Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 692-759-5 | CAS number: 1040916-84-4
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin corrosion: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- other: Summary Report
- Title:
- Unnamed
- Year:
- 2 011
- Report date:
- 2011
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 431 (In Vitro Skin Corrosion: Human Skin Model Test)
- GLP compliance:
- no
- Remarks:
- non-GLP, but in accordance with the guideline
Test material
- Reference substance name:
- 1,3-diethyl-1H-imidazol-3-ium acetate
- EC Number:
- 692-759-5
- Cas Number:
- 1040916-84-4
- Molecular formula:
- C7 H13 N2 . C2 H3 O2
- IUPAC Name:
- 1,3-diethyl-1H-imidazol-3-ium acetate
- Details on test material:
- Batch: 10362/11-25-5
pH value: ca. 7 (undiluted test substance)
Constituent 1
Test animals
- Species:
- other: human reconstituted epidermis model
Test system
- Vehicle:
- unchanged (no vehicle)
- Duration of treatment / exposure:
- 3 min and 1 hour
- Number of animals:
- 2 samples per application time
- Details on study design:
- The test consists of a topical exposure of the test substance to the surface of a human reconstituted epidermis model for 3 minutes and 1 hour followed by a cell viability test. Cell viability is measured by dehydrogenase conversion of the yellow, water-soluble MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide), present in cell mitochondria, into a blue formazan salt that is measured quantitatively after isopropanol – extraction from the tissues. The optical density of the extracts of test substance treated tissues is compared to negative control values from tissue treated with highly de-ionized water and is expressed as relative tissue viability.
Results and discussion
In vitro
Resultsopen allclose all
- Irritation / corrosion parameter:
- % tissue viability
- Value:
- 105
- Remarks on result:
- other: 3 min (mean)
- Irritation / corrosion parameter:
- % tissue viability
- Value:
- 107
- Remarks on result:
- other: 1 h (mean)
Any other information on results incl. tables
Mean tissue viability (% of negative control) | Prediction |
| 3 min:<50 | corrosive |
| 3 min: >= 50 and 1 hour: < 15 | corrosive |
| 3 min: >= 50 and 1 hour: >= 15 | non-corrosive |
Non-corrosive
Valid positive control with mean viability of 26 % after 3 min exposure and 7 % after 1 hour exposure, compared to negative control.
Applicant's summary and conclusion
- Interpretation of results:
- other: non corrosive
- Conclusions:
- Based on the observed results it was concluded, that EEIM Acetat does not show a corrosive potential in the EpiDerm™ skin corrosion test under the test conditions chosen.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
