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EC number: 700-626-0 | CAS number: 1073607-50-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Qualifier:
- according to guideline
- Guideline:
- ISO DIS 9439 (Ultimate Aerobic Biodegradability - Method by Analysis of Released Carbon Dioxide)
- Qualifier:
- according to guideline
- Guideline:
- other: ISO Standard 10634 "Water Quality - Guidance for the preparation and treatment of poorly water-soluble organic compounds for the subsequent evaluation of their biodegradability in an aqueous medium" (1995)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2-[(1E)-2-[(3Z)-2-chloro-3-{2-[(2E)-1,1-dimethyl-3-(4-sulfobutyl)-1H,2H,3H-benzo[e]indol-2-ylidene]ethylidene}cyclohex-1-en-1-yl]ethenyl]-1,1-dimethyl-3-(4-sulfonatobutyl)-1H-benzo[e]indol-3-ium amine
- EC Number:
- 700-626-0
- Cas Number:
- 1073607-50-7
- Molecular formula:
- C46 H50 Cl N2 O6 S2 . H4 N
- IUPAC Name:
- 2-[(1E)-2-[(3Z)-2-chloro-3-{2-[(2E)-1,1-dimethyl-3-(4-sulfobutyl)-1H,2H,3H-benzo[e]indol-2-ylidene]ethylidene}cyclohex-1-en-1-yl]ethenyl]-1,1-dimethyl-3-(4-sulfonatobutyl)-1H-benzo[e]indol-3-ium amine
- Details on test material:
- Since the test substance was not sufficiently soluble to allow preparation of an aqueous solution at a concentration of 1 g/l, weighed amounts were added to the 2-litres test bottles containing medium with microbial organisms and mineral components. To this end, 20 ml of Milli-RO water was added to each weighing bottle containing the test substance. After vigorous mixing (vortex) the resulting suspension was added quantitatively to the test medium. The test solutions were continuously stirred during the test, to ensure optimal contact between the test substance and the test organisms.
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- The freshly obtained sludge was kept under continuous aeration until further treatment. The concentration of suspended solids was 3.4g/l in the concentrated sludge (information obtained from the municipal sewage treatment plan). Before use, the sludge was allowed to settle (32 minutes) and the supernatant liquid was used as inoculum at the amount of 10ml/l of mineral medium.
- Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 18.5 mg/L
- Based on:
- other: corresponding to 12mg TOC/L
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- The study consisted of six bottles:
- 2 inoculum blanks (no test substance)
- 2 test bottles (test substance)
- 1 positive control (sodium acetate)
- 1 toxicity control (test substance + sodium acetate)
Reference substance
- Reference substance:
- acetic acid, sodium salt
- Remarks:
- positive control and part of toxicity control
Results and discussion
- Test performance:
- 1. temperature: The temperature recorded in a vessel with water in the same room varied between 21.9 and 22.9°C.
2. pH:
inoculum blanks: 7.5 (start) 7.6 (day 28)
positive control: 7.5 (start) 7.8 (day 28)
toxicity control: 7.5 (start) 7.8 (day 28)
test substance: 7.5 (start) 7.6 (day 28)
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Value:
- >= 18 - <= 19
- St. dev.:
- 1
- Remarks on result:
- other: Biodegradation was determined on day 2, 5, 7, 9, 14, 19, 23, 27 and 29.
- Details on results:
- In the toxicity control, the test substance was found not to inhibit microbial activity (more than 25% biodegradation occurred withiin 14 days)..
Any other information on results incl. tables
Acceptability of the test:
1. The positive control substance was biodegraded by at least 60% (76%) within 14 days.
2. The difference of duplicate values for %-degradation of the test substance was always less than 20.
3. The total CO2 release in the blank at the end of the test did not exceed 40 mg/l (52.5 mg CO2 per 2 litres of medium, corresponding to 26.3 mg CO2/l).
4. The Inorganic Carbon content (IC) of the test substance (suspension) in the mineral medium at the beginning of the test was less than 5% of the Total Carbon content (TC). Since the test medium was prepared in tap-water purified by reverse osmosis (Milli-RO water (Millipore Corp., Bedford, Mass., USA, carbon levels < 500 ppb)), IC was less than 5% of TC (mainly coming from
the test substance, 12 mg TOC/l).
Since all criteria for acceptability of the test were met, this study was considered to be valid.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other:
- Conclusions:
- Not readily biodegradable
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