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reaction product of: saturated, monounsaturated and multiple unsaturated long-chained partly estrified alcohols of vegetable origin (Brassica napus L., Brassica rapa L., Helianthus annuus L., Glycine hispida, Gossypium hirsutum L., Cocos nucifera L., Elaeis guineensis) with O,O-diisobutyldithiophosphate and 2-ethylhexylamine and hydrogen peroxide
EC number: 428-630-5 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 11/08/1993 to 11/23/1993
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP study conducted in compliance with agreed test protocols and guidance; restriction because the procedures were similar but different from OECD 209, such as test organisms exposed to test sample for prolonged exposure period.
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 991
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- The test was performed according to the guideline DIN 38412, part L8 (March 1991).
- Deviations:
- no
- Principles of method if other than guideline:
- not applicable.
- GLP compliance:
- yes
Test material
- Reference substance name:
- -
- EC Number:
- 428-630-5
- EC Name:
- -
- Molecular formula:
- Not applicable
- IUPAC Name:
- reaction product of Z-9-octadecen-1-ol and O,O-diisobutyl hydrogen dithiophosphate
- Test material form:
- other: liquid
- Details on test material:
- - Physical state: liquid
- Storage condition of test material: room temperature, protected from light
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
none
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- To reach a maximum nominal concentration of 10 g/l in the test, a stock emulsion containing 12.5 g/l of the test article was p~pared.A stock emulsion containing 1 g/l of the test article was prepared in the presence of the emulgator Tween 80. Following stirring overnight at room temperature, homogenous emulsions were obtained. These were filte d through paper filters and samples were analysed by DOC measurement. The Doc was determined as the result from subtracting the values for TOC (total organic carbon) and IC (inorganic carbon). Analysis was performed at least in duplicate. The DOC content of the stock emulsion containing 1 g/l of the test article in the presence of Tween 80 (100 mg/l) was corrected by the DOC of purified water, containing Tween 80 (100 mg/l).
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The main test was performed with a series of five nominal concentrations of the two stock emulsions prepared with the test article, each concentration being tested in triplicate. The highest concentration represented nominal concentrations of 10 g/l and the lowest of 50 mg/l of the test article. The nominal test concentrations were obtained by adding appropriate volumes of the test article stock emulsions, containing 12.5 g/l and 1 g/l (in the presence of Tween 80, 100 mg/l) of the test article.
Test organisms
- Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- Pseudomonas purida, DSM 50026 (lyophilisate, obtained March 1993)
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 16 h
- Post exposure observation period:
- none
Test conditions
- Hardness:
- no data
- Test temperature:
- 21 +/- 1°C
- pH:
- The pH-value of the cultures containing the highest nominal concentrations of the test article (10000mg/l) was 7.3.
- Dissolved oxygen:
- no data
- Salinity:
- not applicable
- Nominal and measured concentrations:
- Aqueous stock emulsion containing 1.0 g/l (nominal) test article and with TWEEN 80 (100 m/l): 800, 400, 200, 100, 50 mg/l
Aqueous stock emulsion containing 12.5 g/l (nominal) test article: 10, 5.0, 2.5, 1225, 0.6 g/l
Aqueous stock emulsion containing 0.1 g/l (nominal) test article: 0.05, 0.1, 0.2, 0.4, 0.8 g/l - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 ml Erlenmeyer flasks
- No. of organisms per vessel: no data
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 3
- Biomass loading rate: no data
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: in the dark
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2 - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol (97% pure)
Results and discussion
Effect concentrations
- Duration:
- 16 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Details on results:
- Control cultures were treated accordingly with the exception that they contained appropriate volumes of purified water (or purified water in the presence of 100 mg/l of the emulsifier) instead of the test article. The cell multiplication of the concurrent control cultures met validity criteria
In order to confm the functional integrity of the test system, the reference substance 3,5-dichlorophenol was used in control tests performed on a regular basis. The EC50 and EC10 values obtained were comparable to those stated in DIN 38412 L8. Thus, in terms of sensitivity, the experimental system meets the requirements of the guideline.
With an aqueous emulsion of the test article in the absence of Tween 80, values of EC10 and EC50 could not be calculated, because maximum inhibition of 24% was obtained with the highest nominal concentration tested (10.0 g/l).
