Fenugreek, ext.

Boiling point

Link to relevant study record(s)

Reference

Endpoint:

boiling point

Type of information:

experimental study

Adequacy of study:

key study

Study period:

09 January - 28 March 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

The study was conducted according to an internationally recognised method, and under GLP. The test substance is adequately characterised. Therefore full validation applies.

Qualifier:

according to guideline

Guideline:

OECD Guideline 103 (Boiling Point)

Version / remarks:

1995

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.2 (Boiling Temperature)

Version / remarks:

2008

Deviations:

no

Principles of method if other than guideline:

Not applicable

GLP compliance:

yes (incl. QA statement)

Remarks:

inspected on 20 and 21 June 2017 / signed on 04 September 2017

Type of method:

differential scanning calorimetry

Key result

Boiling pt.:

ca. 110 °C

Atm. press.:

ca. 101.325 kPa

Decomposition:

ambiguous

Results

-DSC measurement

Two DSC measurements in aluminium crucibles with a hole showed a multistage endothermal effect above ambient temperature starting at 115 °C (see Table 4.3/1). The mass loss after the DSC measurements amounted to 6 ‑ 90 %.

Table 4.3/1.- Boiling point (DSC measurement)

Ident No.	Test item / mg	Starting temperature / °C	Final test temperature / °C	Temperature range (endo-thermal) / °C	Onset  (boiling) / °C	Crucible	Observations after the measurement
36809	17.91	-50	500	120 – 160	multi-stage	Aluminium with a hole	Mass loss 90 %
36831	16.82	25	200	115 – n.e.	multi-stage	Aluminium with a hole	Mass loss 6 %

n.e. not evaluable

Graphical presentations of the DSC runs are shown in the experimental report

In order to clarify the boiling point an additional measurement with the capillary method was performed.

-Capillary method

A measurement with the capillary method was performed to clarify the results of the DSC measurements. Since this measurement was performed only as a verifying screening a high heating rate of 10 K/min was chosen and therefore the absolute temperature values derived with the capillary method are not as accurate as the DSC results. The filling height of the test item was approximately 5 mm.

Table 4.3/2.- Results of the capillary method

No.	Set point / °C	Heating rate / K/min	End point / °C	Remarks
1	100	10	250	Approx. 100 °C: minor formation of bubbles Approx. 110 °C: increasing formation of bubbles Approx. 188 °C: starting discolouration and ongoing formation of bubbles Approx. 218 °C: increasing formation of bubbles Approx. 250 °C: discoloured to black and ongoing formation of bubbles

Under consideration of the results from the DSC measurements and the capillary method it was concluded that the test item boiled under potential decomposition starting at approximately 110 °C. The DSC measurements indicated a multistage boiling.

The test item started to boil under potential decomposition at approximately 110 °C

Conclusions:

The test item started to boil under potential decomposition at approximately 110 °C.

Executive summary:

The study was performed in order to determine the boiling point of test item according to OECD 103, resp. EU A.2, guideline, using the DSC method.

Two DSC measurements in aluminium crucibles with a hole showed a multistage endothermal effect above ambient temperature starting at 115 °C. The mass loss after the DSC measurements amounted to 6 ‑ 90 %. In order to clarify the boiling point an additional measurement with the capillary method was performed.

At about 110 °C increasing formation of bubbles was observed.

Under consideration of the results from the DSC measurements and the capillary method it was concluded that the test item boiled under potential decomposition starting at approximately 110 °C.

Description of key information

The test item started to boil under potential decomposition at approximately 110 °C.

Key value for chemical safety assessment

Boiling point at 101 325 Pa:

110 °C

Additional information

A fully reliable experimental study, conducted according to OECD 103/ EU A2 guideline is available.

It is considered as a key study and the result is retained as key data.