Tetrasodium 3,3'-[carbonylbis[imino(5-methoxy-2-methyl-4,1-phenylene)azo]]bis(naphthalene-1,5-disulphonate)

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

06 February 2017 to 08 March 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

- Concentrations: in the main test controls and at 100 mg/L at 0 hour (bulk) and 48 hour (pooled from replicates) in duplicate
- Sample storage conditions before analysis:frozen

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION : A nominal amount of test item (100 mg) was dissolved in a 1000 mL volume of test water with the aid of sonication for approximately 5 minutes to give the 100 mg/L test concentration.
The prepared concentration was inverted several times to ensure adequate mixing and homogeneity.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Source: in-house laboratory culture
- Age : < 24 hours
- Feeding during test: none

ACCLIMATION
- Acclimation period: NA
- Culture conditions: temperature 18 to 22 °C; 16 hours light and 8 hours darkness cycle with 20 minute dawn and dusk transition periods.:
- Type and amount of food: algal suspension (Desmodesmus subspicatus) and Tetramin flake food suspensio
- Feeding frequency: daily

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

NA

Test temperature:

19-22 ºC

pH:

7.7-7.8

Dissolved oxygen:

8.5-9.2 mg/L (102-108% of saturation)

Salinity:

NA

Nominal and measured concentrations:

nominal: 0 and 100 mg/L
measured (at 0 and 48 h):

Details on test conditions:

TEST SYSTEM
- Test vessel: 150 mL glass beakers with 100 mL test preparation
- Type: closed
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: fresh water (Elendt M7 medium according to the guideline pH 7.9, kept at 21 ºC)
- Adjustment of pH: no
- Photoperiod: 16 h light
- Light intensity: 386 to 568 Lux

EFFECT PARAMETERS MEASURED: immobility:

RANGE-FINDING STUDY
- Test concentrations: 0, 0.1, 1, 10 and 100 mg/L
- Results used to determine the conditions for the definitive study:no immobilisation at any of the concentrations tested

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: measured concentrations >80% of nominal

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: measured concentrations >80% of nominal

Results with reference substance (positive control):

potassium dichromate EC50 (48 h) 1.2 mg/L

Reported statistics and error estimates:

NA

Validity criteria fulfilled:

yes

Conclusions:

The 48 h EC50 for daphnia magna is >100 mg/L

Executive summary:

In a static test according to OECD 202 20 daphnids/concentration were exposed to 0 and 100 mg/L in Elendt M7 medium. Measured concentrations at 0 and 100 mg/L were at 0 and 48 h: <LOQ and < LOQ, and 98.0 and 96.3 mg/L. No immobility was observed. The 48 h EC50 for daphnia magna is > 100 mg/L.

Description of key information

In a static test according to OECD 202 20 daphnids/concentration were exposed to 0 and 100 mg/L in Elendt M7 medium. Measured concentrations at 0 and 100 mg/L were at 0 and 48 h: <LOQ and < LOQ, and 98.0 and 96.3 mg/L. No immobility was observed. The 48 h EC50 for daphnia magna is > 100 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L