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EC number: 806-798-4 | CAS number: 1262985-23-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2011-02-21 to 2011-02-25
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
- Report date:
- 2011
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- A nominal concentration of 100 mg/L was used although its water solubility was 0.2 mg/L. No quantifiable concentrations were found in the test solutions and as such it cannot be confirmed that the test organisms were exposed to the test substance.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- yes
- Remarks:
- A nominal concentration of 100 mg/L was used although its water solubility was 0.2 mg/L. No quantifiable concentrations were found in the test solutions and as such it cannot be confirmed that the test organisms were exposed to the test substance.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- N-{3-[5-(4-chlorophenyl)-1-(2,6-dichlorobenzoyl)-1H-pyrrolo[2,3-b]pyridine-3-carbonyl]-2,4-difluorophenyl}propane-1-sulfonamide
- EC Number:
- 806-798-4
- Cas Number:
- 1262985-23-8
- Molecular formula:
- C30 H20 Cl3 F2 N3 O4 S
- IUPAC Name:
- N-{3-[5-(4-chlorophenyl)-1-(2,6-dichlorobenzoyl)-1H-pyrrolo[2,3-b]pyridine-3-carbonyl]-2,4-difluorophenyl}propane-1-sulfonamide
- Test material form:
- other: solid
- Details on test material:
- Solid
Purity: >98% (w/w)
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- HPLC analyses of the test substance concentrations were conducted in the definitive test at the beginning of the test and after 24 h and 48 h of incubation, respectively.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Prior to the definitive test a non-GLP range finding test with a nominal concentrations of 100 mg/L was performed.
Since no significant effects were observed in this range finding test, only a limit test with the nominal concentration of 100 mg/L was performed as a definitive test. Due to the limited water solubility of the test substance, only the water accommodated fraction was tested.
This water accommodated fraction was prepared by addition of the respective amount of an acetonic stock solution to an empty glass vessel. After complete evaporation of the solvent, the aerated Daphnia medium was added. The test solution was moderately stirred overnight followed by filtration
(MILLIPORE AP15 glass fibre filter). The resulting water accommodated fraction was used in the test.
100 mL of the filtered test solutions were filled in one of the two replicate test vessels. The solution was aerated by stirring for about 0.5 h. 02 concentration and pH was measured. The pH was adjusted if necessary. Afterwards, half of the solution (50 mL) was filled in the 2nd replicate test vessel.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Daphnia maqna (Straus, 1820).
Young daphnia with an age of < 24 hours were selected for the test.
Renewal of the cultures: After 7 days of cultivation half of the medium twice a week.
Temperature o f medium: 20 °C constant within +/-2°C
Feeding: Daily except at weekends.
Illumination: 16 h per day
Medium: Continuously aerated Elendt M4 medium prepared with deionised water (conductivity <1.5 uS/cm).
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- 20°C +/- 2°C
- pH:
- 7.0-7.9
- Dissolved oxygen:
- 5.6-6.1
- Details on test conditions:
- Test type: Static
Test duration: 48 hours
Test vessels: 100 ml, all-glass
Medium: I Continuously aerated Elendt M4 medium; prepared with deionised water (conductivity <1.5 uS/cm
Number of daphnia: 40 per concentration
Loading: 20 per vessel containing 50 ml medium
Light: 16 hours photoperiod daily
Daphnia, aged less than 24 h, were acclimatised to the test medium for about 1 h before introduction into the formulated test media - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.1 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 0.1 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- Using the water accommodated fraction of the test item no toxic effects (:>1 0% immobilization) were observed after 24 and 48 h of exposure. No
toxic effects were also observed in the control after 24 and 48 h of exposure.
Based on the analytically determined concentrations the 24 and 48 h EC50 values were >0.1 mg/L.
The no-observed-effect concentration (NOEC) for 24 and 48 h of incubation based on the analytically determined concentrations were <0.1 mg/L.
100% immobilization was not detected. - Results with reference substance (positive control):
- Periodically acute reference tests were conducted with potassium dichromate
- Reported statistics and error estimates:
- The median effective concentration (EC50 value) is the concentration estimated to immobilize 50% of the Daphnia after 24 or 48 h of exposure. Those individuals not able to swim within 15 s after gentle agitation of the test vessel are considered to be immobile.
The EC values at 24 and 48 h of exposure will be calculated from the percentage response (number of immobile Daphnia) and the logarithms of the corresponding nominal test substance concentrations.
Applicant's summary and conclusion
- Executive summary:
INTRODUCTION: The acute toxicity of the test item to Daphnia magna was investigated under static exposure conditions over a period of 48 h. The applied nominal test concentrations was 100 mg/L. 40 individual Daphnia divided in 2 test vessels were exposed to the test substance.
METHOD: HPLC analyses of the test substance concentrations were conducted at the beginning of the test and after 24 h and 48 h of exposure, respectively.
RESULTS: Using the water accommodated fractions no toxic effects (>10% immobilization) were observed at 24 and 48 h of exposure, respectively. No toxic effects were also observed in the control after 24 and 48 h of exposure. 100% immobilization was not detected. The test substance was not quantified in any of the test solutions, all results were <0.1 mg/L.
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