeration: The test mixture was incubated under conditions of forced aeration at 0.5-1.0 L/min with air removed dust and oil.
- No. of vessels per abiotic control (replicates): three
- Sludge concentration: 1.50 g/L
- Nitrification inhibitor used : N-allylthiourea

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorophenol

Key result

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Table 1. Oxygen consumption rates for the preliminary test

Group (mg/L)	Oxygen consumption rate (mg/Lh)	Average	Coefficient of variation (%)
Blank control group	33.84	34.98	4.61
	36.12
Nitrification control group	35.76	─	─
Test group
10	31.56	─	─
100	41.04	─	─
1000	28.68	30.16	11.74
1000	27.60
1000	34.20
Reference control group
1	31.92	─	─
10	25.44	─	─
100	7.68	─	─
Abiotic control group	9.72	9.88	20.70
	12.00
	7.92

Table 2. Inhibition rates of total oxygen uptake and EC₅₀ for the preliminary test

Group (mg/L)	Inhibition rate of total oxygen uptake (%)	Average	EC50(mg/L)
Test group
10	9.78	─	>1000.00
100	-17.32	─
1000	18.01	13.78
1000	21.10
1000	2.23
Reference control group
1	8.75	─	22.17
10	27.27	─
100	78.04	─

Validity criteria fulfilled:

yes

Conclusions:

According to OECD 209 test method, the EC50 of Everdirect SH12 for oxygen consumption rate of sludge was greater than 1000 mg/L.

Executive summary:

This test using the procedures outlined in the SuperLab Study Plan for M62-151100088001EN which is based on the SOP for the OECD 209 (SOPE-508) and OECD 209 (OECD, 2010). The results of this OECD 209 test for Everdirect SH12 show that all validity criteria were met. The EC₅₀ of Everdirect SH12 for oxygen consumption rate of sludge was greater than 1000 mg/L.

 

Description of key information

The EC₅₀ of Everdirect SH12 for oxygen consumption rate of sludge was greater than 1000 mg/L (OECD TG 209).

Key value for chemical safety assessment

EC50 for microorganisms:

1 000 mg/L

Additional information

For blank control groups at the end of the test, the coefficient of variation of oxygen consumption rate were less than 30% and greater than 20.00 mg/h of per one gram of sludge, respectively. The EC50 of reference control group (3,5-Dichlorophenol) was lied in the range between 2.00 and 25.00 mg/L. All test validity criteria were met. The EC50 of Everdirect SH12 for oxygen consumption rate of sludge was greater than 1000 mg/L.