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EC number: 227-029-3 | CAS number: 5610-64-0 This substance is identified in the Colour Index by Colour Index Constitution Number, C.I. 15711.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study following official guideline not GLP compliant
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- GLP compliance:
- no
- Limit test:
- yes
Test material
- Reference substance name:
- Acid Black 52
- IUPAC Name:
- Acid Black 52
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- Source: breeding farm VELAZ
Sex: males
Acclimatisation: minimum 5 days
Total number: 5
Housing: animal room with monitored conditions-5 animals in one plastic breeding cage Velaz T4
Diet: standard pelleted diet
Water: drinking tap water ad libitum
Microclimatic conditions: room temperature 22 +/- 3°C permanently monitored
relative humidity 30-70% permanently monitored
light: 12 hours light/12 hours dark
Bedding: sterilized shavings of soft wood
Randomisation: according to the internal rule, at the start of the study the weight variation of animals was minimal and did not exceed +/- 20% fi the mean weight for each sex
Identification of animals: colour marks 1-5 on tail of animals, each cage was marked with the number of study, sex and dose of the test substance
Health conditions: certificate of good health condition-from breeding farm; no signs of diseases were observed at clinical check-in, during the acclimatisation period and before the strat of study
Administration / exposure
- Type of coverage:
- occlusive
- Vehicle:
- other: moistened with water pro-injection
- Details on dermal exposure:
- The amount of the test substance for each animal was weighed 8according to its body weight) and moistened with water for injection immediately before application.
Aproximately 24h before application the fur on the back of animals was shaved at area about 6x6 cm. Aliquot part of the test substance was applied on the depilated area of skin. The application site was covered by mull, plastic foil and held in contact by plaster (strapping). After 24h the occlusive dressing and the remains of test substance were removed. - Duration of exposure:
- 24h
- Doses:
- 5000 mg/kg bw
- No. of animals per sex per dose:
- 5 males
- Control animals:
- no
- Details on study design:
- BODY WEIGHT RECORDING
Animlas were weighed before application, at day 8 and before euthanasia of animals. Average body weight in a group was calculated from individual weights.
CLINICAL EXAMINATIONS
After application the animals were observed individually
1st day: at 30 min and 3h
2nd day: in the morning and in the afternoon
up to 14th day: once daily
Observations included: changes in the skin and fur, eyes, visible mucous membranes, behaviour of animals, somatomotor activity, reactions to stimuli, presence of lacrimation, salivation and discharge from nostrils, function of respiratory, digestive and urogenital system.
PATHOLOGICAL EXAMINATION
All the test animals that survived to the end of the study were sacrificed on the 15th day and gross necroscopy was performed. Nutritious status, body surface, body foramina, thoracic, abdominal and cranial cavity were evaluated.
Results and discussion
Effect levels
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 5 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- No mortality
- Clinical signs:
- other: No clinical signs
- Gross pathology:
- All animals were without patho-morfological signs
Applicant's summary and conclusion
- Interpretation of results:
- other: not classified under Regulation 1272/2008
- Conclusions:
- The substance was tested for acute toxicity dermal, in a limit test, following OECD 402. The LD50 (LD0) was found to be equal to 5000 mg/kg bw
- Executive summary:
The substance was tested for acute toxicity dermal, in a limit test, following OECD 402. The LD50 (LD0) was found to be equal to 5000 mg/kg bw
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