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EC number: 212-001-5 | CAS number: 736-30-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: well performed GLP and OECD guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 987
- Report date:
- 1987
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- 1,1'-ethane-1,2-diylbis(4-nitrobenzene)
- EC Number:
- 212-001-5
- Cas Number:
- 736-30-1
- Molecular formula:
- C14H12N2O4
- IUPAC Name:
- 1,1'-ethane-1,2-diylbis(4-nitrobenzene)
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Strain: Sprague-Dawley CFY
- Source: Interfauna (UK) Limited, Wyton, Huntingdon, Cambridgeshire
- Age at study initiation: 5 - 8 weeks
- Weight at study initiation: males weighed 160 - 182g, females 148 - 179g
- Fasting period before study: overnight before study
- Housing: up to five by sex in solid-floor polypropylene cages with sawdust bedding.
- Diet: Rat and Mouse Expanded Diet No. 1, Special Diet Services Limited, Witham, Essex, U.K ad libitum
- Water: tap water ad libitum
- Acclimation period: at leat 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 - 22
- Humidity (%): 55 - 68
- Air changes (per hr): approximately 15
- Photoperiod: controlled by a time switch to give 12 hours light and 12 hours darkness.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- arachis oil
- Details on oral exposure:
DOSE LEVEL CONCENTRATION DOSE VOLUME NUMBER OF RATS
mg/kg mg/kg ml/kg MALE FEMALE
Range finding: 5000 500 10 1 1
Range finding: 2000 200 10 1 1
Main Study: 5000 500 10 5 5- Doses:
DOSE LEVEL CONCENTRATION DOSE VOLUME NUMBER OF RATS
mg/kg mg/kg ml/kg MALE FEMALE
Range finding: 5000 500 10 1 1
Range finding: 2000 200 10 1 1
Main Study: 5000 500 10 5 5- No. of animals per sex per dose:
- Range finding: 1
Main Study: 5 - Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations: 1 and 4 hours after dosing and subsequently once daily for 14 days.
- Frequency of weighing: day 0, 7 and 14
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight,
Results and discussion
- Preliminary study:
- Range finder 5000 mg/kg bw and 2000 mg/kg bw with one male and one female for each concentration. No mortality
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 5 000 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: no deathes occured
- Mortality:
- no mortality occured
- Clinical signs:
- other: No signs of systemic toxicity were noted during the study period
- Gross pathology:
- No abnormalities were noted at necropsy of animals killed at the end of the study
Applicant's summary and conclusion
- Interpretation of results:
- not classified
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The acute oral median lethal dose (LD50 ) of DNBY, in the rat was found to be greater than S000 mg/kg bodyweight.
Therefore, the substance is not subject for classification for acute oral toxicity. - Executive summary:
The study was performed to assess the acute oral toxicity of the test material in the rat. The study was based on the recommendations of the OECD Guidelines for Testing of Chemicals (1981) No. 401 "Acute Oral Toxicity". For the purpose of this study the test material was ground to a fine powder using a mortar and pestle and freshly prepared, as required, at the appropriate concentrations in arachis oil B.P. All rats were dosed once only by gavage using a metal cannula attached to a graduated syringe.
A preliminary study was performed using pre-selected dose levels to determine the highest of these dose levels that produced a mortality rate of less than 50% with 5000 and 2000 mg/kg bw using one male and one female animal for each concentration.
For the main study 5 male and 5 female animals were administered with a single dose of 5000 mg/kg bw.
Animals were observed 1 and 4 hours after dosing and subsequently once daily for 14 days.
No mortality occured. No signs of systemic toxicity were noted during the study period. All animals showed expected gains in bodyweight over the study period. No abnormalities were noted at necropsy of animals killed at the end of the study.
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