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EC number: 283-415-1 | CAS number: 84625-40-1 Extractives and their physically modified derivatives such as tinctures, concretes, absolutes, essential oils, oleoresins, terpenes, terpene-free fractions, distillates, residues, etc., obtained from Trigonella foenum-graecum, Leguminosae.
Table 7.3.2/1: Main test - Individual and mean corrected OD values and tissue viabilities for the test item, the negative and positive controls
Mean OD/disc (#)
Mean viability %
Difference of viability %
#: mean of 3 values (triplicate of the same extract)
OD: optical density
Test item was applied, as supplied, at the dose of 50 µL, to 2 living DPBS pre-treated RhCE (EpiOcularTMtissue model) during 30 minutes at 37°C, 5% CO2, 95% humidity (standard culture conditions). The exposure period was followed by extensive rinsing with DPBS at room temperature, a 12 minutes post-exposure immersion period at room temperature and a 2 hours post-exposure incubation at standard culture conditions. The tissue viability was measured by performing an MTT assay.
The experimental protocol was established in accordance with O.E.C.D. Test Guideline No. 492 adopted 09 October 2017.
The mean percent tissue viability of the RhCE replicates treated with test item was 96.49% versus 17.72% in the positive control (Methyl acetate).
In conclusion, under the experimental conditions adopted and in accordance with Regulation EC No. 1272/2008, test item does not require classification for eye irritation or serious eye damage. It corresponds to the UN GHS No Category.
No hazard statement andthe signal word are required.
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