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EC number: 801-277-8 | CAS number: 507448-65-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 2000
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- ethyl (2Z)-4,4,4-trifluoro-3-(methylamino)but-2-enoate
- EC Number:
- 801-277-8
- Cas Number:
- 507448-65-9
- Molecular formula:
- C7H10F3NO2
- IUPAC Name:
- ethyl (2Z)-4,4,4-trifluoro-3-(methylamino)but-2-enoate
Constituent 1
Test solutions
- Details on test solutions:
Stock solution:
200.5 mg test item were mixed (polytron for 1 minute) and made up to 1000 ml with water.
The stock solution was freshly prepared at the start of the test.
Test concentrations:
Test item: 100.0, 32.0, 10.0, 3.2 and 1.0 mg/L
Reference item: 32.0, 10.0 and 3.2 mg/L
Calculated amounts of the test item to produce the desired test concentrations were mixed with 114 ml water and 6.4 ml nutrient solution (= test solution). Then 80 ml of the sludge were added.
The test item, 6.4 ml of the nutrient solution, 114 ml water and 80 ml of the sludge were mixed in 250 ml BOD flasks and aerated immediately.
Test organisms
- Test organisms (species):
- activated sludge
- Details on inoculum:
- Source: Activated sludge collected from the sewage treatment plant of CH-4153 Reinach on November 3, 1999.
- pH at collection: The pH after collection was 7.7.
- Preparation: The preparation was carried out according to the method in the guideline.
- Separation method: The sludge was separated from the aqueous layer by settling instead of centrifugation.
- pH before use: The pH of the sludge before use was 7.8.
- Concentration: The sludge concentration in the test bottles was 1.71 g suspended solids/L.
Study design
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- 20 ± 2 °C
- pH:
- The pH of sludge was 7.7 after collection and 7.8 after preparation (before use).
The pH of the mixtures in the test bottles was 8.2 – 8.3 at the end of exposure time. - Nominal and measured concentrations:
- Test concentrations:
Test item: 100.0, 32.0, 10.0, 3.2 and 1.0 mg/L
Reference item: 32.0, 10.0 and 3.2 mg/L - Details on test conditions:
- Vessels: 250 ml BOD flasks with gas inlet
- Water: De-chlorinated drinking water
- Temperature: 20 ± 2 °C
- Duration: 3 hours.
Measurements:
Oxygen consumption per hour in mg/L with a ORION – Electrode Type 97-08, ORION Microprocessor Ionalizer 901 and plotted on a recorder.
Calculations:
The inhibitory values were calculated on the basis of the measured time dependant oxygen consumption of a blank and test solution.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: respitation inhibition
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: respiration inhibition
- Duration:
- 3 h
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: respiration inhibition
- Details on results:
The inhibitory effect of the test item was expressed as a percentage of the mean respiration rates of two controls. Following values were calculated based on nominal concentrations:
The test item CA 2455 A was tested at nominal concentrations of 1.0, 3.2, 10.0, 32.0 and 100.0 mg test item/L after a contact time of 3 hours. The calculation of the following effect concentrations was based on these nominal values.
EC50 (3h) : > 100 mg/L
EC20 (3h) : > 100 mg/L
EC80 (3h) : > 100 mg/L
For the reference item (tested nominal concentrations) 3.2, 10 and 32 mg/L:
EC50 (3h) : 10.5 mg/L
EC20 (3h) : 3.8 mg/L
EC80 (3h) : 29.0 mg/L
Test Validity;
The deviation of the respiration rates in controls was 4 %. The EC 50 (3h) of the reference item was 10.5 mg/L. Since the deviation of the respiration rates of the controls was < 15 % and the EC 50 (3h) of the reference item was in the range of 5 to 30 mg/L, the test was valid in terms of the given validity criteria.
Conclusion:
The EC50 (3h) for CA 2455 A is > 100 mg/L. Based on the inherent toxicity of CA 2455 A in this test alone, the test item can be classified as: “not toxic to bacteria”.- Results with reference substance (positive control):
- Refer to above results section.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC 50 (3h) for CA 2455 A is > 100 mg/L.
Based on the inherent toxicity of CA 2455 A in this test alone, the test item can be classified as:
“not toxic to bacteria” - Executive summary:
The respiration rate of an activated sludge fed with standard amount of synthetic sewage feed was measured after a contact time of 3 hours. The respiration rate of the same activated sludge was also measured in the presence of 1.0, 3.2, 10.0, 32.0 and 100 mg test item/L and 3.2, 10 and 32 mg/L reference item, respectively.
The sludge concentration in the test bottles was 1.71 g suspended solids/L.
The test with CA 2455 A the temperature was maintained constant at 20 ± 1°C.
The pH of sludge was 7.7 after collection and 7.8 after preparation (before use).
The pH of the mixtures in the test bottles was 8.2 – 8.3 at the end of exposure time.
The EC50 (3h) of the reference item 3,5-Dichlorophenol was 10.5 mg/L
The inhibitory effect of the test item was expressed as a percentage of the mean respiration rates of two controls. Following values were calculated based on nominal concentrations:
EC50 (3h) : > 100 mg/L
EC20 (3h) : > 100 mg/L
EC80 (3h) : > 100 mg/L
Conclusion:
The EC 50 (3h) for CA 2455 A is > 100 mg/L.
Based on the inherent toxicity of CA 2455 A in this test alone, the test item can be classified as:
“not toxic to bacteria”
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