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EC number: 223-989-2 | CAS number: 4156-21-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
At the limit concentration of 143 mg/L of the test item Active Substance of Granofin Easy F-90 liq in Aqueous Solution, no biologically or statistically significant effects were determined. The No Observed Effect Concentration (NOEC) and the Lowest Observed Effect Concentration (LOEC) were assessed as follows:
NOEC : 143 mg/L
LOEC : > 143 mg/L
All provided effect values are based on the nominal concentration of the test item Active Substance of Granofin Easy F-90 liq in Aqueous Solution.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 143 mg/L
Additional information
TheDaphnia magnaReproduction
Test (Semi-Static, 21 d) of Active Substance of Granofin Easy F-90 liq
in Aqueous Solution was conducted as a limit test with the
concentration of 143 mg/L of the test item, which corresponds to 100
mg/L of the main component, according to OECD 211 (2008) from 2012-05-30
to 2012-06-21, with the definitive
exposure phase from2012-05-30 to 2012-06-20atDr.U.Noack-Laboratorien,Käthe-Paulus-Str.
1, 31157 Sarstedt, Germany.
The selected test organism wasDaphnia magnaSTRAUS (Clone 5). Ten
daphnids individually held were used for the limit concentration and the
control. At the test start the daphnids were 2 to 24 h old. The test
method was semi-static with a renewal of the test solutions three times
per week. The aim of the Daphnia Reproduction Test over 21 days was to
assess effects on the reproduction capacity and other test item-related
effects, including occurrence of aborted eggs and stillborn juveniles,
time of production of first brood, adult mortality, intrinsic rate of
natural increase, dry body weight and length of the parent daphnids.
The tested limit concentration of the test item Active Substance of
Granofin Easy F-90 liq in Aqueous Solution and the control were
analytically verified by HPLC-DAD of samples on days 0, 7, 16 (fresh
media, 0 hours) and on days 2, 9, 19 (old media, 48 and 72 hours).
Details of the analytical method are presented in part 11.
The measured concentrations of the test item Active Substance of Granofin Easy F-90 liq in Aqueous Solution were in the range of 94 to 101 % of the nominal values in samples of the fresh media (0 hours), and 87 to 93 % of the nominal values in the old media (48 and 72 hours) of the limit concentration and the control. The results given are based on the nominal concentration of 143 mg/L of the test item Active Substance of Granofin Easy F-90 liq in Aqueous Solution.
· The averagevalues ofjuveniles per parental daphnidwere 123 for the control and 121 for the limit concentration of 143 mg/L after 21 days. The reproductive output of daphnids in the limit concentration was comparable to the reproductive output of daphnids in the control(t-test,p = 0.05).
· The coefficient of variation of thenumber of living offspringproduced per parent was 10 % for the control and 7 % for thelimit concentration
· Theintrinsic rates of natural increase (IR)of the surviving parent daphnids accounting for generation time and number of offspring were calculated as a measure for population growth and maintenance. The IR of thelimit concentrationwas comparabletothe control group (t-test,p = 0.05).
· Noadult mortalityor immobilization of parental daphnids was observedin the control andthelimit concentration.
· Nodead juveniles and aborted eggswere observed in the control and thelimit concentration.
Five broods were observed during the test period for all parental daphnids of the limit concentration and the control. Thefirst broodwas released from all parent daphnids in all treatments between day 8 and 9.
· The
mean drybody weight and total body lengthsof
all parental daphnids of the test and the control groups were determined
at the end of the study.
Themeandry body weightof the parental daphnids was 1.06 mg per
daphnid in the control and 0.77 mg per daphnid in the limit
concentration.
Themean bodylengthof
the parental daphnids was 5.20 mm per daphnid in the control and 5.00 mm
per daphnid in the limit concentration.
· Nomalesandephippia(winter eggs) were observed in the respective control or test groups.
A summary of all test item related effects is given inTable 1. No biologically or statistically significant effect could be determined in the limit concentration of 143 mg/Lcompared to the control.
Table1: Summary of all Test Item Related Effects
(Based on the nominal concentration of the test itemof 143 mg/L)
Active Substance of Granofin Easy F-90 liq in Aqueous Solution |
|||
Effects |
Control |
Nominal Concentration 143 mg/L |
Significance |
Mean Number of Juveniles |
123 ± 12 |
121 ± 8 |
no1) |
Coefficient of Variation of theMean Number of Juveniles per Producing Parent |
10 |
7 |
no |
Intrinsic Rate of Natural Increase (Mean±SD) |
0.48 ± 0.03 |
0.49 ± 0.01 |
no1) |
Appearance of First Brood (Mean Day±SD) |
8.1 ± 0.3 |
8.0 ± 0.0 |
— |
Number of Broods |
5 |
5 |
— |
Stillborn Juveniles and Aborted Eggs |
0 |
0 |
— |
Adult Mortality after 21 Days [%] |
0 |
0 |
no |
Parent Daphnids: |
1.06 |
0.77 |
— |
Parent Daphnids: |
5.20 ± 0.26 |
5.00 ± 0.17 |
no |
NOEC |
143 mg/L |
||
LOEC |
> 143 mg/L |
Thewater quality parameters(pH-value, dissolved oxygen, water hardness and temperature) were within the acceptable limits.
· To assure thevalidity of the test systemand the test conditions at the test facility, an acute immobilization test according to DIN 38412 L 11 is carried out withpotassium dichromateas reference item once per month. This reference test is required by the test guideline OECD 211 (2008).
The EC50of the reference item of1.74 mg/L (95 % confidence limits: 1.49 - 2.00 mg/L)after 24 h is within the prescribed concentration range of 1.0 - 2.5 mg/L of quality criteria according toAQS P 9/2 (05/1996) for daphnids clone 5 cultured in Elendt M4 medium. The EC50-value of the reference item is also within the recommended range of 0.6 - 2.1 mg/L according to OECD-Guideline 202.
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