The EC10 was determined to be 0.08 g/l, and the EC50 was determined to be 0.15 g/l of the test article, when emulsified in the presence of Tween 80, which supported solubility / bioavailability of test article. - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: EC50(16h) = 17.3 mg/L; EC10(16h) = 6.5 mg/L;
According to the guideline DIN 38412 L8, the mpective values for this substance obtained in a series of interlaboratory comparison experiments were 21.4 +/- 4.91 mg/l and 13.7 +/- 4.36 mg/l. - Reported statistics and error estimates:
- none
Any other information on results incl. tables
The turbidity of the test culturesforeach concentration of the test articleandthat of the control cultureswithin the preliminary test arepresented in Table1.Inhibitory effects were observed at nominal concentrations >100mg/l.
The turbidity of the test culturesforeach concentration ofthetest articleandthat of the control cultureswithin the main test arepresented in Table2.
Thecorresponding values fortheinhibition of cell multiplication compiled in Table 3.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- With an aqueous emulsion of the test article in the absence of Tween 80, it may be stated that the EC50 is achieved at a concentration > 10.0 g/l.
- Executive summary:
Guidance
Theaimofthisstudy was toassessthe potential inhibitory effect of the test article on thegrowthofPseudonroMs putida:MIGULA (DSM50026). Test wasperformed according tothe guideline DIN38412,partL8 (March 1991).
Material and Method
Adescending range of concentrations of the test article Folpan was assayed for potential inhibitory effects on thegrowthof Pseudomonasputida.Accordingtothe recommendations of the "Kommission Bewertung Wassergef Wnder Stoffe", stock emulsions of12.5g/land 100 mg/l were prepared. Additionally,astock emulsion of 1.0g/lwas prepared in the presence ofanemulsifier Tween80, 100mg/l).
RESULTS
Within the preliminary test, inhibitory effects were observed at or above nominal concentrations of5.0g/lusing the filtered phases of the stock emulsions of12.5g/l.However, inhibitory effects were observed at or above concentrations of200mg/lwhen using a stock solution of1 g/lin the presence of the emulsifier Tween80.
Negative inhibition values indicateanenhancement ofcellmultiplication as compared to control cultures, whilst positive values indicateanimbibition of growth relativetocontrol cultures.
Theresults of thepreliminarytest were confirmed by the main test,performedwith descending concentrations ofthefiltered phases of thestockemulsions containing nominal concentrations of 12.5g/land1g/lin the presence of the emulsifier Tween80.A maximum inhibition of 24% was obtained with the highest nominal concentrationtested(10.0 g/l) whereas clear inhibitory effects were observed atorabove concentrations of0.05mg/l when using astocksolution of 1g/l,prepared inthe presence of the emulsifier Tween80.This effect demonstrates the influence of Tween80,i.e. in supporting solubility bioavailability of potential toxic compounds.
The EC10wasdetermined to be 0.04g/l,and the EC50wasdetermined to be 0.15g/lof the test article, when emulsified in the presence of Tween80.
Withanaqueous emulsion of the test article in the absence of Tween80,it maybestated that the EC50 is achieved ataconcentration > 10.0g/l.
The pH-value of the culture containing the highest nominal concentration of the test article(10000mg/l) was7.3at initiation of the test.
Aqueous stock emulsions of12.5g/land 1g/l(in presence of the emulsifier Tween 80, 100mg/l)of the test article contained values of 920 mgC/land67mgC/l, respectively. The DOC of the stock emulsion containing Tween 80 was correctedforthe DOC of purified water, containing Tween 80(100mg/l).
The mean biomass of the strain Pseudomonasputida,determined from the turbidity of theculturesat the beginning and end of the 16 +/-1 h- incubation periodofthe maintest,increased by more than 100-fold from 1.9to194. Therefore, the criterionofvalidityof thistest was fulfilled.
Within the last representative referencetest(performed between the 21St and 23dofJuly,
1993) with 3-dichlorophenol,testedunder the same conditions, pronounced inhibitory effects on cellgrowthwas documented, whichresultedinEC50andEC10values of 17.3 and6.5mg/l,respectively.Accordingtothe guideline DIN 38412L8,the respective values forthis substanceobtained in a seriesof interlaboratorycomparison experiments were 21.4 +/-4.91 mg/ and 13.7 +/-4.36 mg/l.
CONCLUSIONS
With an aqueous emulsion of the test article in the absence of Tween80,it maybestated that the EC50 is achieved ataconcentration > 10.0g/l.
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