Phenol, mono- + distyrenated (MSP+DSP)

Endpoint summary

Additional information

Short-term toxicity to fish

For assessment of the short-term toxicity of phenyl, styrenated to fish, two valid studies both according to OECD TG 203 are available (Aniol/STZ 2010 and CHRIP/NITE 1997). In the study of Aniol/STZ 2010, water-accommodated fractions (WAF) were used. Results are reported as loading rates. These were converted to measured concentrations (arithm. mean) using the analytical monitoring results of the test.

The 96h LC50 values of both studies fall within the same range. The lower value of 1.77 mg/L (Aniol/STZ 2010) is used in the further assessment of phenol, mono- & distyrenated (Novares LS 500).

 

Long-term toxicity to fish

In a study according to OECD TG 204 with Japanese ricefish, a 14d NOEC of 1.9 mg/L and a 14d LC50 of 3.8 mg/L were determined (CHRIP/NITE 1997). A recent chronic toxicity study in fish with the test substance 4-(1-phenylethyl)phenol (4-MSP) according to OECD TG 234 (Fish Sexual Development Test) including early and juvenile life stages as well as sexual differentiation failed to show adverse effects on hatching success and rates, larval survival, and juvenile growth at any test concentration until 63 days post-fertilisation. The highest concentration tested (0.188 mg/L) has been adopted as chronic NOEC for the test substance 4-MSP. For the chemical safety assessment of the target substance phenol, mono- & distyrenated, the more conservative NOEC from a long-term toxicity test with daphnia is used (see below, NOEC = 0.115 mg).

 

Short-term toxicity to aquatic invertebrates

For assessment of the short-term toxicity of phenol, mono- &distyrenated to aquatic invertebrates, two valid studies both according to OECD TG 202 are available (Heyner/BiLaCon 2000 and CHRIP/NITE 1997). In the study of Heyner/BiLaCon 2000, water-accommodated fractions were used. Results are reported as loading rates. These were converted to measured concentrations (arithm. mean) using the analytical monitoring results of the test.

The 48h EC50 values of both studies fall within the same range. The value of 4.6 mg/L of the CHRIP/NITE 1997 study is used in the further assessment of phenol, mono- & distyrenated (Novares LS 500).

 

Long-term toxicity to aquatic invertebrates

For assessment of the long-term toxicity of phenol, mono- & distyrenated to aquatic invertebrates, two valid studies are available (Noack/NOACK 2006 and CHRIP/NITE 1997). In the study of Noack/NOACK 2006, distyrenated phenol was used as test substance. Distyrenated phenol will be acceptable as supporting substance for phenol, mono- & distyrenated as Novares LS 500 contains ca. 20 to 40% of this substance.

The 21d NOEC values of both studies fall within the same range. The value of 0.115 mg/L determined with the supporting substance distyrenated phenol (Noack/NOACK 2006) is slightly lower. As conservative approach, it is used in the further assessment of phenol, mono- & distyrenated (Novares LS 500).

 

Toxicity to aquatic algae and cyanobacteria

For assessment of the toxicity of phenol, mono- & distyrenated to aquatic algae and cyanobacteria, two valid studies both according to OECD TG 201 are available (Aniol/STZ 2010 and CHRIP/NITE 1997). In the study of Aniol/STZ 2010, water-accommodated fractions were used. Results are reported as loading rates. These were converted to measured concentrations (arithm. mean) using the analytical monitoring results of the test.

Results of the Aniol/STZ 2010 study are lower by a factor of about 5 to 8. These values (72h EC50: 1.35 mg/L; 72h NOEC: 0.42 mg/L) are used in the further assessment of phenol, mono- & distyrenated (Novares LS 500)

 

Toxicity to microorganisms

For the assessment of the toxicity to microorganisms, one valid study is available (Aniol/STZ 2010). In this 3h respiratory inhibition study (limit test with a nominal concentration of 100 mg/L), no inhibition of the respiratory rate was observed (NOEL = 100 mg/L; EL50 > 100 mg/L).

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Note: The CHRIP/NITE 1997 studies were performed for the Japanese National Institute of Technology and Evaluation (NITE)/Ministry of the Environment (MoE) and are reported in databases (CHRIP, J-CHECK) of NITE. Test substance was phenol, styrenated (CAS Nr 61788-44-1). Composition of the test substance is not specified. As the name of the test substance under the Japanese Chemical Substance Control Law is (mono (or di or tri)-(alpha-methylbenzyl)phenol), it is concluded that the test substance is a mixture of mono-, di-, and tristyrenated phenol. Similarity with Novares LS 500 is considered to be adequate to use data from these tests as substantial/supporting evidence for the characterisation of Novares LS 500.