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EC number: 627-872-0 | CAS number: 1514-82-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 17 October 2012 - 05 October 2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0 (dilution medium control), 6.25, 12.5, 25, 50, and 100 mg/L
At the start of the definitive test, specific analytical vessels were prepared in the same manner as the exposure vessels (100 mL media in a 250 mL vessel with 150 mL
headspace). Freshly prepared control and test media was analysed on Day 0. After 48 hours, media in specified vessels prepared on Day 0 and left under test conditions for the duration of the definitive test, were analysed. The vessels specified for analysis did not contain test organisms.
Samples were extracted into hexane using liquid-liduid extraction (10 mL sample shaken with 10 mL of hexane). - Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Strain: Straus
- Age at study initiation: less than 24 hours old
- Method of breeding: parthenogenesis - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 180 mg/L as CaCO3
- Test temperature:
- 20.2 - 20.4°C
- pH:
- 7.75 - 7.85
- Dissolved oxygen:
- 96 - 100% ASV (Air saturation value)
- Nominal and measured concentrations:
- Nominal: 0 (Control), 6.25, 12.5, 25, 50, 100 mg/L
Initial measured: 0 (no test material detected), 2.45, 4.63, 11.1, 21.9, 42.9 mg/L
Measured concentrations after 48 hours: 0, 0.194, 0.390, 0.752, 1.86, 3.56 mg/L
Geometric mean concentration (initial and 48-hour values): 0, 0.7, 1.3, 2.9, 6.4, 12.4 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel:250 mL glass bottles. Fill volume = 100 mL, headspace = 150 mL. Bottles were sealed after addition of the test material
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 20 mL medium per organism
TEST MEDIUM / WATER PARAMETERS
Softened Elendt M4 medium to comply with Method 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids) of the US Environmental Protection Agency, OPPTS Public Drafted, April 1996.
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light / 8 hours dark
- Light intensity: Approximately 400 lux
EFFECT PARAMETERS MEASURED : Daphnia were considered to be immobile if they were unable to swim within approximately 15 seconds following gentle agitation of the test vessel. The numbers of mobile, immobile and floating Daphnia were counted approximately 24 and 48 hours after the start of the study.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Range finding study
- Test concentrations: First range-finding study = 10, 100, and 1000 mg/L; second range-finding study = 0.01, 0.1, 1, 10, and 100 mg/L.
- Results used to determine the conditions for the definitive study: Yes - Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 11.1 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 6.4 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 35.8 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% Confidence limits = 33.1 - 42.9 mg/L
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 21.9 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Reported statistics and error estimates:
- Note that the original study report indicated effect levels (EC50 and NOEC) in terms of the initial measured concentrations. Effect concentrations were recalculated as the geometric mean of initial and 48-hour (expired) concentrations.
- Validity criteria fulfilled:
- yes
- Conclusions:
- In terms of geometric mean concentrations (fresh and 48-hour expired media), the 48-hour EC50 of 2-bromo-3,3,3- trifluoropropene for the immobilisation of Daphnia magna was 11.1 mg/L and the ‘no-observed effect concentration’ was 6.4 mg/L.
Reference
Description of key information
EC50, 48 hours, Daphnia Magna = 11.1 mg/L
NOEC, 48 hours, Daphnia Magna = 6.4 mg/L
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- 11.1 mg/L
Additional information
An acute toxicity test in Daphnia Magna was conducted (Huntingdon Life Sciences, 2013) to determine the effect of 2-Bromo-3,3,3-trifluoroporopene on aquatic invertebrates following short-term exposure. The study was conducted according to EC test method C2, OECD method 202, and US EPA OPPTS Method 850.1010; the study was performed in compliance with GLP. The test was performed under static conditions; the test vessels were sealed but included a headspace.
In terms of initial measured concentrations, the 48-hour EC50 of 2-bromo-3,3,3- trifluoropropene for the immobilisation of Daphnia magna was 35.8 mg/L (95% confidence limits of 33.1 and 42.9 mg/L) and the ‘no-observed effect concentration’ was 21.9 mg/L.
The observed effect concentations were recalculated as the geometric mean values of the measured values at the start and end of the exposure period; in terms of mean measured values the EC50 was 11.1 mg/L and the NOEC was 6.4 mg/L.
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