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EC number: 404-910-2 | CAS number: 164578-14-7 PIGMENT ADDITIV C
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Oct 1989 - Feb 1990
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 4. April 1984
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2-(4-(diethylaminopropylcarbamoyl)phenylazo)-3-oxo-N-(2,3-dihydro-2-oxobenzimidazol-5-yl)butyramide
- EC Number:
- 404-910-2
- EC Name:
- 2-(4-(diethylaminopropylcarbamoyl)phenylazo)-3-oxo-N-(2,3-dihydro-2-oxobenzimidazol-5-yl)butyramide
- Cas Number:
- 164578-14-7
- Molecular formula:
- C25H31N7O4
- IUPAC Name:
- N-[3-(diethylamino)propyl]-4-[(1E)-2-{2-oxo-1-[(2-oxo-2,3-dihydro-1H-1,3-benzodiazol-5-yl)carbamoyl]propyl}diazen-1-yl]benzamide
- Test material form:
- solid: bulk
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations:
Trial I: 10, 18, 32, 56, 100, 180 mg/L
Trial II: 1, 1.8, 3.2, 5.6 mg/L
- Sampling method:
At the start of the exposure (0 hours) and at the end of the exposure (48 hours), samples for analyses of the freshly prepared and old media were taken from all test replicates.
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:
For preparation of the 1 - 5.6 mg/L concentrations 0.02 mL Tween 80 were added to the test item and filled up with water. After sonification for 30 min and stirring for 5 min the solution was filled up to 200 mL end volume with water
.The 10 to 180 mg/L test concentrations were directly weighed in the test vessels and moistened with 0.095 mL Tween 80/L. Afterwards 200 mL water was added and the suspensions were homogenised by sonication and ultra-turrax.
Water: dilution water according to ISO/DIS 7346/1
- Controls:
solvent control: 0.095 mL Tween 80/L
- Chemical name of vehicle (organic solvent, emulsifier or dispersant):
Tween 80
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)):
0.095 mL/L
- Evidence of undissolved material (e.g. precipitate, surface film, etc.):
1-18 mg/L samples: clolorless to yellow solution
18 mg/L: slight precipitation
32-180 mg/L samples: precipation at the begin of experiment, at termination yellow solution
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: STRAUS
- Justification for species other than prescribed by test guideline:
Dahpnia magna is the preferred species in accordance with the test guideline and is bred at the test facility.
- Source: Hoechst AG
- Age of parental stock (mean and range, SD): 3 weeks
- Feeding during test: no feeding during exposure time
- Food type:
for breeding: green algae (scendesmus subspicatus) and a suspension of Tetra min dry food (Tetra Werke, Melle, Germany)
- Culture: in glass vessels (3 L capacity) with water at 20+/-1°C, 12 h illumination
ACCLIMATION
- Acclimation period: 6 h
Study design
- Test type:
- static
- Water media type:
- other: water according to ISO/DIS 7346/1
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- none
Test conditions
- Hardness:
- no data
- Test temperature:
- test groups: 20.6 - 21.8°C
control: 20.4 - 21.1°C - pH:
- test groups: 8.1 - 8.7
control: 8.1 - 8.3 - Dissolved oxygen:
- test groups: 8.2 - 9.2 mg/L
control: 8.4 - 9.8 mg/L - Salinity:
- no data
- Conductivity:
- no data
- Nominal and measured concentrations:
- Nominal concentrations:
1, 1.8, 3.2, 6.5, 10, 18, 32, 56, 100 and 180 mg/L
Measured concentrations:
0.9, 1.6, 2.6, 5.2, 9.4, 19.9, 33.7, 58, 101.5 and 182.5 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 300 mL cristallisation dishes (55 mm heigth, 95 mm diameter)
- Material, size, headspace, fill volume: 32 mm
- Aeration: none
- No. of organisms per vessel: 10
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
immobilisation
VEHICLE CONTROL PERFORMED: yes
RANGE-FINDING STUDY PERFORMED: yes
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC0
- Effect conc.:
- 5.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 3.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 41.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 7.72 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 18 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
Any other information on results incl. tables
At test item concentration above 10 mg/L the solutions were colored yellow. The measured water quality parameters (i.e. pH, oxygen concentration and temperature) as well as the analysed test item concentrations were within acceptable ranges.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the nominal (and measured) concentrations of the test item, the 48-hour EC50 for Daphnia magna was 7.72 mg/L.
- Executive summary:
In the acute immobilization test with Daphnia magna (STRAUS), the effects of the test item were determined at the test facility according to OECD 202 (1994).
The study was conducted under static conditions over a period of 48 hours with a test item solution with the nominal concentrations of 1, 1.8, 3.2, 5.6, 10, 18, 32, 56, 100 and 180 mg/L of the test item.
At test item concentration above 10 mg/L the solutions were colored yellow. Ten daphnids were exposed each concentration level and the control.
The concentrations of the test item were analytically verified via photometry in fresh media at the start (0 hours) and in old media at the end of the exposure (48 hours) in each concentration and in the control.
The measured concentrations of the test item were in the range of 89 to 111% of the nominal value.
The measured test item concentrations remained stable within ± 20% of the nominal limit concentration of the test item over the test period.
The validity criteria of the test guideline were fulfilled.
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