Sodium 4-(4-methyl-3-nitrobenzoylamino)benzenesulphonate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1990

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: well performed OECD study with GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not specified

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Sodium 4-(4-methyl-3-nitrobenzoylamino)benzenesulphonate

EC Number:

281-719-9

EC Name:

Sodium 4-(4-methyl-3-nitrobenzoylamino)benzenesulphonate

Cas Number:

84029-45-8

Molecular formula:

C14H12N2O6S.Na

IUPAC Name:

sodium 4-(4-methyl-3-nitrobenzoylamino)benzenesulphonate

Details on test material:

purity: 89,9 %

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

after addition of the substance the pH raised to 9.1. Therefore the solution was neutralized with 4.5 ml 3.7 % HCl.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebrabärbling
- Strain: Hamilton-Buchanan
- Source: West Aquarium, 3422 Bad Lauterberg
- Age at study initiation : about three months
- Length at study initiation: 2.8 - 3.4 cm
- Feeding during test: no

ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions: same as test
- Type and amount of food: Tetra Standard Mix, Tetra Werke, Melle
- Feeding frequency: twice a day ad libitum

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

no

Test conditions

Test temperature:

21.4 - 22.0 °C

pH:

7.5 - 8.1

Dissolved oxygen:

6.0 - 8.8 mg O2 /L

Nominal and measured concentrations:

nominal: 500 mg/L
measured: 501 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: open glass vessel (30 cm x 22 cm x 24 cm) calibrated to 10 liters
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to ISO/DIS 7346/1

OTHER TEST CONDITIONS
- Adjustment of pH: at preparation of the stock solution
- Photoperiod: 12 hours
- Light intensity: 700 Lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

TEST CONCENTRATIONS. 0 m/L and 500 mg/L

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

The fish in the control group showed impact on the swimming behaviour as well as on appearance an behaviour. The fish of the control group showed additionally impact on breathing

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Based on the test result the LC 50 is greater than 500 mg/L and the the test substance has therefore not to be classifed for acute toxicity to fish.

Executive summary:

The substance was tested for acute toxicity to fish according to OECD guideline 203. One concentration of 500 mg/L was used and a control with 10 Zebrabärbling each. No mortality occured aftre 96 hours, but the fish of both groups showed some abnormal behaviour