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EC number: 937-688-5 | CAS number: 1391530-05-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2009
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study; well documented study report
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 835.3110 (Ready Biodegradability)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- N,N'-bis[3-(dimethylamino)propyl]-9-nonyl-10-octylnonadecanediamide; N-[3-(dimethylamino)propyl]-9-[2-(7-{[3-(dimethylamino)propyl]carbamoyl}heptyl)-3-[(2E)-oct-2-en-1-yl]-4-pentylcyclohexyl]nonanamide; N-[3-(dimethylamino)propyl]-9-[2-(7-{[3-(dimethylamino)propyl]carbamoyl}heptyl)-3-octyl-4-pentylcyclohexyl]nonanamide
- EC Number:
- 937-688-5
- Cas Number:
- 1391530-05-4
- Molecular formula:
- C46H82N4O2 to C46H94N4O2
- IUPAC Name:
- N,N'-bis[3-(dimethylamino)propyl]-9-nonyl-10-octylnonadecanediamide; N-[3-(dimethylamino)propyl]-9-[2-(7-{[3-(dimethylamino)propyl]carbamoyl}heptyl)-3-[(2E)-oct-2-en-1-yl]-4-pentylcyclohexyl]nonanamide; N-[3-(dimethylamino)propyl]-9-[2-(7-{[3-(dimethylamino)propyl]carbamoyl}heptyl)-3-octyl-4-pentylcyclohexyl]nonanamide
- Details on test material:
- - Physical state: amber coloured viscous liquid
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- Source:
A mixed population of activated sewage sludge micro-organisms was obtained from the aeration stage of the Severn Trent Water Plc sewage treatment plant at Loughborough, Leicestershire UK, which treats predominantly domestic sewage.
Culture medium: The culture medium used in this study was that recommended in the OECD guidelines.
Preparation:
The activated sewage sludge sample was washed three times by settlement and resuspension in culture medium to remove any excessive amounts of dissolved organic carbon that may have been present. The washed sample was then maintained on continuous aeration in the laboratory at a temperature of 21 degrees C and used on the day of collection. Determination of the suspended solids level of the activated sewage sludge was carried out by filtering a sample of the washed activated sewage sludge by suction through pre-weighed GF/A filter paper using a Buchner funnel. Filtration was then continued for a further 3 minutes after sensing the filter three successive times with 10 ml of deionised reverse osmosis water. The filter paper was then dried in an oven at approximately 105 degrees C for at least 1 hour and allowed to cool before weighing. This process was repeated until a constant weight was attained.
Concentration:
The suspended solids concentration was equal to 3.3 g/l prior to use. - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 10 other: mg/L carbon
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- Pre-Study Solubility Work
Pre-study solubility work was conducted by ultrasonication and high shear mixing. This work confirmed that the test material was poorly soluble. Additional solubility work was conducted to ascertain the method that would give the best testable dispersion. This work concluded that it was appropriate to dissolve the test material in solvent prior to coating the silica gel with the solvent test material stock solution and then removing the solvent as this method appeared to increase the dispersibility of the test material over the other methods investigated.
TEST SYSTEM
-The following test preparations were prepared and inoculated in 5 litre glass culture vessels each containing 3 litres of solution:
a) A control, in duplicate, consisting of inoculated culture medium plus silic gel
b) The standard material (sodium benzoate) in duplicate in inoculated culture medium plus silic gel to give a final concentration of 10 mg carbon/l.
c) The test material dissolved in acetone coated on silica gel in duplicate in inoculated culture medium to give a final concentration of 10 mg carbon/l.
d) The test material dissolved in acetone coated on silic gel plus the standard material in inoculated culture medium to give a final concentration of 20 mg carbon/l to act as a toxicity control (one vessel only).
-Silica gel was added to the control and standard material vessels in order to maintain consistency between these vessels and the test material vessels. Each test vessel was inoculated with the prepared inoculum at a final concentration of 30 mg suspended solids per liter. The test was carried out in a temperature controlled room at 21 degrees C in darkness.
-Approximately 24 hours prior to addition of the test and standard materials the vessels were filled with culture medium and inoculum and aerated overnight. On Day 0 the test and standard materials were added and the volume in all the vessels adjusted to 3 litres by the addition of culture medium The culture vessels were sealed and CO2 free air bubbled through the solution at a rate of approximately 40 ml/minute and stirred continuously by magnetic stirrer. The CO2 free air was produced by passing compressed air through a glass column containing self indicating soda lime granules.
-The CO2 produced by degradation was collected in Dreschel bottles containing sodium hydroxide. The CO2 absorbing solutions were prepared using purified degassed water.
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 17
- Sampling time:
- 28 d
- Details on results:
- The test material attained 17% degradation after 28 days. The toxicity control attained 46% degradation after 14 days. Sodium benzoate attained 109% degradation after 14 days and 93% degradation after 28 days. Observations made on Day 0 of the test period showed the contents of the control vessels to be light brown dispersions and the contents of the standard material vessels were light brown dispersions with no undissolved standard material visible. The test material vessels were observed to be light brown dispersions with no undissolved test material visible. The toxicity control vessel contained a light brown dispersion with no undissolved test or standard material visible.
BOD5 / COD results
- Results with reference substance:
- Sodium benzoate attained 109% degradation after 14 days and 93% degradation after 28 days
Any other information on results incl. tables
Percentage Biodegradation Values
Day |
% Degradation Sodium Benzoate |
% Degradation Test Material |
% Degradation Test Material plus Sodium Benzoate; Toxicity Control |
0 |
0 |
0 |
0 |
2 |
25 |
0 |
19 |
6 |
74 |
17 |
56 |
8 |
64 |
18 |
55 |
10 |
103 |
11 |
44 |
14 |
109 |
15 |
46 |
21 |
100 |
23 |
NA |
28 |
91 |
26 |
NA |
29 |
93 |
17 |
NA |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: Not Readily Biodegradable under Test Conditions
- Conclusions:
- The test material attained 17% degradation after 28 days. The reference substance attained 93% degradation after 28 days
- Executive summary:
The objective of the study was to measure the amount of carbon dioxide produced from the biodegradation of the test substance under the conditions tested. The study was conducted according to procedures specified by the OECD Guideline for Testing of Chemicals, Method 301B, EEC Method C.4-C, and US EPA Guideline OPPTS 835.3110.
In view of the difficulties associated with the evaluation of the biodegradability of organic compounds with low water solubility a modification to the standard method of preparation of the test concentration was performed. An approach endorsed by the International Standards Organisation and the published literature is to dissolve the test material in an auxiliary solvent prior to adsorption onto granular silica gel. Using this method the test material is evenly distributed throughout the test medium and the surface area of the test material exposed to the test organisms is expected to increase.
The test material at a concentration of 10 mg carbon per liter was exposed to activated sewage sludge microbes with culture medium in sealed culture vessels in the dark at approximately 21 degrees C for 28 days.
The degradation of the test material was assessed by the determination of carbon dioxide produced. Control solutions with inoculum and the standard material, sodium benzoate, together with a toxicity control were used for validation purposes.
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