Registration Dossier
Registration Dossier
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EC number: 906-891-0 | CAS number: -
Toxicity to microorganisms
Administrative data
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study performed according to guideline and under GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: ISO 10712
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- (2-aminoethyl)({2-[(2-aminoethyl)amino]ethyl})amine; (2-aminoethyl)[2-({2-[(2-aminoethyl)amino]ethyl}amino)ethyl]amine; 2-({2-[(2-aminoethyl)amino]ethyl}amino)ethan-1-ol; 2-[(2-hydroxyethyl)amino]ethan-1-ol
- EC Number:
- 906-891-0
- Cas Number:
- 84238-53-9
- Molecular formula:
- UVCB substance and no specific formula
- IUPAC Name:
- (2-aminoethyl)({2-[(2-aminoethyl)amino]ethyl})amine; (2-aminoethyl)[2-({2-[(2-aminoethyl)amino]ethyl}amino)ethyl]amine; 2-({2-[(2-aminoethyl)amino]ethyl}amino)ethan-1-ol; 2-[(2-hydroxyethyl)amino]ethan-1-ol
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Details on test solutions:
- For the purpose of the definitive study the test material was prepared by a direct dispersion in sterile reverse osmosis water.
Test organisms
- Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- The test was carried out using Pseudomonas putida strain NCiB 8248. Freeze dried cultures of Pseudomonas putida were obtained from the National Collection of Industrial Bacteria, Aberdeen, Scotland. Cultures were maintained in the laboratory by routine sub-culturing onto fresh agar slopes approximately once per week. The cultures were maintained in the laboratory at a temperature of 25· C.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 16 h
Test conditions
- Test temperature:
- 25°C
- Nominal and measured concentrations:
- 5.0, 10, 20, 40, 80, 160, 320, 640, 1280, 2560, 5120 and 10240 mg/I , three replicates per soncentration
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 16 h
- Dose descriptor:
- EC10
- Effect conc.:
- 76 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Duration:
- 16 h
- Dose descriptor:
- EC50
- Effect conc.:
- 400 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
