Aesculin

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

2004

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

Analytical data are required by the guidelines for verification of the test item concentration as well as the stability of the test item over the entire test period. Analytical samples were taken from the test item concentration and control at test start from fresh solutions and after 48 hours from aged solutions. For each sampling also a retain sample was taken.

Vehicle:

no

Details on test solutions:

not applicable

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna Straus, Clone V, was used as the test organism. The animals are continuously bred in the laboratory and were originally purchased in a healthy condition from the Federal Environment Agency in Berlin/Germany. Daphnia magna was bred as single culture (1 daphnid per 100 mL) in Elendt M4 medium. The pHvalue of the aerated water was within a range of 6.0 – 9.0. The dissolved oxygen was above 60 % saturation and the total hardness 140 - 250 mg/L (as CaCO3), corresponding to 7.8 - 14°dH. The animals were fed with single cell green algae (Desmodesmus subspicatus, formerly Scenedesmus subspicatus) at least three times a week.
The daphnids were reared at a temperature of 20 ± 2 °C in a climatic chamber with 16 hours of illumination and 8 hours of darkness. The medium was changed three times per week. A pipette was used to separate the young daphnids from the adults.
Freshly hatched daphnids less than 24 hours old were used for the test.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Remarks on exposure duration:

none

Post exposure observation period:

no

Hardness:

The total hardness (as CaCO3) of the untreated control was determined to be 13°dH (232 mg/L CaCO3); the mean pH-value of the untreated control was determined to be 7.92 ± 0.17 (Std. Dev.), the mean temperature of the control and 100 mg/L test item was measured to be 20.2 ± 0.9 °C (Std. Dev.) and the mean oxygen concentration was determined to be 8.9 ± 0.4 mg/L (Std. Dev.).

Test temperature:

20.2 ± 0.9 °C

pH:

7.92 ± 0.17

Dissolved oxygen:

8.9 ± 0.4 mg/L

Salinity:

not reported

Conductivity:

not reported

Nominal and measured concentrations:

The initial measured content of active ingredient was 100 % of nominal and the aged measured content was 98 % of nominal. Since the content of active ingredient in the samples was between 80 and 120 % of nominal the toxicological endpoints were evaluated using nominal concentrations.

Details on test conditions:

The total hardness (as CaCO3) of the untreated control was determined to be 13°dH (232 mg/L CaCO3); the mean pH-value of the untreated control was determined to be 7.92 ± 0.17 (Std. Dev.), the mean temperature of the control and 100 mg/L test item was measured to be 20.2 ± 0.9 °C (Std. Dev.) and the mean oxygen concentration was determined to be 8.9 ± 0.4 mg/L (Std. Dev.).

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: n.d.

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: n.d.

Key result

Duration:

24 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: n.d.

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: n.d.

Validity criteria fulfilled:

yes

Conclusions:

Aesculin is not harmfull to Daphnia under the conditions used

Executive summary:

According to the OECD Guideline No. 202 (2004) the EC50 (48 h) was determined to be >100 mg/l (nominal) for the test substance AESCULIN and therefore AESCULIN has to be classified to be not harmful to Daphnia.

Description of key information

Aesculin was considered to be non harmful to aquatic invertebrates based on the tests performed with Daphnia magna

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

According to the results of the test, the EC50 (48h) for immobilisation was determined to be

>100mg/L(nominal).The corresponding NOEC(48 h) was 100mg/L (nominal).