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EC number: 240-464-3 | CAS number: 16415-12-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1989-12-12 to 1989-12-16
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: 1.5 g of test substance was added to 1.5 L of dilution water and stirred vigorously for 24 hours on a magnetic stirrer at a temperature of 25+/1ºC. The aqueous phase was then decanted into the test vessel as the test medium.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): It is likely that the solubility of the substance in the test medium was exceeded. An oil-like substance was observed on the surface of the test media. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebrafish
- Source: obtained from a commercial hatchery; M.B. Ruysbroek B.V., Maassluis, The Netherlands.
- Length at study initiation (length definition, mean, range and SD): 2.2+/-0.1 cm
- Weight at study initiation (mean and range, SD): 0.08+/-0.01 g - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 215 mg/l as CaCO3
- Test temperature:
- 25+/-1ºC
- pH:
- 7.6-8.0
- Dissolved oxygen:
- 6.1-8.5 mg/L
- Salinity:
- not applicable
- Nominal and measured concentrations:
- Nominal loading rates: 0(Control) and 1000 mg/L.
It is likely that the solubility of the substance in the test medium was exceeded. An oil-like substance was observed on the surface of the test media. - Details on test conditions:
- TEST SYSTEM
- Test vessel: beaker
- Type (delete if not applicable): open
- Material, size, fill volume: glass, 2 L with 1.5 L fill volume
- Aeration: none
- Renewal rate of test solution (frequency/flow rate): daily
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.52 g/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Synthetic freshwater (DSWL) prepared by the additional of salts to groundwater from near Linschoten, The Netherlands.
- Culture medium different from test medium: no
- Intervals of water quality measurement: Daily
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light, 8 h dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mortality after 24, 48, 72 and 96 h. - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LL50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOELR
- Effect conc.:
- >= 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: 0
- Mortality of control: 0
- Other adverse effects control: 0
- Abnormal responses: 0
- Effect concentrations exceeding solubility of substance in test medium: It is likely that the solubility of the substance in the test medium was exceeded. An oil-like substance was observed on the surface of the test media. - Reported statistics and error estimates:
- No toxic effects were observed in the test and therefore statistical analysis of the results was not required.
- Sublethal observations / clinical signs:
No toxic effects were observed in the test.
- Validity criteria fulfilled:
- yes
- Conclusions:
- A 96-h LL50 value of >1000 mg/L and NOELR of ≥1000 mg/L have been determined for the effects of the test substance on mortality of Brachydanio rerio based on nominal loading rate of the test substance.
Reference
Description of key information
LL50 (96 h) > 1000 mg/L (nominal, OECD 203, Danio rerio)
Key value for chemical safety assessment
Additional information
One limit test is available with the substance hexadecyl(trimethoxy)silane (CAS No. 16415-12-6) testing the short term toxicity towards fish according to a standard guideline (OECD 203) and GLP. The study was conducted under semi-static conditions and Danio rerio was used as the test organism. An LL50 (96 h) value of >1000 mg/L and a NOELR (96 h) value of ≥ 1000 mg/L revealing that no short term effects arise from the substance towards fish.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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