Registration Dossier
Registration Dossier
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EC number: 941-453-2 | CAS number: -
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
Based on the results obtained in studies conducted according to OECD Guideline 203 (Biofert Plusz) and national guideline (L-threonine mother liquor and modified waste), respectively, the 96 h LC50=738 mg dry matter/L (measured concentration) found for Biofert Plusz with Danio rerio as test organism can be regarded as worst case for the toxicity of L-threonine mother liquor.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 738 mg/L
Additional information
The acute toxicities of L-threonine mother liquor and modified waste (used microbiological material) to Poecilia reticulata were investigated in studies conducted according to a national standard method. In respect to L-threonine mother liquor and modified waste a 48 h LC50=105 mL/L and a 96 h LC50=39.57 mL/L (nominal each) were determined, respectively. In a further study conducted according to OECD Guideline 203, a 96 h LC50>100 mg/L (nominal) was found for the same fish species using modified waste.
The acute toxicity of Biofert Plusz (mixture of L-threonine mother liquor and L-threonine biomass; L-threonine biomass is also named as modified waste (used microbial material sterilized), mortified biomass, mortified biomass of E. coli (K12), biomass concentrate of threonine production stem) towards Danio rerio was investigated in a study conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test) under semi-static conditions. Fish were exposed for 96 h to measured Biofert Plusz concentrations ranging from 222 to 3861 mg fresh weight/L, equivalent to 62.3 -1085 mg dry matter/L. Analytical monitoring of test material concentration was performed by HPLC and quantification of the main component (L-threonine). The 96 h LC50 was determined to be 738 mg dry matter/L (effective).
In conclusion of the available data, it can be assumed that the result obtained with Biofert Plusz represents a worst case situation for the test material under investigation (L-threonine mother liquor).
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