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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2018
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
2004
Deviations:
no
GLP compliance:
yes (incl. certificate)
Analytical monitoring:
yes
Details on sampling:
Analytical data are required by the guidelines for verification of the test item concentration as well as the stability of the test item over the entire test period. Analytical samples were taken from the test item concentration and control at test start from fresh solutions and after 48 hours from aged solutions. For each sampling also a retain sample was taken.
Vehicle:
no
Details on test solutions:
not applicable
Test organisms (species):
Daphnia magna
Details on test organisms:
Daphnia magna Straus, Clone V, was used as the test organism. The animals are continuously bred in the laboratory and were originally purchased in a healthy condition from the Federal Environment Agency in Berlin/Germany. Daphnia magna was bred as single culture (1 daphnid per 100 mL) in Elendt M4 medium. The pHvalue of the aerated water was within a range of 6.0 – 9.0. The dissolved oxygen was above 60 % saturation and the total hardness 140 - 250 mg/L (as CaCO3), corresponding to 7.8 - 14°dH. The animals were fed with single cell green algae (Desmodesmus subspicatus, formerly Scenedesmus subspicatus) at least three times a week.
The daphnids were reared at a temperature of 20 ± 2 °C in a climatic chamber with 16 hours of illumination and 8 hours of darkness. The medium was changed three times per week. A pipette was used to separate the young daphnids from the adults.
Freshly hatched daphnids less than 24 hours old were used for the test.
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Remarks on exposure duration:
none
Post exposure observation period:
no
Hardness:
The total hardness (as CaCO3) of the untreated control was determined to be 13°dH (232 mg/L CaCO3); the mean pH-value of the untreated control was determined to be 7.92 ± 0.17 (Std. Dev.), the mean temperature of the control and 100 mg/L test item was measured to be 20.2 ± 0.9 °C (Std. Dev.) and the mean oxygen concentration was determined to be 8.9 ± 0.4 mg/L (Std. Dev.).
Test temperature:
20.2 ± 0.9 °C
pH:
7.92 ± 0.17
Dissolved oxygen:
8.9 ± 0.4 mg/L
Salinity:
not reported
Conductivity:
not reported
Nominal and measured concentrations:
The initial measured content of active ingredient was 100 % of nominal and the aged measured content was 98 % of nominal. Since the content of active ingredient in the samples was between 80 and 120 % of nominal the toxicological endpoints were evaluated using nominal concentrations.
Details on test conditions:
The total hardness (as CaCO3) of the untreated control was determined to be 13°dH (232 mg/L CaCO3); the mean pH-value of the untreated control was determined to be 7.92 ± 0.17 (Std. Dev.), the mean temperature of the control and 100 mg/L test item was measured to be 20.2 ± 0.9 °C (Std. Dev.) and the mean oxygen concentration was determined to be 8.9 ± 0.4 mg/L (Std. Dev.).
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Key result
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: n.d.
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: n.d.
Key result
Duration:
24 h
Dose descriptor:
NOEC
Effect conc.:
>= 100 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: n.d.
Key result
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
>= 100 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: n.d.
Validity criteria fulfilled:
yes
Conclusions:
Aesculin is not harmfull to Daphnia under the conditions used
Executive summary:

According to the OECD Guideline No. 202 (2004) the EC50 (48 h) was determined to be >100 mg/l (nominal) for the test substance AESCULIN and therefore AESCULIN has to be classified to be not harmful to Daphnia.

Description of key information

Aesculin was considered to be non harmful to aquatic invertebrates based on the tests performed with Daphnia magna

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
100 mg/L

Additional information

According to the results of the test, the EC50 (48h) for immobilisation was determined to be

>100mg/L(nominal).The corresponding NOEC(48 h) was 100mg/L (nominal).