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EC number: 203-040-9 | CAS number: 102-56-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1989
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study performed to OECD 203 including GLP certificate and well documented
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- other: "Letale Wirkung beim Zebrabärbling Brachydanio rerio" (LC0, LC50, LC100; 48 - 96 Stunden) - recommendation of the GErman Federal Environmetal Agency, Texte 16/84, Umweltbundesamt
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- not specified
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- not specified
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2,5-dimethoxyaniline
- EC Number:
- 203-040-9
- EC Name:
- 2,5-dimethoxyaniline
- Cas Number:
- 102-56-7
- Molecular formula:
- C8H11NO2
- IUPAC Name:
- 2,5-dimethoxyaniline
- Details on test material:
- molecular formula C8-H11-N-O2
molecular weight 153,5 g/mol
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Actual substance concentration were determined at the beginning, after 48 h and at the end of the experiment at 96 h. Therefore 100 ml of the solution was taken out of the bath middle. The analytical data were given above 80 % of target concentration.
Target (mg/l) meassured median (mg/l) measured (%)
100 100.1 100.1
220 220.0 100.0
500 562.0* 112,4
* median after 0 and 48 hours
Test solutions
- Vehicle:
- yes
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- Strain: Hamilton-Buchanan
Origin West Aquarium, 3422 Bad Lauterberg, Germany
Date of hatching: 1989-01-12 / 1989-01-19
Date of transfer to test basins: 1989-04-12 / 1989-04-19
Acclimation period of 14 days in dilution water (test water according to ISO/DIS 7346/1):
Temperature 22 +/- 1 °C
O2-content >= 80 % of Saturation
Illumination 12 h daily
Biomasse loading: <= 1 g Fish / l water
Feeding: 2 times a day ad libitum
Food: Tetra Min, Tetra Werke, Melle, Germany
The experiment was perfomred in three parts: part 1 0, 0* and 100 mg/lwith 0* being the vehicle control with Tween 80), part 2 (0 and 500 mg/l), part 3 (0 and 220 mg/l). From the fish for each test-part 10 have been choosen for determination of body length. Those enimals were not used for the test
part 1: n=10, range = 2.6 - 3.3 cm, mean = 2,9 cm, SD = 0,20 cm
part 2: n=10, range = 2.8 - 3.5 cm, mean = 3.1 cm, SD = 0,26 cm
part 1: n=10, range = 2.9 - 3.4 cm, mean = 3.1 cm, SD = 0,19 cm
total: n=30, range = 2.6 - 3.5 cm, mean = 3.1 cm, SD = 0,24 cm
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- none
Test conditions
- Hardness:
- test water (reconstituted fresh water) according to ISO/DIS 7346/1
- Test temperature:
- 22 +/- 1 °C
- pH:
- concentration groups 7.3 - 8.1
controls 7.5 - 8.2 - Dissolved oxygen:
- concentration groups 4.6 - 8.6 mg/l
controls 4.2 - 9.1 mg/l - Salinity:
- test water (reconstituted fresh water) according to ISO/DIS 7346/1
- Nominal and measured concentrations:
- At the start, after 48 h and at the end of the test (96 h) the actual concentration for all the dilutions were determined (100 ml samples each). The arithmetic means fo the determined concentrations were (mg/l):
nominal analytically measured
100 100,1
220 220,0
500 562,0 - Details on test conditions:
- Test system:
Test vessel: Glass basins, volume 10 l (30 cm * 22 cm * 24 cm)
Aeration: For concentration group 220 mg/l of part 3 and the Tween group from 72 h after start of the experiment aeratio had been applied to acertain a minimum O2-saturation from 60 % saturation. For all other groups saturation was not neccessary.
No of organism per vessel: 10
No of vessels per concentration: one
No of vessels per control (replicas): three
No of vessels per vehicle control: one
Photoperiod: 12 h, approx 700 LUX
Adjustment of pH: no - Reference substance (positive control):
- yes
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- LC0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 220 - < 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 220 - < 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC100
- Effect conc.:
- 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In an acute toxicity test on Brachydanio rerio equivalent to OECD 203, the substance Aminohydrochinondimethylether was tested for acute toxicity. The LC0 was 100 mg/l after both, 48 and 96 hours. The LC50 was determined to be between 220 and 500 mg/l, after 48 and 96 hours.
- Executive summary:
In an akute toxicity test with Brachydanio rerio equivalent to OECD 203, the substance Aminohydrochinondimethylether was tested for acute toxicity. The LC0 was 100 mg/l after both, 48 and 96 hours. The LC50 was determined to be between 220 and 500 mg/l, after 48 and 96 hours.
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