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EC number: 482-140-6 | CAS number: 13641-96-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to terrestrial plants
Administrative data
- Endpoint:
- toxicity to terrestrial plants: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 01 Mar 2011 - 25 Mar 2011
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- Because no significant phytotoxicity was showed in the range-finding test (p<0.05), no definitive testing was carried out.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
- Report date:
- 2011
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.4200 (Seed Germination/Root Elongation Toxicity Test)
- Version / remarks:
- 1996
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- -
- EC Number:
- 482-140-6
- EC Name:
- -
- Cas Number:
- 13641-96-8
- Molecular formula:
- Hill formula: C6 H7 N O3 CAS formula: C6 H7 N O3
- IUPAC Name:
- 2-isocyanatoethyl prop-2-enoate
- Test material form:
- liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test substrate
- Vehicle:
- no
- Details on preparation and application of test substrate:
- - Method of mixing into soil (if used): Fresh test solutions were added to glass bottles that have been filled with 100 g clean quartz sand.
- The test solution of AOI was prepared by directly adding appropriate amount of the test item into distilled water.
Test organisms
open allclose all
- Species:
- Zea mays
- Plant group:
- Monocotyledonae (monocots)
- Details on test organisms:
- - Common name: Corn
- Source of seed: Nanjing Research lnstitute of Vegetables and Flowers
- Prior seed treatment/sterilization: Only untreated seed (not treated with fungicides, repellents, etc.)
- Seed storage: coId storage (5 °C) in moisture-proof containers; seed moisture < 10%
- Species:
- Daucus carota
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: Carrot
- Source of seed: Nanjing Research lnstitute of Vegetables and Flowers
- Prior seed treatment/sterilization: Only untreated seed (not treated with fungicides, repellents, etc.)
- Seed storage: coId storage (5 °C) in moisture-proof containers; seed moisture < 10%
- Species:
- other: Sesamum indicum
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: Sesame
- Source of seed: Nanjing Research lnstitute of Vegetables and Flowers
- Prior seed treatment/sterilization: Only untreated seed (not treated with fungicides, repellents, etc.)
- Seed storage: coId storage (5 °C) in moisture-proof containers; seed moisture < 10%
- Species:
- other: Vigna radiate
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: Mung bean
- Source of seed: Nanjing Research lnstitute of Vegetables and Flowers
- Prior seed treatment/sterilization: Only untreated seed (not treated with fungicides, repellents, etc.)
- Seed storage: coId storage (5 °C) in moisture-proof containers; seed moisture < 10%
- Species:
- Triticum aestivum
- Plant group:
- Monocotyledonae (monocots)
- Details on test organisms:
- - Common name: Common wheat
- Source of seed: Nanjing Research lnstitute of Vegetables and Flowers
- Prior seed treatment/sterilization: Only untreated seed (not treated with fungicides, repellents, etc.)
- Seed storage: coId storage (5 °C) in moisture-proof containers; seed moisture < 10%
- Species:
- Brassica oleracea var. capitata
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: Cabbage
- Source of seed: Nanjing Research lnstitute of Vegetables and Flowers
- Prior seed treatment/sterilization: Only untreated seed (not treated with fungicides, repellents, etc.)
- Seed storage: coId storage (5 °C) in moisture-proof containers; seed moisture < 10%
- Species:
- Glycine max (G. soja)
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: Soybean
- Source of seed: Nanjing Research lnstitute of Vegetables and Flowers
- Prior seed treatment/sterilization: Only untreated seed (not treated with fungicides, repellents, etc.)
- Seed storage: coId storage (5 °C) in moisture-proof containers; seed moisture < 10%
- Species:
- Lactuca sativa
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: Lettuce
- Source of seed: Nanjing Research lnstitute of Vegetables and Flowers
- Prior seed treatment/sterilization: Only untreated seed (not treated with fungicides, repellents, etc.)
- Seed storage: coId storage (5 °C) in moisture-proof containers; seed moisture < 10%
- Species:
- Cucumis sativus
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: Cucumber
- Source of seed: Nanjing Research lnstitute of Vegetables and Flowers
- Prior seed treatment/sterilization: Only untreated seed (not treated with fungicides, repellents, etc.)
- Seed storage: coId storage (5 °C) in moisture-proof containers; seed moisture < 10%
- Species:
- Lycopersicon esculentum
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: Tomato
- Source of seed: Nanjing Research lnstitute of Vegetables and Flowers
- Prior seed treatment/sterilization: Only untreated seed (not treated with fungicides, repellents, etc.)
- Seed storage: coId storage (5 °C) in moisture-proof containers; seed moisture < 10%
Study design
- Test type:
- seed germination/root elongation toxicity test
- Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- no
- Total exposure duration:
- 7 d
Test conditions
- Test temperature:
- 24 - 25 °C
- pH:
- 7.4 - 7.8
- Details on test conditions:
- TEST SYSTEM
- Test container (type, material, size): Glass bottles
- Amount of soil: 100 g quartz sand
- Method of seeding: The seed was positioned on the substrate allowing adequate room for anticipated growth.·The radicle end of the seed was aligned in the direction of this growth.
- No. of seeds per container: 15
- No. of replicates per treatment group: 1
- No. of replicates per control: 1
GROWTH CONDITIONS
- Photoperiod: complete darkness
- Day/night temperatures: 25 ± 1 °C
- Relative humidity (%): 70 ± 5%
EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
For each test species the number of seeds that germinate was counted and root lengths were measured after 7 days.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10
- Range finding study
- Test concentrations: 10, 100 and 1000 mg/L
- Results used to determine the conditions for the definitive study: Because no significant phytotoxicity was showed in the range-finding test (p<0.05), no definitive testing was carried out. - Nominal and measured concentrations:
- Nominal: 0, 10, 100, 1000 mg/L
- Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Species:
- Lycopersicon esculentum
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 other: mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: germination and root length
- Species:
- Cucumis sativus
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 other: mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: germination and root length
- Species:
- Lactuca sativa
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 other: mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: germination and root length
- Species:
- Glycine max (G. soja)
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 other: mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: germination and root length
- Species:
- Brassica oleracea var. capitata
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 other: mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: germination and root length
- Species:
- Triticum aestivum
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 other: mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: germination and root length
- Species:
- other: Vigna radiate
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 other: mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: germination and root length
- Species:
- other: Sesamum indicum
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 other: mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: germination and root length
- Species:
- Daucus carota
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 other: mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: germination and root length
- Species:
- Zea mays
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 other: mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: germination and root length
- Details on results:
- SEED GERMINATION
- Percent seed germination: No relation between seeds germination and the test item at different concentrations was observed.
- Root length: Root lengthes between control and treatment groups showed no significant difference at the 0.05 level.
- Other effects: At the end of the test, more than 65% of the control seeds germinated and the roots developed were longer than 20 mm. - Reported statistics and error estimates:
- The mean root length for each concentration were compared with the control mean using an multiple comparison method of Dunnett´s tests (ANOVA, SPSS 17.0).
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
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