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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From Auguest 03, 2016 to December 12, 2016
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes
Test organisms (species):
Daphnia magna
Details on test organisms:
- Source: National Chiayi University Freshwater Biological Resource Center
Water media type:
freshwater
Total exposure duration:
48 h
Test temperature:
18-22 °C
Nominal and measured concentrations:
For the range-finding test: 0.16, 0.80, 4.00, 20.00 and 100.00 mg/L
For the definitive test: 0.16, 0.35, 0.77, 1.70, 3.75, 8.25, 18.14 and 39.91 mg/L
Details on test conditions:
- Test volume: 30 mL
- Aeration: None
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Photoperiod: 16-hr light: 8-hr dark
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
3.34 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

In range-finding test, the 0% of control organisms were immobilised at the end of the test and the dissolved oxygen concentration of control and test groups during the test was 8.25-8.67 mg O2/L. The initial pH measurements did not differ from the final measurements by more than 1.5 units in each test. The results of the range-finding test showed that EC50was 8.46 mg/L.

In limit test, the results showed below:

Table 1. The numbers immobilized forthe definitive teston day 1 and 2

Days

Group

(mg/L)

Numbers of no immobilised

Numbers of activity immobilised

Numbers of discoloration

Numbers of trapping at surface of water

Day1

Control group

0.00

20

0

0

0

Test group

39.91

0

19

0

1

18.14

0

20

0

0

8.25

0

18

0

2

3.75

7

11

0

2

1.70

14

6

0

0

0.77

18

2

0

0

0.35

20

0

0

0

0.16

20

0

0

0

Day2

Control group

0.00

20

0

0

0

Test group

39.91

0

20

0

0

18.14

0

20

0

0

8.25

0

20

0

0

3.75

5

15

0

0

1.70

14

6

0

0

0.77

16

4

0

0

0.35

19

1

0

0

0.16

18

2

0

0

Table 2. The percentages immobilized for the definitive test

Group

(mg/L)

Percentages immobilized (%)

Day 0

Day 1

Day 2

Control group

0.00

0.00

0.00

0.00

Test group

39.91

0.00

100.00

100.00

18.14

0.00

100.00

100.00

8.25

0.00

100.00

100.00

3.75

0.00

65.00

75.00

1.70

0.00

30.00

30.00

0.77

0.00

10.00

20.00

0.35

0.00

0.00

5.00

0.16

0.00

0.00

10.00

Table 3. EC50 and 95% confidence interval forthe definitive test

Parameter

Concentration

(mg/L)

EC50

3.34

95% confidence interval-lower

2.10

95% confidence interval-upper

5.03

Table 4. Temperature, dissolved oxygen and pH value for the definitive test

Group

(mg/L)

Day 0

Day 2

Temperature

(°C)

Dissolved

oxygen

(mg/L)

pH

value

Temperature

(°C)

Dissolved

oxygen

(mg/L)

pH

value

Control group

0.00

20.0

7.69 ± 0.03

7.21 ± 0.12

20.0

8.23 ± 0.15

7.00 ± 0.07

Test group

39.91

7.88 ± 0.03

7.42 ± 0.02

8.59 ± 0.02

7.53 ± 0.05

Validity criteria fulfilled:
yes
Conclusions:
According to OECD 202 test method, the 48-hr EC50 of Everdirect SH12 was 3.34 mg/L.
Executive summary:

This test using the procedures outlined in the SuperLab Study Plan for M62-151100087001EN which is based on the SOP for the OECD 202 (SOPE-505) and OECD 202 (OECD, 2004). The results of this OECD 202 test for Everdirect SH12 show that all validity criteria were met. Under the conditions of this test, the 48-hr EC50 of Everdirect SH12 was 3.34 mg/L.

 

Description of key information

The 48-hr EC50 of Everdirect SH12 was 3.34 mg/L for Daphnia magna (OECD TG202).

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
3.34 mg/L

Additional information

In both range-finding and definitive test, not more than 10 % of the Daphnia magna were immobilised in the control and the dissolved oxygen concentration of control and test groups at the end of the test was ≥3 mg O2/L. All test validity criteria were met. The pH of the test solutions was within the ranges outlined in the OECD 202 test guideline. Under the conditions of this test, the 48-hr EC50 of Everdirect SH12 was 3.34 mg/L.