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EC number: 610-472-5 | CAS number: 49707-23-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2009-10-28 to 2010-01-28
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Monitored GLP study, following international guidelines
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- N-(2,2-dimethoxyethyl)prop-2-enamide
- EC Number:
- 610-472-5
- Cas Number:
- 49707-23-5
- Molecular formula:
- C7 H13 N1 O3
- IUPAC Name:
- N-(2,2-dimethoxyethyl)prop-2-enamide
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): supplied by the sewage works of Rossdorf, Germany
- Storage conditions: after inital washing aeration overnight
- Storage length: overnight
- Preparation of inoculum for exposure:dilution to stock concentration in test water
- Pretreatment: 3x wash in tap water, centrifugation and resuspension, finally in test water
- Concentration of sludge: stock suspension: 1.5 g/L (on dry matter base)
- Initial cell/biomass concentration: final concentration in test vessel: 31 mg/L
- Test water: reconstituted by adding analytical grade salt solutions - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 25 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: see block "Any other informations on matgerial and methods, incl. tables"
- Additional substrate: no
- Solubilising agent (type and concentration if used): not applicable
- Test temperature: 22 +-1 °C
- pH: Start of test: 7.5; end of test: 6.7 - 7.6
- Aeration of dilution water: yes (before dilution)
- Suspended solids concentration: final concentration in test vessel: 31 mg/L
- Continuous darkness: yes
TEST SYSTEM
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: no data
- Method used to create anaerobic conditions: not applicable
- Measuring equipment: BSB Sensomat system, Aqualytic Dortmund
- Test performed in closed vessels due to significant volatility of test substance: no
- Test performed in open system: no
- Details of trap for CO2 and volatile organics if used: Potassium hydroxide solution (45%)
SAMPLING
- Sampling frequency: daily
- Sampling method: not applicable
- Sterility check if applicable: not applicable
- Sample storage before analysis: not applicable
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 x
- Abiotic sterile control: 1
- Toxicity control:1 x (HgCl2)
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- 60
- Sampling time:
- 28 d
- Details on results:
- Over the whole test period 60% consumtion/degradation could be observed. Results calculated with THOD (NO3) and ThOD (NH4) were identical.
BOD5 / COD results
- Results with reference substance:
- Results with reference substance bencoic acid, sodium salt and the toxic control with HgCl2 were vaild.
Any other information on results incl. tables
1. Cumulative biochemical Oxygen demand over the course of 28 days, raw data, not corrected for Nitrogen
Time |
Flask No. |
|||||||
(days) |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
|
1 |
0 |
0 |
0 |
0 |
50 |
0 |
45 |
|
2 |
0 |
0 |
0 |
0 |
75 |
0 |
70 |
|
3 |
5 |
5 |
5 |
0 |
115 |
0 |
110 |
|
4 |
5 |
5 |
5 |
5 |
120 |
0 |
120 |
|
5 |
5 |
5 |
5 |
5 |
130 |
0 |
125 |
|
6 |
5 |
5 |
5 |
5 |
135 |
0 |
135 |
|
7 |
10 |
5 |
5 |
5 |
140 |
0 |
140 |
|
8 |
10 |
10 |
5 |
10 |
145 |
0 |
145 |
|
9 |
20 |
25 |
10 |
10 |
150 |
0 |
150 |
|
10 |
35 |
40 |
10 |
10 |
150 |
0 |
155 |
|
11 |
45 |
50 |
10 |
10 |
155 |
0 |
165 |
|
12 |
55 |
60 |
10 |
10 |
160 |
0 |
170 |
|
13 |
75 |
80 |
15 |
15 |
165 |
0 |
180 |
|
14 |
100 |
95 |
15 |
15 |
170 |
0 |
210 |
|
15 |
110 |
105 |
15 |
15 |
170 |
0 |
220 |
|
16 |
115 |
110 |
15 |
15 |
175 |
0 |
225 |
|
17 |
120 |
115 |
20 |
15 |
180 |
0 |
230 |
|
18 |
125 |
120 |
20 |
20 |
180 |
0 |
240 |
|
19 |
130 |
125 |
20 |
20 |
185 |
0 |
245 |
|
20 |
130 |
125 |
20 |
20 |
185 |
0 |
250 |
|
21 |
135 |
130 |
25 |
25 |
190 |
0 |
255 |
|
22 |
135 |
130 |
25 |
25 |
190 |
0 |
255 |
|
23 |
135 |
135 |
25 |
25 |
190 |
0 |
255 |
|
24 |
135 |
135 |
25 |
25 |
195 |
0 |
255 |
|
25 |
140 |
135 |
25 |
25 |
195 |
0 |
260 |
|
26 |
140 |
140 |
25 |
25 |
195 |
0 |
260 |
|
27 |
145 |
145 |
25 |
25 |
195 |
0 |
265 |
|
28 |
150 |
150 |
25 |
25 |
195 |
0 |
265 |
|
where:
Flasks 1 and 2: N-(2,2-Dimethoxyethyl)acrylamide (NAAADA)
Flasks 3 and 4: inoculum control
Flask 5: reference(procedure control)
Flask 6: abiotic control
Flask 7: toxicity control
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable, but failing 10-day window
- Conclusions:
- The substance is considered to be readily biodegradable under test conditions of the screening test according to OECD 301F.
- Executive summary:
The test item N-(2,2-Dimethoxyethyl)acrylamide (NAAADA) contains nitrogen, therefore the evaluation of biodegradation has to be based on the assumption that nitrification occurred, in the following expressed as ThODNO3. The criterion for ready biodegradability under the conditions of a manometric respirometry test is the 10-day window, describing the period between reaching at least 10% degradation and 60% degradation. This period should not exceed 10 days. The mean biodegradation of N-(2,2-Dimethoxyethyl)-acrylamide (NAAADA) after 28 days of incubation was 60% (ThODNO3); the 10 day window was reached on day 10. If no nitrification is considered, the mean biodegradation was 75% after 28 days of incubation, the 10 day window was also reached on day 10.
The degradation rate of N-(2,2-Dimethoxyethyl)acrylamide (NAAADA) based on ThODNO3reached 60% within the 10-day window and after 28 days of incubation. According to the guideline OECD 301, N-(2,2-Dimethoxyethyl)-acrylamide (NAAADA) is considered to be readily biodegradable.
The nitrate concentration in the controls after 28 days of incubation was 10.1 mg/L (mean). The nitrate concentration in the test item treated vessels was 44.7 mg/L (mean). Therefore, the process of nitrification occurred. Therefore, the process of nitrification occurred. After correction of the oxygen demand for the nitrification the biodegradation was 48% after 28 days. In consideration of the fact that the biodegradation was 75%(ThODNH4) and 60% (ThODNO3) after 28 days, the biodegradation results should be recognized as readily biodegradable, because the nitrification process is a secondary reaction. The process of nitrification was measured but should not be taken into account to evaluate the biodegradation result.
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