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EC number: 700-502-6 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2008-11-19
- Reliability:
- 1 (reliable without restriction)
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2009
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- no
- Principles of method if other than guideline:
- not applicable
- GLP compliance:
- yes (incl. QA statement)
Test material
Reference
- Name:
- Unnamed
- Type:
- Constituent
- Details on test material:
- not applicable
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
not applicable
Sampling and analysis
- Analytical monitoring:
- not required
- Details on sampling:
- not applicable
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Before the start of the test defined amounts of the test item were directly weighed into the test flasks and mechanically dispersed using short ultrasonic bath treatment for 10 min. The test concentrations (10, 31, 100, 313 and 1000 mg/L) were chosen to permit the determination of the EC50. Concentrations in excess of nominal 1000 mg test item/L were not tested. Two controls (deionised water, synthetic sewage and inoculum, but without addition of the test item) were tested in parallel.
Test organisms
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- The activated sludge used for this study was washed and centrifuged and the supernatant liquid phase was decanted. The solid material was re-suspended in isotonic saline solution and again centrifuged. This procedure was repeated twice. An aliquot of the final sludge suspension was weighed, dried and a ratio of wet sludge to its dry weight determined. Based on this ratio, calculated amounts of wet sludge were suspended in isotonic saline solution to yield a concentration equivalent to 4 g per litre (on dry weight basis). The pH of the activated sludge inoculum was determined to be pH 7.14. The activated sludge was used directly after conditioning. The activated sludge was supplied from the sewage plant for domestic sewage in Veszprém, Hungary.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- not applicable
Test conditions
- Hardness:
- not applicable
- Test temperature:
- 19.7 – 20.6 C (during the incubation)
18.6 – 20.6 C (during oxygen measurement) - pH:
- The pH of the activated sludge inoculum was determined to be pH 7.14
- Dissolved oxygen:
- The concentration of dissolved oxygen did not drop below 2.5 mg O2/L during the incubation period, and just before the measurements of the respiration rates the oxygen concentrations were at least 7.5 mg O2/L.
- Salinity:
- not applicable
- Nominal and measured concentrations:
- nominal: 10, 31, 100, 313 and 1000 mg/L
no concentrations measured - Details on test conditions:
- aerobic incubation
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- The NOEC for respiration rates was not based on the results of a statistical analysis, but it is biologically justified. The respiration rates were not inhibited and influenced dose dependently in the whole concentration range, and the observed slight inhibition (13.0 %) at the highest examined concentration level of 1000 mg/L was evaluated as reflecting the biological variability in the test. The calculated respiration rate, 0.470 (at 1000 mg/L) was in the historical control data range (0.513 ± 0.079), and the deviation from the control was within ± 15 %, can be considered as a biological variability of the test system.
- Results with reference substance (positive control):
- The 3-hour EC50 of the reference item 3,5-Dichlorophenol for the used activated sludge batch was determined to be 8.94 mg/L with 95 % confidence limits of 7.17 to 11.14 mg/L and thus the EC50 was in the acceptable the range of 5 to 30 mg/L.
- Reported statistics and error estimates:
- not applicable
Any other information on results incl. tables
no remarks
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- EC50 (3h) > 1000 mg/l
- Executive summary:
In comparison to the inoculum controls the respiration rate of the activated sludge was not inhibited in the concentration range of 10 to 1000 mg F 213 Red /L. The respiration rate was inhibited between 1.9 % (10 mg F 213 Red/ L) and 13.0 % (1000 mg F 213 Red/ L) in the examined nominal test concentration range. Concentrations exceeding 1000 mg/L nominal were not tested.
NOEC (3h) and EC50 (3h) > 1000 mg/l
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