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Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
13 - 17 Jun 2016
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2016
Report date:
2016

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Qualifier:
according to guideline
Guideline:
EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
GLP compliance:
yes (incl. QA statement)

Test material

1
Reference substance name:
Sucrose tetrastearate triacetate
IUPAC Name:
Sucrose tetrastearate triacetate

Sampling and analysis

Analytical monitoring:
no
Remarks:
Due to the complexity of the product, there is no suitable method for measuring the test solution concentrations.

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was weighed in a tared glass beaker for each concentration. The substance was then rinsed with dilution water into glass aspirator bottles to a final volume of 3 L. The test solutions were mixed for about 48 hrs. After the mixing, the solutions were allowed to settle for 4 hrs, and then decanted into test chambers from spigot and tubing placed 2-3 cm from the bottom.
- Controls: A negative control was prepared using only dilution water. The negative control solution was clear throughout the test with no evidence of precipitate.
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): Yes, all test solutions had a white color and precipitate when first made. By the end of the test, the 63-500 mg/L test solutions were colorless, but showed white precipitate at the bottom of the test vessels. The 1000 mg/L solution was still slightly white in color with white precipitate at the bottom and in the water column.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Source: Wildlife International of Easton, Maryland, USA
- Age of parental stock (mean and range, SD): At least 28 days, and had produced at least one previous brood.
- Feeding during test: no

METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES: Neonates < 24 hrs old were used in the test.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Hardness:
144-148 mg/L CaCO3
Test temperature:
19.8 - 20.6 °C
pH:
8.1 - 8.4
Dissolved oxygen:
≥ 91%
Conductivity:
329-359 µS/cm
Nominal and measured concentrations:
Nominal: 0, 63, 125, 500, 1000 mg/L
Measured: Concentrations were not measured due to the lack of a suitable analytical method.
Details on test conditions:
TEST SYSTEM
- Test vessel: 250 mL glass beakers
- Material, size, headspace, fill volume: glass, 250 mL, filled with 220 mL test solution
- Aeration: No
- Type of flow-through (e.g. peristaltic or proportional diluter): static test
- Renewal rate of test solution (frequency/flow rate): not renewed
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: well water, 40 m deep
- Total organic carbon: < 1 mg C/L
- Metals: Below limit of detection.
- Pesticides: Below limit of detection.
- Chlorine: 4.3 mg/L
- Alkalinity: 176-178 mg/L CaCO3
- Ca/mg ratio: 34.9/13.4 mg
- Conductivity: 329-359 uS/cm
- Culture medium different from test medium: No
- Intervals of water quality measurement: Within 4-weeks of test.

OTHER TEST CONDITIONS
- Photoperiod: 16 hrs light/8 hrs dark with 30 min transition period.
- Light intensity: 591 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Observations for immobility, abnormal behavior, and signs of toxicity were made at 3, 24, and 48 hrs after start of exposure.
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EL50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOELR
Effect conc.:
>= 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
- Behavioural abnormalities: One daphnid in the 125 mg/L group was lethargic at the 24 hr observation. As this daphnid recovered, and no other daphnids at this or the 500 mg/L concentration showed lethargy, this was considered incidental. Two daphnids in the 1000 mg/L concentration group were lethargic at the 24 hr observation, but not at the 48 hr observation.
- Mortality of control: None
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: White color of the test solutions and precipitate was noted at all test concentrations.
- Effect concentrations exceeding solubility of substance in test medium: yes
Reported statistics and error estimates:
Since no effects were visible in this study statistical analysis were not necessary.

Any other information on results incl. tables

 Concentration

Number Immobile

3 hr Observation

 

Number Immobile

 24 hr Observation
 

Number Immobile

48 hr Observation
 Control  0  0  0
 63 mg/L  0  0
 125 mg/L  0  0  0
 500 mg/L  0  0  0
 1000 mg/L  0  0  0

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 48-hr EL50 for daphnids is > 1000 mg/L (WAF).
Executive summary:

The acute toxicity of the test substance to Daphnia magna was determined in an OECD Guideline 202 study. Daphnids were exposed to loading rates of 0, 63, 125, 500, 1000 mg/L (water accommodated fraction) of test substance. At the 24 hr observation, some daphnids showed lethargy, but all had recovered by the 48 hr observation. The EL50 (48 h) for the test substance is >1000 mg/L (WAF).