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EC number: 266-257-8 | CAS number: 66215-27-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1994/02 to 1994/08
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EPA OPP 81-3 (Acute inhalation toxicity)
- Version / remarks:
- FIFRA § 10, Subdivision F, 1984
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EU Method B.2 (Acute Toxicity (Inhalation))
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- traditional method
- Limit test:
- no
Test material
- Reference substance name:
- N-cyclopropyl-1,3,5-triazine-2,4,6-triamine
- EC Number:
- 266-257-8
- EC Name:
- N-cyclopropyl-1,3,5-triazine-2,4,6-triamine
- Cas Number:
- 66215-27-8
- Molecular formula:
- C6H10N6
- IUPAC Name:
- N2-cyclopropyl-1,3,5-triazine-2,4,6-triamine
- Test material form:
- solid: particulate/powder
- Remarks:
- Fine white powder
Constituent 1
- Specific details on test material used for the study:
- Storage: Room temperature
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Remarks:
- HSD
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS:
Acclimation Period: At least 4 days
Weight When Tested: Males (179-225 g); Females(195-242 g)
Age: Young adult
Housing: One per cage
ENVIRONMENTAL CONDITIONS:
Temperature Range: 67-77 °F (19.4-25 °C)
Humidity Range: 30-80%
12-hour light/dark cycle
10-12 air changes/hour
Administration / exposure
- Route of administration:
- inhalation: aerosol
- Type of inhalation exposure:
- nose only
- Vehicle:
- air
- Mass median aerodynamic diameter (MMAD):
- >= 3.92 - <= 6.68 µm
- Geometric standard deviation (GSD):
- >= 2.075 - <= 4.142
- Remark on MMAD/GSD:
- The MMAD’s were 6.68 µm ±1.48 for the 3.60 mg/L (3600 mg/m3) concentration and 3.92 µm ±1.48 for the 0.744 mg/L (744 mg/m3) concentration.
GSD ranges: (2.947 to 4.142) at 3.60 mg/L concentration and (2.075 to 4.027) at 0.744 mg/L concentration. - Details on inhalation exposure:
- A 500 L nose-only stainless steel, dynamic flow inhalation chamber was utilized in this experiment. The body of the chamber has 25 ports in 5 rows. Polycarbonate cones are inserted into 10 designated individual ports. The test material is introduced through the opening in the top of the chamber. The bottom section has a corresponding air outlet and a drain valve for cleaning the chamber. The individual polycarbonate cones (tubes) are tapered at one end to t the shape of the animal’s head and the back portion is sealed with a polycarbonate cap. The cones containing the animals fit tightly into the ports, and are sealed with "0" rings.
- Analytical verification of test atmosphere concentrations:
- yes
- Duration of exposure:
- 4 h
- Concentrations:
- The mean concentration was 0.744 or 3.6 mg/L (744 or 3600 mg/m3).
- No. of animals per sex per dose:
- 5
- Control animals:
- yes
- Remarks:
- A control group of 5 rats/sex was exposed to air only under similar experimental conditions.
- Details on study design:
- On Day 0 of the study, 5 Sprague Dawley rats/sex/group were exposed nose-only for a single 4-hour period to an aerosol generated from undiluted cyromazine technical at a mean concentration of 0.744 or 3.6 mg/L (744 or 3600 mg/m3). A control group of 5 rats/sex was exposed to air only under similar experimental conditions. During the 4- hour period 4 samples of the tested atmosphere were taken at regular intervals for the determination of exposure levels. Particle size distribution was also determined during exposure. Clinical signs were recorded twice on the day of exposure and at least daily thereafter for up to 17 days. Body weights were measured prior to exposure, on day 7 and at the day of termination. At termination of the study, all animals were subjected to gross necropsy.
Results and discussion
- Preliminary study:
- Trial assays were conducted using different methods of aerosolizing the test material into the exposure chamber in attempts to determine which method(s) would produce the highest concentration and a mass median aerodynamic diameter (MMAD) between 1 and 4 µm.
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- > 3.6 mg/L air
- Based on:
- test mat.
- Exp. duration:
- 4 h
- Mortality:
- No mortality occurred.
- Clinical signs:
- other: Decreased activity, piloerection at both concentrations, and additionally nasal discharge at the higher concentration tested. All animals appeared normal two days post treatment and for the rest of the study period.
- Body weight:
- Body weight gain was unaffected with the exception of two females exposed to 3.6 mg/L and two control females, which lost weight between days 0 and 7.
- Gross pathology:
- Gross necropsy revealed discoloration of the lungs in animals of all groups.
Any other information on results incl. tables
Table 1:
Exposure parameters
Parameter | Value | |
Low Concentration | High Concentration | |
Nominal concentration | 4.51 mg/L | 45.4 mg/L |
Analytical concentration | 0.7443 mg/L | 3.597 mg/L |
Standard deviation (SD) | 1.48 | 1.48 |
MMAD ± SD (µm) | 3.92 | 6.68 |
% of particles collected | 50% with MMAD < 5.90µm | 50% with MMAD < 3.54µm |
| 84% with MMAD < 23.79µm | 84% with MMAD < 7.35µm |
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The acute inhalation LC50 value for cyromazine in male and female Sprague Dawley rats under the examined conditions was found to be greater than 3.6 mg/L air (maximum attainable concentration).
- Executive summary:
Two groups of Sprague-Dawley rats (5/sex/group) were exposed for four hours to an aerosol generated from undiluted Cyromazine in a nose-only inhalation at a mean concentration of 0.744 or 3.60 mg/L (744 or 3600 mg/m3). A control group consisting of 5 males and 5 females was exposed to room air only for four hours under similar experimental conditions. There was no mortality during the study. Clinical signs of toxicity in the test groups included activity decrease and piloerection at both levels, and nasal discharge at the 3.60 mg/L level. All animals were asymptomatic on Day 2. Body weights were essentially unaffected by exposure, except for two females at the 3.60 mg/L level that lost weight between Days 0 and 7. Control animals appeared normal throughout the study. The gross necropsies of control as well as test animals showed some discoloration of lungs. The acute inhalation LD50 for Cyromazine is greater than the maximum attainable concentration of 3.60 mg/L.
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